Electroanalysis of unnatural base pair content in plasmid DNA
generated in a semi-synthetic organism

J 2020

Electroanalysis of unnatural base pair content in plasmid DNA generated in a semi-synthetic organism

ŠPAČEK, Jan and Miroslav FOJTA

Basic information

Original name

Electroanalysis of unnatural base pair content in plasmid DNA generated in a semi-synthetic organism

Authors

ŠPAČEK, Jan (203 Czech Republic, belonging to the institution) and Miroslav FOJTA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Electrochimica Acta, OXFORD, Elsevier, 2020, 0013-4686

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.901

RIV identification code

RIV/00216224:14740/20:00114754

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.electacta.2020.137298

UT WoS

000591743600004

Keywords in English

Electrochemistry; Semi-synthetic organism; Unnatural base pair; Catalytic hydrogen evolution; Mercury electrode

Abstract

V originále

While standard Watson-Crick base pairing normally involves hydrogen bonds that join size complemen-tary nitrogenous heterocycles, certain unnatural base pairs (UBP) can support replication, transcription, and translation, including these in semi-synthetic organisms (SSO), using size complementarity alone. In this paper we show that the UBPs can be analyzed electrochemically as components of riboor 2'-deoxyribonucleosides, corresponding (2'-deoxy)triphosphates, as well as incorporated into DNA. Here, performance of the electrochemical methods profits from the ability of the unnatural bases 5SICS and TPT3 adsorbed at the surface of a mercury electrode to catalyze hydrogen evolution in acidic media. This allows semi-quantitative detection of single UBP within plasmid DNA generated in a SSO. This electrochemical approach provides new way for direct, fast, and low cost UBP analysis and can supplement or replace currently used methods of UBP detection. (C) 2020 Elsevier Ltd. All rights reserved.

Links

GBP206/12/G151, research and development project

Name: Centrum nových přístupů k bioanalýze a molekulární diagnostice

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

ŠPAČEK, Jan and Miroslav FOJTA. Electroanalysis of unnatural base pair content in plasmid DNA generated in a semi-synthetic organism. Electrochimica Acta. OXFORD: Elsevier, 2020, vol. 364, DEC, p. 137298-137306. ISSN 0013-4686. Available from: https://dx.doi.org/10.1016/j.electacta.2020.137298.

@article{1748058,
   author = {Špaček, Jan and Fojta, Miroslav},
   article_location = {OXFORD},
   article_number = {DEC},
   doi = {http://dx.doi.org/10.1016/j.electacta.2020.137298},
   keywords = {Electrochemistry; Semi-synthetic organism; Unnatural base pair; Catalytic hydrogen evolution; Mercury electrode},
   language = {eng},
   issn = {0013-4686},
   journal = {Electrochimica Acta},
   title = {Electroanalysis of unnatural base pair content in plasmid DNA generated in a semi-synthetic organism},
   url = {https://www.sciencedirect.com/science/article/pii/S0013468620316911?via%3Dihub},
   volume = {364},
   year = {2020}
}

TY  - JOUR
ID  - 1748058
AU  - Špaček, Jan - Fojta, Miroslav
PY  - 2020
TI  - Electroanalysis of unnatural base pair content in plasmid DNA generated in a semi-synthetic organism
JF  - Electrochimica Acta
VL  - 364
IS  - DEC
SP  - 137298
EP  - 137298
PB  - Elsevier
SN  - 00134686
KW  - Electrochemistry
KW  - Semi-synthetic organism
KW  - Unnatural base pair
KW  - Catalytic hydrogen evolution
KW  - Mercury electrode
UR  - https://www.sciencedirect.com/science/article/pii/S0013468620316911?via%3Dihub
N2  - While standard Watson-Crick base pairing normally involves hydrogen bonds that join size complemen-tary nitrogenous heterocycles, certain unnatural base pairs (UBP) can support replication, transcription, and translation, including these in semi-synthetic organisms (SSO), using size complementarity alone. In this paper we show that the UBPs can be analyzed electrochemically as components of riboor 2'-deoxyribonucleosides, corresponding (2'-deoxy)triphosphates, as well as incorporated into DNA. Here, performance of the electrochemical methods profits from the ability of the unnatural bases 5SICS and TPT3 adsorbed at the surface of a mercury electrode to catalyze hydrogen evolution in acidic media. This allows semi-quantitative detection of single UBP within plasmid DNA generated in a SSO. This electrochemical approach provides new way for direct, fast, and low cost UBP analysis and can supplement or replace currently used methods of UBP detection. (C) 2020 Elsevier Ltd. All rights reserved.
ER  -

ŠPAČEK, Jan and Miroslav FOJTA. Electroanalysis of unnatural base pair content in plasmid DNA generated in a semi-synthetic organism. \textit{Electrochimica Acta}. OXFORD: Elsevier, 2020, vol.~364, DEC, p.~137298-137306. ISSN~0013-4686. Available from: https://dx.doi.org/10.1016/j.electacta.2020.137298.

Detailed Information on Publication Record