Molecularly imprinted polymers and capillary electrophoresis
for sensing phytoestrogens in milk

J 2020

Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk

BEZDEKOVA, J., M. VLCNOVSKA, K. ZEMANKOVA, R. BACOVA, M. KOLACKOVA et. al.

Basic information

Original name

Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk

Authors

BEZDEKOVA, J., M. VLCNOVSKA, K. ZEMANKOVA, R. BACOVA, M. KOLACKOVA, T. LEDNICKY, Jan PŘIBYL (203 Czech Republic, guarantor, belonging to the institution), L. RICHTERA, L. VANICKOVA, V. ADAM and M. VACULOVICOVA

Edition

Journal of Dairy Science, New York, Elsevier Science Inc. 2020, 0022-0302

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

40101 Agriculture

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.034

RIV identification code

RIV/00216224:14740/20:00118333

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3168/jds.2019-17367

UT WoS

000536903900006

Keywords in English

biochanin A; genistein; dopamine; milk

Abstract

V originále

Dairy cow feed contains, among other ingredients, soybeans, legumes, and clover, plants that are rich in phytoestrogens. Several publications have reported a positive influence of phytoestrogens on human health; however, several unfavorable effects have also been reported. In this work, a simple, selective, and ecofriendly method of phytoestrogen isolation based on the technique of noncovalent molecular imprinting was developed. Genistein was used as a template, and dopamine was chosen as a functional monomer. A layer of molecularly imprinted polymers was created in a microtitration well plate. The binding capability and selective properties of obtained molecularly imprinted polymers were investigated. The imprinted polymers exhibited higher binding affinity toward chosen phytoestrogen than did the nonimprinted polymers. A selectivity factor of 6.94 was calculated, confirming satisfactory selectivity of the polymeric layer. The applicability of the proposed sensing method was tested by isolation of genistein from a real sample of bovine milk and combined with micellar electrokinetic capillary chromatography with UV-visible detection.

Links

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

90043, large research infrastructures

Name: CIISB

90127, large research infrastructures

Name: CIISB II

Citovat

BEZDEKOVA, J., M. VLCNOVSKA, K. ZEMANKOVA, R. BACOVA, M. KOLACKOVA, T. LEDNICKY, Jan PŘIBYL, L. RICHTERA, L. VANICKOVA, V. ADAM and M. VACULOVICOVA. Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk. Journal of Dairy Science. New York: Elsevier Science Inc., 2020, vol. 103, No 6, p. 4941-4950. ISSN 0022-0302. Available from: https://dx.doi.org/10.3168/jds.2019-17367.

@article{1749696,
   author = {Bezdekova, J. and Vlcnovska, M. and Zemankova, K. and Bacova, R. and Kolackova, M. and Lednicky, T. and Přibyl, Jan and Richtera, L. and Vanickova, L. and Adam, V. and Vaculovicova, M.},
   article_location = {New York},
   article_number = {6},
   doi = {http://dx.doi.org/10.3168/jds.2019-17367},
   keywords = {biochanin A; genistein; dopamine; milk},
   language = {eng},
   issn = {0022-0302},
   journal = {Journal of Dairy Science},
   title = {Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk},
   url = {https://www.sciencedirect.com/science/article/abs/pii/S0022030220302666?via%3Dihub},
   volume = {103},
   year = {2020}
}

TY  - JOUR
ID  - 1749696
AU  - Bezdekova, J. - Vlcnovska, M. - Zemankova, K. - Bacova, R. - Kolackova, M. - Lednicky, T. - Přibyl, Jan - Richtera, L. - Vanickova, L. - Adam, V. - Vaculovicova, M.
PY  - 2020
TI  - Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk
JF  - Journal of Dairy Science
VL  - 103
IS  - 6
SP  - 4941-4950
EP  - 4941-4950
PB  - Elsevier Science Inc.
SN  - 00220302
KW  - biochanin A
KW  - genistein
KW  - dopamine
KW  - milk
UR  - https://www.sciencedirect.com/science/article/abs/pii/S0022030220302666?via%3Dihub
N2  - Dairy cow feed contains, among other ingredients, soybeans, legumes, and clover, plants that are rich in phytoestrogens. Several publications have reported a positive influence of phytoestrogens on human health; however, several unfavorable effects have also been reported. In this work, a simple, selective, and ecofriendly method of phytoestrogen isolation based on the technique of noncovalent molecular imprinting was developed. Genistein was used as a template, and dopamine was chosen as a functional monomer. A layer of molecularly imprinted polymers was created in a microtitration well plate. The binding capability and selective properties of obtained molecularly imprinted polymers were investigated. The imprinted polymers exhibited higher binding affinity toward chosen phytoestrogen than did the nonimprinted polymers. A selectivity factor of 6.94 was calculated, confirming satisfactory selectivity of the polymeric layer. The applicability of the proposed sensing method was tested by isolation of genistein from a real sample of bovine milk and combined with micellar electrokinetic capillary chromatography with UV-visible detection.
ER  -

BEZDEKOVA, J., M. VLCNOVSKA, K. ZEMANKOVA, R. BACOVA, M. KOLACKOVA, T. LEDNICKY, Jan PŘIBYL, L. RICHTERA, L. VANICKOVA, V. ADAM and M. VACULOVICOVA. Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk. \textit{Journal of Dairy Science}. New York: Elsevier Science Inc., 2020, vol.~103, No~6, p.~4941-4950. ISSN~0022-0302. Available from: https://dx.doi.org/10.3168/jds.2019-17367.

Detailed Information on Publication Record