YAP-TEAD1 control of cytoskeleton dynamics and intracellular
tension guides human pluripotent stem cell mesoderm
specification

J 2021

YAP-TEAD1 control of cytoskeleton dynamics and intracellular tension guides human pluripotent stem cell mesoderm specification

PAGLIARI, S., Vladimir VINARSKY, Fabiana MARTINO, A. R. PERESTRELO, J. O. DE LA CRUZ et. al.

Basic information

Original name

YAP-TEAD1 control of cytoskeleton dynamics and intracellular tension guides human pluripotent stem cell mesoderm specification

Authors

PAGLIARI, S., Vladimir VINARSKY (203 Czech Republic), Fabiana MARTINO (380 Italy, belonging to the institution), A. R. PERESTRELO, J. O. DE LA CRUZ, Guido CALUORI (380 Italy, belonging to the institution), Jan VRBSKY (203 Czech Republic), P. MOZETIC, A. POMPEIANO, A. ZANCLA, Sri Ranjani GANJI (356 India, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution), Dan KYTYR (203 Czech Republic), Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution), G. GRASSI, M. SAMPAOLESI, A. RAINER and G. FORTE

Edition

Cell Death and Differentiation, London, Springer Nature, 2021, 1350-9047

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 12.067

RIV identification code

RIV/00216224:14740/21:00118867

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1038/s41418-020-00643-5

UT WoS

000584874800002

Keywords in English

NCK-INTERACTING KINASEHIPPO PATHWAYSIGNALING PATHWAYSELF-RENEWALSIZE-CONTROLORGAN SIZEYAPANGIOMOTINHOMEOSTASISDIFFERENTIATION

Abstract

V originále

The tight regulation of cytoskeleton dynamics is required for a number of cellular processes, including migration, division and differentiation. YAP-TEAD respond to cell-cell interaction and to substrate mechanics and, among their downstream effects, prompt focal adhesion (FA) gene transcription, thus contributing to FA-cytoskeleton stability. This activity is key to the definition of adult cell mechanical properties and function. Its regulation and role in pluripotent stem cells are poorly understood. Human PSCs display a sustained basal YAP-driven transcriptional activity despite they grow in very dense colonies, indicating these cells are insensitive to contact inhibition. PSC inability to perceive cell-cell interactions can be restored by tampering with Tankyrase enzyme, thus favouring AMOT inhibition of YAP function. YAP-TEAD complex is promptly inactivated when germ layers are specified, and this event is needed to adjust PSC mechanical properties in response to physiological substrate stiffness. By providing evidence that YAP-TEAD1 complex targets key genes encoding for proteins involved in cytoskeleton dynamics, we suggest that substrate mechanics can direct PSC specification by influencing cytoskeleton arrangement and intracellular tension. We propose an aberrant activation of YAP-TEAD1 axis alters PSC potency by inhibiting cytoskeleton dynamics, thus paralyzing the changes in shape requested for the acquisition of the given phenotype.

Links

GBP206/12/G151, research and development project

Name: Centrum nových přístupů k bioanalýze a molekulární diagnostice

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

90043, large research infrastructures

Name: CIISB

Citovat

PAGLIARI, S., Vladimir VINARSKY, Fabiana MARTINO, A. R. PERESTRELO, J. O. DE LA CRUZ, Guido CALUORI, Jan VRBSKY, P. MOZETIC, A. POMPEIANO, A. ZANCLA, Sri Ranjani GANJI, Petr SKLÁDAL, Dan KYTYR, Zbyněk ZDRÁHAL, G. GRASSI, M. SAMPAOLESI, A. RAINER and G. FORTE. YAP-TEAD1 control of cytoskeleton dynamics and intracellular tension guides human pluripotent stem cell mesoderm specification. Cell Death and Differentiation. London: Springer Nature, 2021, vol. 28, No 4, p. 1193-1207. ISSN 1350-9047. Available from: https://dx.doi.org/10.1038/s41418-020-00643-5.

@article{1745296,
   author = {Pagliari, S. and Vinarsky, Vladimir and Martino, Fabiana and Perestrelo, A. R. and De La Cruz, J. O. and Caluori, Guido and Vrbsky, Jan and Mozetic, P. and Pompeiano, A. and Zancla, A. and Ganji, Sri Ranjani and Skládal, Petr and Kytyr, Dan and Zdráhal, Zbyněk and Grassi, G. and Sampaolesi, M. and Rainer, A. and Forte, G.},
   article_location = {London},
   article_number = {4},
   doi = {http://dx.doi.org/10.1038/s41418-020-00643-5},
   keywords = {NCK-INTERACTING KINASEHIPPO PATHWAYSIGNALING PATHWAYSELF-RENEWALSIZE-CONTROLORGAN SIZEYAPANGIOMOTINHOMEOSTASISDIFFERENTIATION},
   language = {eng},
   issn = {1350-9047},
   journal = {Cell Death and Differentiation},
   title = {YAP-TEAD1 control of cytoskeleton dynamics and intracellular tension guides human pluripotent stem cell mesoderm specification},
   url = {https://www.nature.com/articles/s41418-020-00643-5.pdf},
   volume = {28},
   year = {2021}
}

TY  - JOUR
ID  - 1745296
AU  - Pagliari, S. - Vinarsky, Vladimir - Martino, Fabiana - Perestrelo, A. R. - De La Cruz, J. O. - Caluori, Guido - Vrbsky, Jan - Mozetic, P. - Pompeiano, A. - Zancla, A. - Ganji, Sri Ranjani - Skládal, Petr - Kytyr, Dan - Zdráhal, Zbyněk - Grassi, G. - Sampaolesi, M. - Rainer, A. - Forte, G.
PY  - 2021
TI  - YAP-TEAD1 control of cytoskeleton dynamics and intracellular tension guides human pluripotent stem cell mesoderm specification
JF  - Cell Death and Differentiation
VL  - 28
IS  - 4
SP  - 1193-1207
EP  - 1193-1207
PB  - Springer Nature
SN  - 13509047
KW  - NCK-INTERACTING KINASEHIPPO PATHWAYSIGNALING PATHWAYSELF-RENEWALSIZE-CONTROLORGAN SIZEYAPANGIOMOTINHOMEOSTASISDIFFERENTIATION
UR  - https://www.nature.com/articles/s41418-020-00643-5.pdf
N2  - The tight regulation of cytoskeleton dynamics is required for a number of cellular processes, including migration, division and differentiation. YAP-TEAD respond to cell-cell interaction and to substrate mechanics and, among their downstream effects, prompt focal adhesion (FA) gene transcription, thus contributing to FA-cytoskeleton stability. This activity is key to the definition of adult cell mechanical properties and function. Its regulation and role in pluripotent stem cells are poorly understood. Human PSCs display a sustained basal YAP-driven transcriptional activity despite they grow in very dense colonies, indicating these cells are insensitive to contact inhibition. PSC inability to perceive cell-cell interactions can be restored by tampering with Tankyrase enzyme, thus favouring AMOT inhibition of YAP function. YAP-TEAD complex is promptly inactivated when germ layers are specified, and this event is needed to adjust PSC mechanical properties in response to physiological substrate stiffness. By providing evidence that YAP-TEAD1 complex targets key genes encoding for proteins involved in cytoskeleton dynamics, we suggest that substrate mechanics can direct PSC specification by influencing cytoskeleton arrangement and intracellular tension. We propose an aberrant activation of YAP-TEAD1 axis alters PSC potency by inhibiting cytoskeleton dynamics, thus paralyzing the changes in shape requested for the acquisition of the given phenotype.
ER  -

PAGLIARI, S., Vladimir VINARSKY, Fabiana MARTINO, A. R. PERESTRELO, J. O. DE LA CRUZ, Guido CALUORI, Jan VRBSKY, P. MOZETIC, A. POMPEIANO, A. ZANCLA, Sri Ranjani GANJI, Petr SKLÁDAL, Dan KYTYR, Zbyněk ZDRÁHAL, G. GRASSI, M. SAMPAOLESI, A. RAINER and G. FORTE. YAP-TEAD1 control of cytoskeleton dynamics and intracellular tension guides human pluripotent stem cell mesoderm specification. \textit{Cell Death and Differentiation}. London: Springer Nature, 2021, vol.~28, No~4, p.~1193-1207. ISSN~1350-9047. Available from: https://dx.doi.org/10.1038/s41418-020-00643-5.

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