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@article{1750438,
author = {Vozdova, M. and Kubickova, S. and Pál, Karol and Frohlich, J. and Fictum, P. and Rubes, J.},
article_location = {HOBOKEN},
article_number = {4},
doi = {http://dx.doi.org/10.1111/vco.12572},
keywords = {cancer; dog; GNB1; KIT; mast cell tumour; mutation; next generation sequencing; whole exome sequencing},
language = {eng},
issn = {1476-5810},
journal = {VETERINARY AND COMPARATIVE ONCOLOGY},
title = {Recurrent gene mutations detected in canine mast cell tumours by next generation sequencing},
url = {https://onlinelibrary.wiley.com/doi/10.1111/vco.12572},
volume = {18},
year = {2020}
}
TY - JOUR
ID - 1750438
AU - Vozdova, M. - Kubickova, S. - Pál, Karol - Frohlich, J. - Fictum, P. - Rubes, J.
PY - 2020
TI - Recurrent gene mutations detected in canine mast cell tumours by next generation sequencing
JF - VETERINARY AND COMPARATIVE ONCOLOGY
VL - 18
IS - 4
SP - 509-518
EP - 509-518
PB - WILEY
SN - 14765810
KW - cancer
KW - dog
KW - GNB1
KW - KIT
KW - mast cell tumour
KW - mutation
KW - next generation sequencing
KW - whole exome sequencing
UR - https://onlinelibrary.wiley.com/doi/10.1111/vco.12572
N2 - Genetic causes of canine mast cell tumours (MCTs), except for mutations in the KIT gene detected in some MCTs, are generally unknown. We used whole exome sequencing to reveal mutation spectra in canine MCTs. We detected somatic mutations in 87 genes including 10 genes recognized as human cancer drivers. Besides KIT, 14 other genes were recurrently mutated. Subsequently, we performed next generation sequencing of a panel of 50 selected genes in additional MCT samples. In this group, the most frequently altered gene was GNB1 showing a recurrent dinucleotide substitution at position of Gly116 in 30% of the MCT samples (n = 6/20) and Ile80 substitution accompanied by a splice region mutation in one case. We extended the study by analysis of the above mentioned GNB1 regions in additional MCT samples by Sanger sequencing, and assessed the overall prevalence of GNB1 mutations to 17.3% (n = 14/81), which is similar to the prevalence of KIT alterations. Our results indicate that GNB1 mutations are probably involved in canine MCT pathogenesis in both cutaneous and subcutaneous MCT cases. As opposed to KIT alterations, the presence of GNB1 mutations did not negatively affect survival times, and our data even showed a trend towards positive prognosis. If our results are confirmed in a larger number of MCTs, an extension of molecular testing of canine MCTs by GNB1 analysis would help to refine the molecular stratification of MCTs, and become useful for targeted treatment strategies.
ER -
VOZDOVA, M., S. KUBICKOVA, Karol PÁL, J. FROHLICH, P. FICTUM and J. RUBES. Recurrent gene mutations detected in canine mast cell tumours by next generation sequencing. \textit{VETERINARY AND COMPARATIVE ONCOLOGY}. HOBOKEN: WILEY, 2020, vol.~18, No~4, p.~509-518. ISSN~1476-5810. Available from: https://dx.doi.org/10.1111/vco.12572.
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