Detailed Information on Publication Record
2020
Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy
VALUCHOVÁ, Soňa, Pavlína MIKULKOVÁ, Jana PEČINKOVÁ, J. KLIMOVA, M. KRUMNIKL et. al.Basic information
Original name
Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy
Authors
VALUCHOVÁ, Soňa (703 Slovakia, belonging to the institution), Pavlína MIKULKOVÁ (203 Czech Republic, belonging to the institution), Jana PEČINKOVÁ (203 Czech Republic, belonging to the institution), J. KLIMOVA, M. KRUMNIKL, P. BAINAR, S. HECKMANN, P. TOMANCAK and Karel ŘÍHA (203 Czech Republic, guarantor, belonging to the institution)
Edition
elife, CAMBRIDGE, ELIFE SCIENCES PUBLICATIONS LTD, 2020, 2050-084X
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10611 Plant sciences, botany
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 8.140
RIV identification code
RIV/00216224:14740/20:00118319
Organization unit
Central European Institute of Technology
UT WoS
000514104400001
Keywords in English
OPTICAL MANIPULATION; MOLECULAR-BIOLOGY; MEIOTIC PROPHASE; THALIANA; MEIOSIS; VISUALIZATION; SEGREGATION; EVOLUTION; PROGRAM; TAPETUM
Tags
International impact, Reviewed
Změněno: 9/10/2024 12:50, Mgr. Adéla Pešková
Abstract
V originále
In higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes. This is largely because the germline is restricted to relatively few cells buried deep within floral tissues, which makes them difficult to study. To overcome this limitation, we have developed a methodology for live imaging of the germ cell lineage within floral organs of Arabidopsis using light sheet fluorescence microscopy. We have established reporter lines, cultivation conditions, and imaging protocols for high-resolution microscopy of developing flowers continuously for up to several days. We used multiview imagining to reconstruct a three-dimensional model of a flower at subcellular resolution. We demonstrate the power of this approach by capturing male and female meiosis, asymmetric pollen division, movement of meiotic chromosomes, and unusual restitution mitosis in tapetum cells. This method will enable new avenues of research into plant sexual reproduction.
Links
EF15_003/0000479, research and development project |
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90062, large research infrastructures |
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