Upconversion-Linked Immunoassay for the Diagnosis of Honeybee
Disease American Foulbrood

J 2021

Upconversion-Linked Immunoassay for the Diagnosis of Honeybee Disease American Foulbrood

PASTUCHA, Matěj, Eliška ODSTRČILÍKOVÁ, Antonín HLAVÁČEK, Julian BRANDMEIER, Vít VYKOUKAL et. al.

Basic information

Original name

Upconversion-Linked Immunoassay for the Diagnosis of Honeybee Disease American Foulbrood

Authors

PASTUCHA, Matěj (203 Czech Republic, belonging to the institution), Eliška ODSTRČILÍKOVÁ (203 Czech Republic, belonging to the institution), Antonín HLAVÁČEK (203 Czech Republic), Julian BRANDMEIER, Vít VYKOUKAL (203 Czech Republic, belonging to the institution), Julie WEISOVÁ, Hans-Heiner GORRIS (276 Germany), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Zdeněk FARKA (203 Czech Republic, guarantor, belonging to the institution)

Edition

IEEE Journal of Selected Topics in Quantum Electronics, IEEE, 2021, 1077-260X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.653

RIV identification code

RIV/00216224:14310/21:00121176

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1109/JSTQE.2021.3049689

UT WoS

000630043400002

Keywords in English

American foulbrood; bacterial pathogens; Paenibacillus larvae; photon-upconversion nanoparticle; upconversion-linked immunosorbent assay

Abstract

V originále

American foulbrood (AFB) caused by the bacterium Paenibacillus larvae is the most destructive disease of the honeybee brood. Therefore, rapid and sensitive detection methods are required to limit spreading of this pathogen, which has a major impact on agriculture and biodiversity. While P. larvae is typically detected by microbial cultivation or polymerase chain reaction, antibody-based detection represents a viable alternative. Here, we prepared an antibody specific for P. larvae and used it for the development of an upconversion-linked immunosorbent assay (ULISA). Photon-upconversion nanoparticles (UCNP) were conjugated to streptavidin via a PEG-linker using copper-catalyzed click chemistry to replace the enzyme label in conventional enzyme-linked immunosorbent assay (ELISA). The ULISA showed low cross-reactivity and provided a limit of detection of 2.9 × 10 3 CFU/mL, representing a 22-fold improvement compared to the ELISA. This level is within the bacterial loads present in honeybee larvae during an AFB infection. The assay was successfully applied to the analysis of spiked samples of bees, larvae, and hive debris.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

LTAB19011, research and development project

Name: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel

Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION

MUNI/A/1604/2020, interní kód MU

Name: Podpora biochemického výzkumu v roce 2021

Investor: Masaryk University

90110, large research infrastructures

Name: CzechNanoLab

90127, large research infrastructures

Name: CIISB II

Citovat

PASTUCHA, Matěj, Eliška ODSTRČILÍKOVÁ, Antonín HLAVÁČEK, Julian BRANDMEIER, Vít VYKOUKAL, Julie WEISOVÁ, Hans-Heiner GORRIS, Petr SKLÁDAL and Zdeněk FARKA. Upconversion-Linked Immunoassay for the Diagnosis of Honeybee Disease American Foulbrood. IEEE Journal of Selected Topics in Quantum Electronics. IEEE, 2021, vol. 27, No 5, p. 1-11. ISSN 1077-260X. Available from: https://dx.doi.org/10.1109/JSTQE.2021.3049689.

@article{1746445,
   author = {Pastucha, Matěj and Odstrčilíková, Eliška and Hlaváček, Antonín and Brandmeier, Julian and Vykoukal, Vít and Weisová, Julie and Gorris, HansandHeiner and Skládal, Petr and Farka, Zdeněk},
   article_number = {5},
   doi = {http://dx.doi.org/10.1109/JSTQE.2021.3049689},
   keywords = {American foulbrood; bacterial pathogens; Paenibacillus larvae; photon-upconversion nanoparticle; upconversion-linked immunosorbent assay},
   language = {eng},
   issn = {1077-260X},
   journal = {IEEE Journal of Selected Topics in Quantum Electronics},
   title = {Upconversion-Linked Immunoassay for the Diagnosis of Honeybee Disease American Foulbrood},
   url = {https://ieeexplore.ieee.org/document/9314922},
   volume = {27},
   year = {2021}
}

TY  - JOUR
ID  - 1746445
AU  - Pastucha, Matěj - Odstrčilíková, Eliška - Hlaváček, Antonín - Brandmeier, Julian - Vykoukal, Vít - Weisová, Julie - Gorris, Hans-Heiner - Skládal, Petr - Farka, Zdeněk
PY  - 2021
TI  - Upconversion-Linked Immunoassay for the Diagnosis of Honeybee Disease American Foulbrood
JF  - IEEE Journal of Selected Topics in Quantum Electronics
VL  - 27
IS  - 5
SP  - 1-11
EP  - 1-11
PB  - IEEE
SN  - 1077260X
KW  - American foulbrood
KW  - bacterial pathogens
KW  - Paenibacillus larvae
KW  - photon-upconversion nanoparticle
KW  - upconversion-linked immunosorbent assay
UR  - https://ieeexplore.ieee.org/document/9314922
N2  - American foulbrood (AFB) caused by the bacterium Paenibacillus larvae is the most destructive disease of the honeybee brood. Therefore, rapid and sensitive detection methods are required to limit spreading of this pathogen, which has a major impact on agriculture and biodiversity. While P. larvae is typically detected by microbial cultivation or polymerase chain reaction, antibody-based detection represents a viable alternative. Here, we prepared an antibody specific for P. larvae and used it for the development of an upconversion-linked immunosorbent assay (ULISA). Photon-upconversion nanoparticles (UCNP) were conjugated to streptavidin via a PEG-linker using copper-catalyzed click chemistry to replace the enzyme label in conventional enzyme-linked immunosorbent assay (ELISA). The ULISA showed low cross-reactivity and provided a limit of detection of 2.9 × 10 3 CFU/mL, representing a 22-fold improvement compared to the ELISA. This level is within the bacterial loads present in honeybee larvae during an AFB infection. The assay was successfully applied to the analysis of spiked samples of bees, larvae, and hive debris.
ER  -

PASTUCHA, Matěj, Eliška ODSTRČILÍKOVÁ, Antonín HLAVÁČEK, Julian BRANDMEIER, Vít VYKOUKAL, Julie WEISOVÁ, Hans-Heiner GORRIS, Petr SKLÁDAL and Zdeněk FARKA. Upconversion-Linked Immunoassay for the Diagnosis of Honeybee Disease American Foulbrood. \textit{IEEE Journal of Selected Topics in Quantum Electronics}. IEEE, 2021, vol.~27, No~5, p.~1-11. ISSN~1077-260X. Available from: https://dx.doi.org/10.1109/JSTQE.2021.3049689.

Detailed Information on Publication Record