Elimination of Viroids from Tobacco Pollen Involves a Decrease
in Propagation Rate and an Increase of the Degradation
Processes

J 2020

Elimination of Viroids from Tobacco Pollen Involves a Decrease in Propagation Rate and an Increase of the Degradation Processes

MATOUSEK, J., L. STEINBACHOVA, L.Z. DRABKOVA, T. KOCABEK, David POTĚŠIL et. al.

Basic information

Original name

Elimination of Viroids from Tobacco Pollen Involves a Decrease in Propagation Rate and an Increase of the Degradation Processes

Authors

MATOUSEK, J., L. STEINBACHOVA, L.Z. DRABKOVA, T. KOCABEK, David POTĚŠIL (203 Czech Republic, guarantor, belonging to the institution), A.K. MISHRA, D. HONYS and G. STEGER

Edition

International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2020, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.923

RIV identification code

RIV/00216224:14740/20:00118307

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3390/ijms21083029

UT WoS

000535565300371

Keywords in English

AFCVd and CBCVd propagation and eradication; viroid replication; viroid degradation; Nicotiana tabacum; male gametophyte; small RNA; recombinant AGO; TUDOR S-nuclease; strand-specific viroid RT-qPCR

Abstract

V originále

Some viroids-single-stranded, non-coding, circular RNA parasites of plants-are not transmissible through pollen to seeds and to next generation. We analyzed the cause for the elimination of apple fruit crinkle viroid (AFCVd) and citrus bark cracking viroid (CBCVd) from male gametophyte cells of Nicotiana tabacum by RNA deep sequencing and molecular methods using infected and transformed tobacco pollen tissues at different developmental stages. AFCVd was not transferable from pollen to seeds in reciprocal pollinations, due to a complete viroid eradication during the last steps of pollen development and fertilization. In pollen, the viroid replication pathway proceeds with detectable replication intermediates, but is dramatically depressed in comparison to leaves. Specific and unspecific viroid degradation with some preference for (-) chains occurred in pollen, as detected by analysis of viroid-derived small RNAs, by quantification of viroid levels and by detection of viroid degradation products forming "comets" on Northern blots. The decrease of viroid levels during pollen development correlated with mRNA accumulation of several RNA-degrading factors, such as AGO5 nuclease, DICER-like and TUDOR S-like nuclease. In addition, the functional status of pollen, as a tissue with high ribosome content, could play a role during suppression of AFCVd replication involving transcription factors IIIA and ribosomal protein L5.

Links

LM2018140, research and development project

Name: e-Infrastruktura CZ (Acronym: e-INFRA CZ)

Investor: Ministry of Education, Youth and Sports of the CR

90127, large research infrastructures

Name: CIISB II

Citovat

MATOUSEK, J., L. STEINBACHOVA, L.Z. DRABKOVA, T. KOCABEK, David POTĚŠIL, A.K. MISHRA, D. HONYS and G. STEGER. Elimination of Viroids from Tobacco Pollen Involves a Decrease in Propagation Rate and an Increase of the Degradation Processes. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2020, vol. 21, No 8, p. 3029-3054. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms21083029.

@article{1748079,
   author = {Matousek, J. and Steinbachova, L. and Drabkova, L.Z. and Kocabek, T. and Potěšil, David and Mishra, A.K. and Honys, D. and Steger, G.},
   article_location = {Basel},
   article_number = {8},
   doi = {http://dx.doi.org/10.3390/ijms21083029},
   keywords = {AFCVd and CBCVd propagation and eradication; viroid replication; viroid degradation; Nicotiana tabacum; male gametophyte; small RNA; recombinant AGO; TUDOR S-nuclease; strand-specific viroid RT-qPCR},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Elimination of Viroids from Tobacco Pollen Involves a Decrease in Propagation Rate and an Increase of the Degradation Processes},
   url = {https://www.mdpi.com/1422-0067/21/8/3029},
   volume = {21},
   year = {2020}
}

TY  - JOUR
ID  - 1748079
AU  - Matousek, J. - Steinbachova, L. - Drabkova, L.Z. - Kocabek, T. - Potěšil, David - Mishra, A.K. - Honys, D. - Steger, G.
PY  - 2020
TI  - Elimination of Viroids from Tobacco Pollen Involves a Decrease in Propagation Rate and an Increase of the Degradation Processes
JF  - International Journal of Molecular Sciences
VL  - 21
IS  - 8
SP  - 3029
EP  - 3029
PB  - Multidisciplinary Digital Publishing Institute
SN  - 14220067
KW  - AFCVd and CBCVd propagation and eradication
KW  - viroid replication
KW  - viroid degradation
KW  - Nicotiana tabacum
KW  - male gametophyte
KW  - small RNA
KW  - recombinant AGO
KW  - TUDOR S-nuclease
KW  - strand-specific viroid RT-qPCR
UR  - https://www.mdpi.com/1422-0067/21/8/3029
N2  - Some viroids-single-stranded, non-coding, circular RNA parasites of plants-are not transmissible through pollen to seeds and to next generation. We analyzed the cause for the elimination of apple fruit crinkle viroid (AFCVd) and citrus bark cracking viroid (CBCVd) from male gametophyte cells of Nicotiana tabacum by RNA deep sequencing and molecular methods using infected and transformed tobacco pollen tissues at different developmental stages. AFCVd was not transferable from pollen to seeds in reciprocal pollinations, due to a complete viroid eradication during the last steps of pollen development and fertilization. In pollen, the viroid replication pathway proceeds with detectable replication intermediates, but is dramatically depressed in comparison to leaves. Specific and unspecific viroid degradation with some preference for (-) chains occurred in pollen, as detected by analysis of viroid-derived small RNAs, by quantification of viroid levels and by detection of viroid degradation products forming "comets" on Northern blots. The decrease of viroid levels during pollen development correlated with mRNA accumulation of several RNA-degrading factors, such as AGO5 nuclease, DICER-like and TUDOR S-like nuclease. In addition, the functional status of pollen, as a tissue with high ribosome content, could play a role during suppression of AFCVd replication involving transcription factors IIIA and ribosomal protein L5.
ER  -

MATOUSEK, J., L. STEINBACHOVA, L.Z. DRABKOVA, T. KOCABEK, David POTĚŠIL, A.K. MISHRA, D. HONYS and G. STEGER. Elimination of Viroids from Tobacco Pollen Involves a Decrease in Propagation Rate and an Increase of the Degradation Processes. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2020, vol.~21, No~8, p.~3029-3054. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms21083029.

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