Karyotype changes in long-term cultured tick cell lines

J 2020

Karyotype changes in long-term cultured tick cell lines

KOTSARENKO, Kateryna, P. VECHTOVA, J. LIESKOVSKA, Z. FUSSY, D.C. CABRAL-DE-MELLO et. al.

Basic information

Original name

Karyotype changes in long-term cultured tick cell lines

Authors

KOTSARENKO, Kateryna (804 Ukraine, guarantor, belonging to the institution), P. VECHTOVA, J. LIESKOVSKA, Z. FUSSY, D.C. CABRAL-DE-MELLO, R.O.M. REGO, P. ALBERDI, M. COLLINS, L. BELL-SAKYI, J. STERBA and L. GRUBHOFFER

Edition

Nature Scientific Reports, London, NATURE RESEARCH, 2020, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.379

RIV identification code

RIV/00216224:14740/20:00118329

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1038/s41598-020-70330-5

UT WoS

000561123700019

Keywords in English

TRANSGENE EXPRESSION; IXODES-SCAPULARIS; GENOME SIZE; ACARI; EVOLUTION; INSECT; ESTABLISHMENT; ADAPTATION; DISCOVERY; REPEATS

Abstract

V originále

Tick cell lines are an easy-to-handle system for the study of viral and bacterial infections and other aspects of tick cellular processes. Tick cell cultures are often continuously cultivated, as freezing can affect their viability. However, the long-term cultivation of tick cells can influence their genome stability. In the present study, we investigated karyotype and genome size of tick cell lines. Though 16S rDNA sequencing showed the similarity between Ixodes spp. cell lines at different passages, their karyotypes differed from 2n=28 chromosomes for parental Ixodes spp. ticks, and both increase and decrease in chromosome numbers were observed. For example, the highly passaged Ixodes scapularis cell line ISE18 and Ixodes ricinus cell lines IRE/CTVM19 and IRE/CTVM20 had modal chromosome numbers 48, 23 and 48, respectively. Also, the Ornithodoros moubata cell line OME/CTVM22 had the modal chromosome number 33 instead of 2n=20 chromosomes for Ornithodoros spp. ticks. All studied tick cell lines had a larger genome size in comparison to the genomes of the parental ticks. Thus, highly passaged tick cell lines can be used for research purposes, but possible differences in encoded genetic information and downstream cellular processes, between different cell populations, should be taken into account.

Links

LM2015085, research and development project

Name: CERIT Scientific Cloud (Acronym: CERIT-SC)

Investor: Ministry of Education, Youth and Sports of the CR, CERIT Scientific Cloud

Citovat

KOTSARENKO, Kateryna, P. VECHTOVA, J. LIESKOVSKA, Z. FUSSY, D.C. CABRAL-DE-MELLO, R.O.M. REGO, P. ALBERDI, M. COLLINS, L. BELL-SAKYI, J. STERBA and L. GRUBHOFFER. Karyotype changes in long-term cultured tick cell lines. Nature Scientific Reports. London: NATURE RESEARCH, 2020, vol. 10, No 1, p. 13443-13454. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-020-70330-5.

@article{1749397,
   author = {Kotsarenko, Kateryna and Vechtova, P. and Lieskovska, J. and Fussy, Z. and CabralanddeandMello, D.C. and Rego, R.O.M. and Alberdi, P. and Collins, M. and BellandSakyi, L. and Sterba, J. and Grubhoffer, L.},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-020-70330-5},
   keywords = {TRANSGENE EXPRESSION; IXODES-SCAPULARIS; GENOME SIZE; ACARI; EVOLUTION; INSECT; ESTABLISHMENT; ADAPTATION; DISCOVERY; REPEATS},
   language = {eng},
   issn = {2045-2322},
   journal = {Nature Scientific Reports},
   title = {Karyotype changes in long-term cultured tick cell lines},
   url = {https://www.nature.com/articles/s41598-020-70330-5},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1749397
AU  - Kotsarenko, Kateryna - Vechtova, P. - Lieskovska, J. - Fussy, Z. - Cabral-de-Mello, D.C. - Rego, R.O.M. - Alberdi, P. - Collins, M. - Bell-Sakyi, L. - Sterba, J. - Grubhoffer, L.
PY  - 2020
TI  - Karyotype changes in long-term cultured tick cell lines
JF  - Nature Scientific Reports
VL  - 10
IS  - 1
SP  - 13443
EP  - 13443
PB  - NATURE RESEARCH
SN  - 20452322
KW  - TRANSGENE EXPRESSION
KW  - IXODES-SCAPULARIS
KW  - GENOME SIZE
KW  - ACARI
KW  - EVOLUTION
KW  - INSECT
KW  - ESTABLISHMENT
KW  - ADAPTATION
KW  - DISCOVERY
KW  - REPEATS
UR  - https://www.nature.com/articles/s41598-020-70330-5
N2  - Tick cell lines are an easy-to-handle system for the study of viral and bacterial infections and other aspects of tick cellular processes. Tick cell cultures are often continuously cultivated, as freezing can affect their viability. However, the long-term cultivation of tick cells can influence their genome stability. In the present study, we investigated karyotype and genome size of tick cell lines. Though 16S rDNA sequencing showed the similarity between Ixodes spp. cell lines at different passages, their karyotypes differed from 2n=28 chromosomes for parental Ixodes spp. ticks, and both increase and decrease in chromosome numbers were observed. For example, the highly passaged Ixodes scapularis cell line ISE18 and Ixodes ricinus cell lines IRE/CTVM19 and IRE/CTVM20 had modal chromosome numbers 48, 23 and 48, respectively. Also, the Ornithodoros moubata cell line OME/CTVM22 had the modal chromosome number 33 instead of 2n=20 chromosomes for Ornithodoros spp. ticks. All studied tick cell lines had a larger genome size in comparison to the genomes of the parental ticks. Thus, highly passaged tick cell lines can be used for research purposes, but possible differences in encoded genetic information and downstream cellular processes, between different cell populations, should be taken into account.
ER  -

KOTSARENKO, Kateryna, P. VECHTOVA, J. LIESKOVSKA, Z. FUSSY, D.C. CABRAL-DE-MELLO, R.O.M. REGO, P. ALBERDI, M. COLLINS, L. BELL-SAKYI, J. STERBA and L. GRUBHOFFER. Karyotype changes in long-term cultured tick cell lines. \textit{Nature Scientific Reports}. London: NATURE RESEARCH, 2020, vol.~10, No~1, p.~13443-13454. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-020-70330-5.

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