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@article{1748817,
author = {Rosati, E. and Pogorelyy, M.V. and Dowds, C.M. and Moller, F.T. and Sorensen, S.B. and Lebedev, Y.B. and Frey, N. and Schreiber, S. and Spehlmann, M.E. and Andersen, V. and Mamedov, Ilgar and Franke, A.},
article_location = {OXFORD},
article_number = {6},
doi = {http://dx.doi.org/10.1093/ecco-jcc/jjz179},
keywords = {T cell receptor [TCR] repertoire; monozygotic twins; inflammatory bowel diseases [IBD]},
language = {eng},
issn = {1873-9946},
journal = {JOURNAL OF CROHNS & COLITIS},
title = {Identification of Disease-associated Traits and Clonotypes in the T Cell Receptor Repertoire of Monozygotic Twins Affected by Inflammatory Bowel Diseases},
url = {https://academic.oup.com/ecco-jcc/article/14/6/778/5621358},
volume = {14},
year = {2020}
}
TY - JOUR
ID - 1748817
AU - Rosati, E. - Pogorelyy, M.V. - Dowds, C.M. - Moller, F.T. - Sorensen, S.B. - Lebedev, Y.B. - Frey, N. - Schreiber, S. - Spehlmann, M.E. - Andersen, V. - Mamedov, Ilgar - Franke, A.
PY - 2020
TI - Identification of Disease-associated Traits and Clonotypes in the T Cell Receptor Repertoire of Monozygotic Twins Affected by Inflammatory Bowel Diseases
JF - JOURNAL OF CROHNS & COLITIS
VL - 14
IS - 6
SP - 778-790
EP - 778-790
PB - OXFORD UNIV PRESS
SN - 18739946
KW - T cell receptor [TCR] repertoire
KW - monozygotic twins
KW - inflammatory bowel diseases [IBD]
UR - https://academic.oup.com/ecco-jcc/article/14/6/778/5621358
N2 - Background and Aims: Intestinal inflammation in inflammatory bowel diseases [IBD] is thought to beT cell mediated and therefore dependent on the interaction between the T cell receptor [TCR] and human leukocyte antigen [HLA] proteins expressed on antigen presenting cells. The collection of all TCRs in one individual, known as the TCR repertoire, is characterised by enormous diversity and inter-individual variability. It was shown that healthy monozygotic [MZ] twins are more similar in theirTCR repertoire than unrelated individuals. Therefore MZ twins, concordant or discordant for IBD, may be useful to identify disease-related and non-genetic factors in the TCR repertoire which could potentially be used as disease biomarkers. Methods: Employing unique molecular barcoding that can distinguish between polymerase chain reaction [PCR] artefacts and true sequence variation, we performed deep TCR alpha and TCR beta repertoire profiling of the peripheral blood of 28 MZ twin pairs from Denmark and Germany, 24 of whom were discordant and four concordant for IBD. Results: We observed disease- and smoking-associated traits such as sharing, diversity and abundance of specific clonotypes in the TCR repertoire of IBD patients, and particularly in patients with active disease, compared with their healthy twins. Conclusions: Our findings identified TCR repertoire features specific for smokers and IBD patients, particularly when signs of disease activity were present. These findings are a first step towards the application of TCR repertoire analyses as a valuable tool to characterise inflammatory bowel diseases and to identify potential biomarkers and true disease causes.
ER -
ROSATI, E., M.V. POGORELYY, C.M. DOWDS, F.T. MOLLER, S.B. SORENSEN, Y.B. LEBEDEV, N. FREY, S. SCHREIBER, M.E. SPEHLMANN, V. ANDERSEN, Ilgar MAMEDOV and A. FRANKE. Identification of Disease-associated Traits and Clonotypes in the T Cell Receptor Repertoire of Monozygotic Twins Affected by Inflammatory Bowel Diseases. \textit{JOURNAL OF CROHNS \&{}amp; COLITIS}. OXFORD: OXFORD UNIV PRESS, 2020, vol.~14, No~6, p.~778-790. ISSN~1873-9946. Available from: https://dx.doi.org/10.1093/ecco-jcc/jjz179.
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