| KOTOUCEK, J., F. HUBATKA, J. MASEK, P. KULICH, K. VELINSKA, J. BEZDEKOVA, M. FOJTIKOVA, E. BARTHELDYOVA, A. TOMECKOVA, J. STRASKA, Dominik HREBÍK, S. MACAULAY, I. KRATOCHVILOVA, M. RASKA and J. TURANEK. Preparation of nanoliposomes by microfluidic mixing in herring-bone channel and the role of membrane fluidity in liposomes formation. Nature Scientific Reports. London: NATURE PUBLISHING GROUP, 2020, vol. 10, No 1, p. 5595-5605. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-020-62500-2. |
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@article{1749860,
author = {Kotoucek, J. and Hubatka, F. and Masek, J. and Kulich, P. and Velinska, K. and Bezdekova, J. and Fojtikova, M. and Bartheldyova, E. and Tomeckova, A. and Straska, J. and Hrebík, Dominik and Macaulay, S. and Kratochvilova, I. and Raska, M. and Turanek, J.},
article_location = {London},
article_number = {1},
doi = {http://dx.doi.org/10.1038/s41598-020-62500-2},
keywords = {SIZE DISTRIBUTION; DIPHENYLHEXATRIENE; NANOPARTICLES; SYSTEMS},
language = {eng},
issn = {2045-2322},
journal = {Nature Scientific Reports},
title = {Preparation of nanoliposomes by microfluidic mixing in herring-bone channel and the role of membrane fluidity in liposomes formation},
url = {https://www.nature.com/articles/s41598-020-62500-2},
volume = {10},
year = {2020}
}
TY - JOUR
ID - 1749860
AU - Kotoucek, J. - Hubatka, F. - Masek, J. - Kulich, P. - Velinska, K. - Bezdekova, J. - Fojtikova, M. - Bartheldyova, E. - Tomeckova, A. - Straska, J. - Hrebík, Dominik - Macaulay, S. - Kratochvilova, I. - Raska, M. - Turanek, J.
PY - 2020
TI - Preparation of nanoliposomes by microfluidic mixing in herring-bone channel and the role of membrane fluidity in liposomes formation
JF - Nature Scientific Reports
VL - 10
IS - 1
SP - 5595
EP - 5595
PB - NATURE PUBLISHING GROUP
SN - 20452322
KW - SIZE DISTRIBUTION
KW - DIPHENYLHEXATRIENE
KW - NANOPARTICLES
KW - SYSTEMS
UR - https://www.nature.com/articles/s41598-020-62500-2
N2 - Introduction of microfluidic mixing technique opens a new door for preparation of the liposomes and lipid-based nanoparticles by on-chip technologies that are applicable in a laboratory and industrial scale. This study demonstrates the role of phospholipid bilayer fragment as the key intermediate in the mechanism of liposome formation by microfluidic mixing in the channel with "herring-bone" geometry used with the instrument NanoAssemblr. The fluidity of the lipid bilayer expressed as fluorescence anisotropy of the probe N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl) was found to be the basic parameter affecting the final size of formed liposomes prepared by microfluidic mixing of an ethanol solution of lipids and water phase. Both saturated and unsaturated lipids together with various content of cholesterol were used for liposome preparation and it was demonstrated, that an increase in fluidity results in a decrease of liposome size as analyzed by DLS. Gadolinium chelating lipids were used to visualize the fine structure of liposomes and bilayer fragments by CryoTEM. Experimental data and theoretical calculations are in good accordance with the theory of lipid disc micelle vesiculation.
ER -
KOTOUCEK, J., F. HUBATKA, J. MASEK, P. KULICH, K. VELINSKA, J. BEZDEKOVA, M. FOJTIKOVA, E. BARTHELDYOVA, A. TOMECKOVA, J. STRASKA, Dominik HREBÍK, S. MACAULAY, I. KRATOCHVILOVA, M. RASKA and J. TURANEK. Preparation of nanoliposomes by microfluidic mixing in herring-bone channel and the role of membrane fluidity in liposomes formation. \textit{Nature Scientific Reports}. London: NATURE PUBLISHING GROUP, 2020, vol.~10, No~1, p.~5595-5605. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-020-62500-2.
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