Electrodeposited silver amalgam particles on pyrolytic graphite
in (spectro)electrochemical detection of 4-nitrophenol, DNA and
green fluorescent protein

J 2020

Electrodeposited silver amalgam particles on pyrolytic graphite in (spectro)electrochemical detection of 4-nitrophenol, DNA and green fluorescent protein

SEBEST, P.; L. FOJT; V. OSTATNA; Miroslav FOJTA; A. DANHEL et. al.

Základní údaje

Originální název

Electrodeposited silver amalgam particles on pyrolytic graphite in (spectro)electrochemical detection of 4-nitrophenol, DNA and green fluorescent protein

Autoři

SEBEST, P.; L. FOJT; V. OSTATNA; Miroslav FOJTA a A. DANHEL

Vydání

Bioelectrochemistry, SWITZERLAND, Elsevier, 2020, 1567-5394

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.373

Kód RIV

RIV/00216224:14740/20:00118305

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1016/j.bioelechem.2019.107436

UT WoS

000518670600036

EID Scopus

2-s2.0-85076459677

Klíčová slova anglicky

DNA; Electrodeposition; 4-nitrophenol; Protein analysis; Silver amalgam particles; Spectroelectrochemistry

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 3. 2021 17:43, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Catalytic properties and high adsorption affinity of nucleic acids and proteins to silver amalgam electrode surface make this kind of electrified interface perspective for bioanalytical and biomedical applications. For the first time, a basal-plane pyrolytic graphite electrode (bPGE) has been used as a substrate for electrodeposition of silver amalgam particles (AgAPs). Optimization of the resulting composition, surface morphology and electrochemical properties of the AgAPs was done by scanning electron microscopy with energy disperse X-ray spectroscopy, image processing software and voltammetric detection of electrochemically reducible model organic nitro compound, 4-nitrophenol. Spectro-electrochemical applicability of bPGE-AgAP has been demonstrated by electrolysis of 4-nitrophenol. Simultaneous UV-Vis-chronoamperometry provided information on the number of exchange electrons and the reduction rate constants. Preferential adsorption of the fluorescently labelled calf thymus DNA and the green fluorescent protein (GFP) on the surface of AgAPs was observed by fluorescence microscopy. In contrast to previously studied indium-tin oxide and vapour-deposited gold decorated by AgAPs, herein the presented bPGE-AgAP has provided sufficiently wide negative potential window allowing direct electroanalysis of non labelled DNA and GFP using intrinsic electrochemical signals independently of the fluorescent labelling. The bPGE-AgAP can thus be expected to find application opportunities in protein electrochemistry, (bio) sensor development or in-situ spectro-electrochemical studies. (C) 2019 Elsevier B.V. All rights reserved.

Citovat

SEBEST, P.; L. FOJT; V. OSTATNA; Miroslav FOJTA a A. DANHEL. Electrodeposited silver amalgam particles on pyrolytic graphite in (spectro)electrochemical detection of 4-nitrophenol, DNA and green fluorescent protein. Bioelectrochemistry. SWITZERLAND: Elsevier, 2020, roč. 132, APR, s. 132-140. ISSN 1567-5394. Dostupné z: https://doi.org/10.1016/j.bioelechem.2019.107436.

@article{1748060,
   author = {Sebest, P. and Fojt, L. and Ostatna, V. and Fojta, Miroslav and Danhel, A.},
   article_location = {SWITZERLAND},
   article_number = {APR},
   doi = {https://doi.org/10.1016/j.bioelechem.2019.107436},
   keywords = {DNA; Electrodeposition; 4-nitrophenol; Protein analysis; Silver amalgam particles; Spectroelectrochemistry},
   language = {eng},
   issn = {1567-5394},
   journal = {Bioelectrochemistry},
   title = {Electrodeposited silver amalgam particles on pyrolytic graphite in (spectro)electrochemical detection of 4-nitrophenol, DNA and green fluorescent protein},
   url = {https://www.sciencedirect.com/science/article/pii/S1567539419304098?via%3Dihub},
   volume = {132},
   year = {2020}
}

TY  - JOUR
ID  - 1748060
AU  - Sebest, P. - Fojt, L. - Ostatna, V. - Fojta, Miroslav - Danhel, A.
PY  - 2020
TI  - Electrodeposited silver amalgam particles on pyrolytic graphite in (spectro)electrochemical detection of 4-nitrophenol, DNA and green fluorescent protein
JF  - Bioelectrochemistry
VL  - 132
IS  - APR
SP  - 132
EP  - 132
PB  - Elsevier
SN  - 15675394
KW  - DNA
KW  - Electrodeposition
KW  - 4-nitrophenol
KW  - Protein analysis
KW  - Silver amalgam particles
KW  - Spectroelectrochemistry
UR  - https://www.sciencedirect.com/science/article/pii/S1567539419304098?via%3Dihub
N2  - Catalytic properties and high adsorption affinity of nucleic acids and proteins to silver amalgam electrode surface make this kind of electrified interface perspective for bioanalytical and biomedical applications. For the first time, a basal-plane pyrolytic graphite electrode (bPGE) has been used as a substrate for electrodeposition of silver amalgam particles (AgAPs). Optimization of the resulting composition, surface morphology and electrochemical properties of the AgAPs was done by scanning electron microscopy with energy disperse X-ray spectroscopy, image processing software and voltammetric detection of electrochemically reducible model organic nitro compound, 4-nitrophenol. Spectro-electrochemical applicability of bPGE-AgAP has been demonstrated by electrolysis of 4-nitrophenol. Simultaneous UV-Vis-chronoamperometry provided information on the number of exchange electrons and the reduction rate constants. Preferential adsorption of the fluorescently labelled calf thymus DNA and the green fluorescent protein (GFP) on the surface of AgAPs was observed by fluorescence microscopy. In contrast to previously studied indium-tin oxide and vapour-deposited gold decorated by AgAPs, herein the presented bPGE-AgAP has provided sufficiently wide negative potential window allowing direct electroanalysis of non labelled DNA and GFP using intrinsic electrochemical signals independently of the fluorescent labelling. The bPGE-AgAP can thus be expected to find application opportunities in protein electrochemistry, (bio) sensor development or in-situ spectro-electrochemical studies. (C) 2019 Elsevier B.V. All rights reserved.
ER  -

SEBEST, P.; L. FOJT; V. OSTATNA; Miroslav FOJTA a A. DANHEL. Electrodeposited silver amalgam particles on pyrolytic graphite in (spectro)electrochemical detection of 4-nitrophenol, DNA and green fluorescent protein. \textit{Bioelectrochemistry}. SWITZERLAND: Elsevier, 2020, roč.~132, APR, s.~132-140. ISSN~1567-5394. Dostupné z: https://doi.org/10.1016/j.bioelechem.2019.107436.

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