The Effect of Synthesis Procedure on Hydrogen Peroxidase-Like Catalytic Activity of Iron Oxide Magnetic Particles
Authors
MUKHERJEE, A., A.M. ASHRAFI, P. SVEC, L. RICHTERA, Jan PŘIBYL (203 Czech Republic, guarantor, belonging to the institution), M. BRTNICKY, J. KYNICKY and V. ADAM
A comparative study was carried out using magnetic nanoparticles (MNPs) for the fabrication of non-enzymatic sensors for the continuous and rapid detection and monitoring of H2O2. Various MNPs, differing in terms of their synthesis procedure and modification, were synthesized and characterized by different techniques. The electrochemical catalytic activity of the synthesized MNPs toward the reduction in H2O2 was investigated by cyclic voltammetry. The naked MNPs showed the highest catalytic activity among all the synthesized MNPs. The biosensor based on the naked MNPs was then applied in the determination of H2O2 using chronoamperometry. The parameters such as the applied cathodic potential and the amount of MNPs on the developed biosensor were optimized. Moreover, the analytical figures of merit, including reproducibility (RSD = 6.14%), sensitivity (m = 0.0676 mu A mu M-1), limit of detection (LOD) = 27.02 mu mol L-1, and limit of quantification (LOQ) = 89.26 mu mol L-1 of the developed biosensor indicate satisfactory analysis. Finally, MNPs were successfully utilized for the determination of H2O2 in milk.
Links
LM2018127, research and development project
Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)
Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR