Cholinesterase and Tyrosinase Inhibitory Potential and
Antioxidant Capacity of Lysimachia verticillaris L. and
Isolation of the Major Compounds

J 2020

Cholinesterase and Tyrosinase Inhibitory Potential and Antioxidant Capacity of Lysimachia verticillaris L. and Isolation of the Major Compounds

OZGEN, U.; S. O. SENER; Karel ŠMEJKAL; Jiří VÁCLAVÍK; D. F. SENOL et. al.

Basic information

Original name

Cholinesterase and Tyrosinase Inhibitory Potential and Antioxidant Capacity of Lysimachia verticillaris L. and Isolation of the Major Compounds

Authors

OZGEN, U.; S. O. SENER; Karel ŠMEJKAL (203 Czech Republic, belonging to the institution); Jiří VÁCLAVÍK (203 Czech Republic, belonging to the institution); D. F. SENOL; Orhan I ERDOGAN; Emil ŠVAJDLENKA (203 Czech Republic, belonging to the institution); A. C. GOREN and Milan ŽEMLIČKA (203 Czech Republic, belonging to the institution)

Edition

TURKISH JOURNAL OF PHARMACEUTICAL SCIENCES, CANKAYA-ANKARA, TURKISH PHARMACISTS ASSOC, 2020, 1304-530X

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Turkey

Confidentiality degree

is not subject to a state or trade secret

References:

URL

RIV identification code

RIV/00216224:14160/20:00118239

Organization unit

Faculty of Pharmacy

DOI

https://doi.org/10.4274/tjps.galenos.2019.71598

UT WoS

000585327200011

EID Scopus

2-s2.0-85094626376

Keywords in English

Anticholinesterase; HPLC; isolation; Lysimachia; tyrosinase

Abstract

In the original language

Objectives: The scope of the present study was to specify the therapeutic potential for neurodegenerative diseases through evaluating cholinesterase and tyrosinase (TYR) inhibitory and antioxidant activity of Lysimachia verticillaris (LV), and to isolate the major compounds considering the most active fraction. Materials and Methods: The methanol extract (ME) of LV and the chloroform, ethyl acetate (EtOAC), and aqueous fractions obtained from it were used for biological activity and isolation studies. The ME and all fractions were tested for their acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and TYR inhibitory and antioxidant potentials using ELISA microtiter assays. Seven major compounds were isolated from the active EtOAC fraction by semi-preparative high performance liquid chromatography. The structures of the compounds were elucidated by several spectroscopic methods. Results: Marked AChE inhibitory activity was observed in the EtOAC fraction (6337 +/- 1.74%), BChE inhibitory activity in the ME and EtOAC fraction (85.84 +/- 3.01% and 83.82 +/- 3.93%), total phenol content in the EtOAC fraction (261.59 +/- 3.95 mg equivalent of gallic acid/1 g of extract) and total flavonoid contents in the EtOAC fraction (515.54 +/- 2.80 mg equivalent of quercetin/1 g of extract), and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and ferric-reducing antioxidant power values in the aqueous and EtOAC fractions (92.54 +/- 0.67%, 92.11 +/- 0.30%; 2.318 +/- 0.054, 2.224 +/- 0.091, respectively). Accordingly, the isolation studies were carried out on the EtOAC fractions. Compounds 1-7 (gallic acid, (+)-catechin, myricetin 3-O-arabinofuranoside, myricetin 3-O-alpha-rhamnopyranoside, quercetin 3-O-beta-glucopyranoside, quercetin 3-O-arabinofuranoside, and quercetin 3-O-alpha-rhamnopyranoside, respectively) were isolated from the active EtOAC fraction. Conclusion: LV may be a potential herbal source for treatment of neurodegenerative diseases based on its strong antioxidant activity and significant cholinesterase inhibition similar to that of the reference.

Cite

OZGEN, U.; S. O. SENER; Karel ŠMEJKAL; Jiří VÁCLAVÍK; D. F. SENOL; Orhan I ERDOGAN; Emil ŠVAJDLENKA; A. C. GOREN and Milan ŽEMLIČKA. Cholinesterase and Tyrosinase Inhibitory Potential and Antioxidant Capacity of Lysimachia verticillaris L. and Isolation of the Major Compounds. TURKISH JOURNAL OF PHARMACEUTICAL SCIENCES. CANKAYA-ANKARA: TURKISH PHARMACISTS ASSOC, 2020, vol. 17, No 5, p. 528-534. ISSN 1304-530X. Available from: https://doi.org/10.4274/tjps.galenos.2019.71598.

@article{1745261,
   author = {Ozgen, U. and Sener, S. O. and Šmejkal, Karel and Václavík, Jiří and Senol, D. F. and Erdogan, Orhan I and Švajdlenka, Emil and Goren, A. C. and Žemlička, Milan},
   article_location = {CANKAYA-ANKARA},
   article_number = {5},
   doi = {https://doi.org/10.4274/tjps.galenos.2019.71598},
   keywords = {Anticholinesterase; HPLC; isolation; Lysimachia; tyrosinase},
   language = {eng},
   issn = {1304-530X},
   journal = {TURKISH JOURNAL OF PHARMACEUTICAL SCIENCES},
   title = {Cholinesterase and Tyrosinase Inhibitory Potential and Antioxidant Capacity of Lysimachia verticillaris L. and Isolation of the Major Compounds},
   url = {https://pubmed.ncbi.nlm.nih.gov/33177934/},
   volume = {17},
   year = {2020}
}

TY  - JOUR
ID  - 1745261
AU  - Ozgen, U. - Sener, S. O. - Šmejkal, Karel - Václavík, Jiří - Senol, D. F. - Erdogan, Orhan I - Švajdlenka, Emil - Goren, A. C. - Žemlička, Milan
PY  - 2020
TI  - Cholinesterase and Tyrosinase Inhibitory Potential and Antioxidant Capacity of Lysimachia verticillaris L. and Isolation of the Major Compounds
JF  - TURKISH JOURNAL OF PHARMACEUTICAL SCIENCES
VL  - 17
IS  - 5
SP  - 528-534
EP  - 528-534
PB  - TURKISH PHARMACISTS ASSOC
SN  - 1304530X
KW  - Anticholinesterase
KW  - HPLC
KW  - isolation
KW  - Lysimachia
KW  - tyrosinase
UR  - https://pubmed.ncbi.nlm.nih.gov/33177934/
N2  - Objectives: The scope of the present study was to specify the therapeutic potential for neurodegenerative diseases through evaluating cholinesterase and tyrosinase (TYR) inhibitory and antioxidant activity of Lysimachia verticillaris (LV), and to isolate the major compounds considering the most active fraction. Materials and Methods: The methanol extract (ME) of LV and the chloroform, ethyl acetate (EtOAC), and aqueous fractions obtained from it were used for biological activity and isolation studies. The ME and all fractions were tested for their acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and TYR inhibitory and antioxidant potentials using ELISA microtiter assays. Seven major compounds were isolated from the active EtOAC fraction by semi-preparative high performance liquid chromatography. The structures of the compounds were elucidated by several spectroscopic methods. Results: Marked AChE inhibitory activity was observed in the EtOAC fraction (6337 +/- 1.74%), BChE inhibitory activity in the ME and EtOAC fraction (85.84 +/- 3.01% and 83.82 +/- 3.93%), total phenol content in the EtOAC fraction (261.59 +/- 3.95 mg equivalent of gallic acid/1 g of extract) and total flavonoid contents in the EtOAC fraction (515.54 +/- 2.80 mg equivalent of quercetin/1 g of extract), and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and ferric-reducing antioxidant power values in the aqueous and EtOAC fractions (92.54 +/- 0.67%, 92.11 +/- 0.30%; 2.318 +/- 0.054, 2.224 +/- 0.091, respectively). Accordingly, the isolation studies were carried out on the EtOAC fractions. Compounds 1-7 (gallic acid, (+)-catechin, myricetin 3-O-arabinofuranoside, myricetin 3-O-alpha-rhamnopyranoside, quercetin 3-O-beta-glucopyranoside, quercetin 3-O-arabinofuranoside, and quercetin 3-O-alpha-rhamnopyranoside, respectively) were isolated from the active EtOAC fraction. Conclusion: LV may be a potential herbal source for treatment of neurodegenerative diseases based on its strong antioxidant activity and significant cholinesterase inhibition similar to that of the reference.
ER  -

OZGEN, U.; S. O. SENER; Karel ŠMEJKAL; Jiří VÁCLAVÍK; D. F. SENOL; Orhan I ERDOGAN; Emil ŠVAJDLENKA; A. C. GOREN and Milan ŽEMLIČKA. Cholinesterase and Tyrosinase Inhibitory Potential and Antioxidant Capacity of Lysimachia verticillaris L. and Isolation of the Major Compounds. \textit{TURKISH JOURNAL OF PHARMACEUTICAL SCIENCES}. CANKAYA-ANKARA: TURKISH PHARMACISTS ASSOC, 2020, vol.~17, No~5, p.~528-534. ISSN~1304-530X. Available from: https://doi.org/10.4274/tjps.galenos.2019.71598.

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