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@article{1749696, author = {Bezdekova, J. and Vlcnovska, M. and Zemankova, K. and Bacova, R. and Kolackova, M. and Lednicky, T. and Přibyl, Jan and Richtera, L. and Vanickova, L. and Adam, V. and Vaculovicova, M.}, article_location = {New York}, article_number = {6}, doi = {http://dx.doi.org/10.3168/jds.2019-17367}, keywords = {biochanin A; genistein; dopamine; milk}, language = {eng}, issn = {0022-0302}, journal = {Journal of Dairy Science}, title = {Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk}, url = {https://www.sciencedirect.com/science/article/abs/pii/S0022030220302666?via%3Dihub}, volume = {103}, year = {2020} }
TY - JOUR ID - 1749696 AU - Bezdekova, J. - Vlcnovska, M. - Zemankova, K. - Bacova, R. - Kolackova, M. - Lednicky, T. - Přibyl, Jan - Richtera, L. - Vanickova, L. - Adam, V. - Vaculovicova, M. PY - 2020 TI - Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk JF - Journal of Dairy Science VL - 103 IS - 6 SP - 4941-4950 EP - 4941-4950 PB - Elsevier Science Inc. SN - 00220302 KW - biochanin A KW - genistein KW - dopamine KW - milk UR - https://www.sciencedirect.com/science/article/abs/pii/S0022030220302666?via%3Dihub N2 - Dairy cow feed contains, among other ingredients, soybeans, legumes, and clover, plants that are rich in phytoestrogens. Several publications have reported a positive influence of phytoestrogens on human health; however, several unfavorable effects have also been reported. In this work, a simple, selective, and ecofriendly method of phytoestrogen isolation based on the technique of noncovalent molecular imprinting was developed. Genistein was used as a template, and dopamine was chosen as a functional monomer. A layer of molecularly imprinted polymers was created in a microtitration well plate. The binding capability and selective properties of obtained molecularly imprinted polymers were investigated. The imprinted polymers exhibited higher binding affinity toward chosen phytoestrogen than did the nonimprinted polymers. A selectivity factor of 6.94 was calculated, confirming satisfactory selectivity of the polymeric layer. The applicability of the proposed sensing method was tested by isolation of genistein from a real sample of bovine milk and combined with micellar electrokinetic capillary chromatography with UV-visible detection. ER -
BEZDEKOVA, J., M. VLCNOVSKA, K. ZEMANKOVA, R. BACOVA, M. KOLACKOVA, T. LEDNICKY, Jan PŘIBYL, L. RICHTERA, L. VANICKOVA, V. ADAM and M. VACULOVICOVA. Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk. \textit{Journal of Dairy Science}. New York: Elsevier Science Inc., 2020, vol.~103, No~6, p.~4941-4950. ISSN~0022-0302. Available from: https://dx.doi.org/10.3168/jds.2019-17367.
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