J 2020

Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy

VALUCHOVÁ, Soňa, Pavlína MIKULKOVÁ, Jana PEČINKOVÁ, J. KLIMOVA, M. KRUMNIKL et. al.

Basic information

Original name

Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy

Authors

VALUCHOVÁ, Soňa (703 Slovakia, belonging to the institution), Pavlína MIKULKOVÁ (203 Czech Republic, belonging to the institution), Jana PEČINKOVÁ (203 Czech Republic, belonging to the institution), J. KLIMOVA, M. KRUMNIKL, P. BAINAR, S. HECKMANN, P. TOMANCAK and Karel ŘÍHA (203 Czech Republic, guarantor, belonging to the institution)

Edition

elife, CAMBRIDGE, ELIFE SCIENCES PUBLICATIONS LTD, 2020, 2050-084X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 8.140

RIV identification code

RIV/00216224:14740/20:00118319

Organization unit

Central European Institute of Technology

UT WoS

000514104400001

Keywords in English

OPTICAL MANIPULATION; MOLECULAR-BIOLOGY; MEIOTIC PROPHASE; THALIANA; MEIOSIS; VISUALIZATION; SEGREGATION; EVOLUTION; PROGRAM; TAPETUM

Tags

International impact, Reviewed
Změněno: 9/10/2024 12:50, Mgr. Adéla Pešková

Abstract

V originále

In higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes. This is largely because the germline is restricted to relatively few cells buried deep within floral tissues, which makes them difficult to study. To overcome this limitation, we have developed a methodology for live imaging of the germ cell lineage within floral organs of Arabidopsis using light sheet fluorescence microscopy. We have established reporter lines, cultivation conditions, and imaging protocols for high-resolution microscopy of developing flowers continuously for up to several days. We used multiview imagining to reconstruct a three-dimensional model of a flower at subcellular resolution. We demonstrate the power of this approach by capturing male and female meiosis, asymmetric pollen division, movement of meiotic chromosomes, and unusual restitution mitosis in tapetum cells. This method will enable new avenues of research into plant sexual reproduction.

Links

EF15_003/0000479, research and development project
Name: Regulace rostlinné meiózy
90062, large research infrastructures
Name: Czech-BioImaging