One-pot synthesis of natural amine-modified biocompatible
carbon quantum dots with antibacterial activity

J 2020

One-pot synthesis of natural amine-modified biocompatible carbon quantum dots with antibacterial activity

GAGIC, M., S. KOCIOVA, K. SMERKOVA, H. MICHALKOVA, M. SETKA et. al.

Basic information

Original name

One-pot synthesis of natural amine-modified biocompatible carbon quantum dots with antibacterial activity

Authors

GAGIC, M., S. KOCIOVA, K. SMERKOVA, H. MICHALKOVA, M. SETKA, P. SVEC, Jan PŘIBYL (203 Czech Republic, guarantor, belonging to the institution), J. MASILKO, R. BALKOVA, Z. HEGER, L. RICHTERA, V. ADAM and V. MILOSAVLJEVIC

Edition

Journal of Colloid and Interface Science, SAN DIEGO, Elsevier, 2020, 0021-9797

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10403 Physical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 8.128

RIV identification code

RIV/00216224:14740/20:00118337

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.jcis.2020.06.125

UT WoS

000581780400004

Keywords in English

Carbon quantum dots; Amines; Thermal decomposition; Toxicity; Antibacterial activity; ROS

Abstract

V originále

In the present study, the thermal decomposition of citric acid in the presence of biogenic amine was used to synthesize four different functionalized carbon quantum dots (CQDs), namely, histamine-(HCQDs), putrescine-(PCQDs), cadaverine-(CCQDs) and spermine-(SCQDs). The thermal decomposition of the precursors resulted in a decrease in stability and the formation of surface amides via a cross-linking process between the carboxyl and amine groups. The deposition of biogenic amines was confirmed by a structural characterization of the synthesized CQDs. The resulting CQDs, with a net zero charge, exhibited excellent stability in environments with different pH values. Through a set of different cytotoxicity tests, the absence of gene mutations, apoptosis, necrosis or disruption in cell membranes revealed the high biocompatibility of the CQDs. The antimicrobial activity of the synthesized CQDs was investigated against different bacterial species (Staphylococcus aureus, Escherichia coli, and Klebsiella pneumonia). We determined the growth kinetics, production of reactive oxygen species (ROS), cell viability and changes in membrane integrity by scanning electron microscopy (SEM). The minimal inhibitory concentrations (MICs) for S. aureus ranged from 3.4 to 6.9 mu g/mL. Regarding E. coli and K. pneumonia, all CQD formulations reduced growth, and the MICs were determined for CCQDs and HCQDs (6.9-19.4 mu g/mL). The antibacterial activity mechanism was attributed to the oxidative stress generated after CQD treatment, which resulted in the destabilization of the bacterial membrane. The bacterial permeability to propidium iodide indicated a change in membrane integrity, and the effect of CQDs on the morphology of the bacterial cells was evidenced by SEM. (C) 2020 Elsevier Inc. All rights reserved.

Links

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

90127, large research infrastructures

Name: CIISB II

Citovat

GAGIC, M., S. KOCIOVA, K. SMERKOVA, H. MICHALKOVA, M. SETKA, P. SVEC, Jan PŘIBYL, J. MASILKO, R. BALKOVA, Z. HEGER, L. RICHTERA, V. ADAM and V. MILOSAVLJEVIC. One-pot synthesis of natural amine-modified biocompatible carbon quantum dots with antibacterial activity. Journal of Colloid and Interface Science. SAN DIEGO: Elsevier, 2020, vol. 580, NOV, p. 30-48. ISSN 0021-9797. Available from: https://dx.doi.org/10.1016/j.jcis.2020.06.125.

@article{1749840,
   author = {Gagic, M. and Kociova, S. and Smerkova, K. and Michalkova, H. and Setka, M. and Svec, P. and Přibyl, Jan and Masilko, J. and Balkova, R. and Heger, Z. and Richtera, L. and Adam, V. and Milosavljevic, V.},
   article_location = {SAN DIEGO},
   article_number = {NOV},
   doi = {http://dx.doi.org/10.1016/j.jcis.2020.06.125},
   keywords = {Carbon quantum dots; Amines; Thermal decomposition; Toxicity; Antibacterial activity; ROS},
   language = {eng},
   issn = {0021-9797},
   journal = {Journal of Colloid and Interface Science},
   title = {One-pot synthesis of natural amine-modified biocompatible carbon quantum dots with antibacterial activity},
   url = {https://www.sciencedirect.com/science/article/pii/S0021979720308766?via%3Dihub},
   volume = {580},
   year = {2020}
}

TY  - JOUR
ID  - 1749840
AU  - Gagic, M. - Kociova, S. - Smerkova, K. - Michalkova, H. - Setka, M. - Svec, P. - Přibyl, Jan - Masilko, J. - Balkova, R. - Heger, Z. - Richtera, L. - Adam, V. - Milosavljevic, V.
PY  - 2020
TI  - One-pot synthesis of natural amine-modified biocompatible carbon quantum dots with antibacterial activity
JF  - Journal of Colloid and Interface Science
VL  - 580
IS  - NOV
SP  - 30-48
EP  - 30-48
PB  - Elsevier
SN  - 00219797
KW  - Carbon quantum dots
KW  - Amines
KW  - Thermal decomposition
KW  - Toxicity
KW  - Antibacterial activity
KW  - ROS
UR  - https://www.sciencedirect.com/science/article/pii/S0021979720308766?via%3Dihub
N2  - In the present study, the thermal decomposition of citric acid in the presence of biogenic amine was used to synthesize four different functionalized carbon quantum dots (CQDs), namely, histamine-(HCQDs), putrescine-(PCQDs), cadaverine-(CCQDs) and spermine-(SCQDs). The thermal decomposition of the precursors resulted in a decrease in stability and the formation of surface amides via a cross-linking process between the carboxyl and amine groups. The deposition of biogenic amines was confirmed by a structural characterization of the synthesized CQDs. The resulting CQDs, with a net zero charge, exhibited excellent stability in environments with different pH values. Through a set of different cytotoxicity tests, the absence of gene mutations, apoptosis, necrosis or disruption in cell membranes revealed the high biocompatibility of the CQDs. The antimicrobial activity of the synthesized CQDs was investigated against different bacterial species (Staphylococcus aureus, Escherichia coli, and Klebsiella pneumonia). We determined the growth kinetics, production of reactive oxygen species (ROS), cell viability and changes in membrane integrity by scanning electron microscopy (SEM). The minimal inhibitory concentrations (MICs) for S. aureus ranged from 3.4 to 6.9 mu g/mL. Regarding E. coli and K. pneumonia, all CQD formulations reduced growth, and the MICs were determined for CCQDs and HCQDs (6.9-19.4 mu g/mL). The antibacterial activity mechanism was attributed to the oxidative stress generated after CQD treatment, which resulted in the destabilization of the bacterial membrane. The bacterial permeability to propidium iodide indicated a change in membrane integrity, and the effect of CQDs on the morphology of the bacterial cells was evidenced by SEM. (C) 2020 Elsevier Inc. All rights reserved.
ER  -

GAGIC, M., S. KOCIOVA, K. SMERKOVA, H. MICHALKOVA, M. SETKA, P. SVEC, Jan PŘIBYL, J. MASILKO, R. BALKOVA, Z. HEGER, L. RICHTERA, V. ADAM and V. MILOSAVLJEVIC. One-pot synthesis of natural amine-modified biocompatible carbon quantum dots with antibacterial activity. \textit{Journal of Colloid and Interface Science}. SAN DIEGO: Elsevier, 2020, vol.~580, NOV, p.~30-48. ISSN~0021-9797. Available from: https://dx.doi.org/10.1016/j.jcis.2020.06.125.

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