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@article{1748856,
author = {Valuchová, Soňa and Mikulková, Pavlína and Pečinková, Jana and Klimova, J. and Krumnikl, M. and Bainar, P. and Heckmann, S. and Tomancak, P. and Říha, Karel},
article_location = {CAMBRIDGE},
article_number = {FEB},
doi = {http://dx.doi.org/10.7554/eLife.52546},
keywords = {OPTICAL MANIPULATION; MOLECULAR-BIOLOGY; MEIOTIC PROPHASE; THALIANA; MEIOSIS; VISUALIZATION; SEGREGATION; EVOLUTION; PROGRAM; TAPETUM},
language = {eng},
issn = {2050-084X},
journal = {elife},
title = {Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy},
url = {https://elifesciences.org/articles/52546},
volume = {9},
year = {2020}
}
TY - JOUR
ID - 1748856
AU - Valuchová, Soňa - Mikulková, Pavlína - Pečinková, Jana - Klimova, J. - Krumnikl, M. - Bainar, P. - Heckmann, S. - Tomancak, P. - Říha, Karel
PY - 2020
TI - Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy
JF - elife
VL - 9
IS - FEB
SP - 52546
EP - 52546
PB - ELIFE SCIENCES PUBLICATIONS LTD
SN - 2050084X
KW - OPTICAL MANIPULATION
KW - MOLECULAR-BIOLOGY
KW - MEIOTIC PROPHASE
KW - THALIANA
KW - MEIOSIS
KW - VISUALIZATION
KW - SEGREGATION
KW - EVOLUTION
KW - PROGRAM
KW - TAPETUM
UR - https://elifesciences.org/articles/52546
N2 - In higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes. This is largely because the germline is restricted to relatively few cells buried deep within floral tissues, which makes them difficult to study. To overcome this limitation, we have developed a methodology for live imaging of the germ cell lineage within floral organs of Arabidopsis using light sheet fluorescence microscopy. We have established reporter lines, cultivation conditions, and imaging protocols for high-resolution microscopy of developing flowers continuously for up to several days. We used multiview imagining to reconstruct a three-dimensional model of a flower at subcellular resolution. We demonstrate the power of this approach by capturing male and female meiosis, asymmetric pollen division, movement of meiotic chromosomes, and unusual restitution mitosis in tapetum cells. This method will enable new avenues of research into plant sexual reproduction.
ER -
VALUCHOVÁ, Soňa, Pavlína MIKULKOVÁ, Jana PEČINKOVÁ, J. KLIMOVA, M. KRUMNIKL, P. BAINAR, S. HECKMANN, P. TOMANCAK and Karel ŘÍHA. Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy. \textit{elife}. CAMBRIDGE: ELIFE SCIENCES PUBLICATIONS LTD, 2020, vol.~9, FEB, p.~52546-52564. ISSN~2050-084X. Available from: https://dx.doi.org/10.7554/eLife.52546.
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