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@article{1748856, author = {Valuchová, Soňa and Mikulková, Pavlína and Pečinková, Jana and Klimova, J. and Krumnikl, M. and Bainar, P. and Heckmann, S. and Tomancak, P. and Říha, Karel}, article_location = {CAMBRIDGE}, article_number = {FEB}, doi = {http://dx.doi.org/10.7554/eLife.52546}, keywords = {OPTICAL MANIPULATION; MOLECULAR-BIOLOGY; MEIOTIC PROPHASE; THALIANA; MEIOSIS; VISUALIZATION; SEGREGATION; EVOLUTION; PROGRAM; TAPETUM}, language = {eng}, issn = {2050-084X}, journal = {elife}, title = {Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy}, url = {https://elifesciences.org/articles/52546}, volume = {9}, year = {2020} }
TY - JOUR ID - 1748856 AU - Valuchová, Soňa - Mikulková, Pavlína - Pečinková, Jana - Klimova, J. - Krumnikl, M. - Bainar, P. - Heckmann, S. - Tomancak, P. - Říha, Karel PY - 2020 TI - Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy JF - elife VL - 9 IS - FEB SP - 52546 EP - 52546 PB - ELIFE SCIENCES PUBLICATIONS LTD SN - 2050084X KW - OPTICAL MANIPULATION KW - MOLECULAR-BIOLOGY KW - MEIOTIC PROPHASE KW - THALIANA KW - MEIOSIS KW - VISUALIZATION KW - SEGREGATION KW - EVOLUTION KW - PROGRAM KW - TAPETUM UR - https://elifesciences.org/articles/52546 N2 - In higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes. This is largely because the germline is restricted to relatively few cells buried deep within floral tissues, which makes them difficult to study. To overcome this limitation, we have developed a methodology for live imaging of the germ cell lineage within floral organs of Arabidopsis using light sheet fluorescence microscopy. We have established reporter lines, cultivation conditions, and imaging protocols for high-resolution microscopy of developing flowers continuously for up to several days. We used multiview imagining to reconstruct a three-dimensional model of a flower at subcellular resolution. We demonstrate the power of this approach by capturing male and female meiosis, asymmetric pollen division, movement of meiotic chromosomes, and unusual restitution mitosis in tapetum cells. This method will enable new avenues of research into plant sexual reproduction. ER -
VALUCHOVÁ, Soňa, Pavlína MIKULKOVÁ, Jana PEČINKOVÁ, J. KLIMOVA, M. KRUMNIKL, P. BAINAR, S. HECKMANN, P. TOMANCAK and Karel ŘÍHA. Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy. \textit{elife}. CAMBRIDGE: ELIFE SCIENCES PUBLICATIONS LTD, 2020, vol.~9, FEB, p.~52546-52564. ISSN~2050-084X. Available from: https://dx.doi.org/10.7554/eLife.52546.
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