Polymethacrylate monolithic columns for capillary liquid
chromatography

J 2008

Polymethacrylate monolithic columns for capillary liquid chromatography

URBAN, Jiří and P JANDERA

Basic information

Original name

Polymethacrylate monolithic columns for capillary liquid chromatography

Authors

URBAN, Jiří and P JANDERA

Edition

Journal of separation science, Weinheim, WILEY-V C H VERLAG GMBH, 2008, 1615-9306

Other information

Language

English

Type of outcome

Article in a journal

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 2.746

DOI

https://doi.org/10.1002/jssc.200800182

UT WoS

000258668000002

Keywords in English

capillary HPLC; monolithic columns; polymethacrylate stationary phases; pore structure

Changed: 12/3/2021 13:21, doc. RNDr. Jiří Urban, Ph.D.

Abstract

In the original language

In recent years, continuous separation media have attracted considerable attention because of the advantages they offer over packed columns. This research resulted in two useful monolithic material types, the first based on modified silica gel and the second on organic polymers. This work attempts to review advances in the development, characterization, and applications of monolithic columns based on synthetic polymers in capillary chromatography, with the main focus on monolithic beds prepared from methacrylate-ester based monomers. The polymerization conditions used in the production of polymethacrylate monolithic capillary columns are surveyed, with attention being paid to the concentrations of monomers, porogen solvents, and polymerization initiators as the system variables used to control the porous and hydrodynamic properties of the monolithic media. The simplicity of their preparation as well as the possibilities of controlling of their porous properties and surface chemistries are the main benefits of the polymer monolithic capillary columns in comparison to capillary columns packed with particulate materials. The application areas considered in this review concern mainly separations in reversed-phase chromatography, ion-exchange chromatography, hydrophobic and hydrophilic interaction modes: enzyme immobilization and sample preparation in the capillary chromatography format are also addressed.

Cite

URBAN, Jiří and P JANDERA. Polymethacrylate monolithic columns for capillary liquid chromatography. Journal of separation science. Weinheim: WILEY-V C H VERLAG GMBH, 2008, vol. 31, No 14, p. 2521-2540. ISSN 1615-9306. Available from: https://doi.org/10.1002/jssc.200800182.

@article{1751683,
   author = {Urban, Jiří and Jandera, P},
   article_location = {Weinheim},
   article_number = {14},
   doi = {https://doi.org/10.1002/jssc.200800182},
   keywords = {capillary HPLC; monolithic columns; polymethacrylate stationary phases; pore structure},
   language = {eng},
   issn = {1615-9306},
   journal = {Journal of separation science},
   title = {Polymethacrylate monolithic columns for capillary liquid chromatography},
   volume = {31},
   year = {2008}
}

TY  - JOUR
ID  - 1751683
AU  - Urban, Jiří - Jandera, P
PY  - 2008
TI  - Polymethacrylate monolithic columns for capillary liquid chromatography
JF  - Journal of separation science
VL  - 31
IS  - 14
SP  - 2521-2540
EP  - 2521-2540
PB  - WILEY-V C H VERLAG GMBH
SN  - 16159306
KW  - capillary HPLC
KW  - monolithic columns
KW  - polymethacrylate stationary phases
KW  - pore structure
N2  - In recent years, continuous separation media have attracted considerable attention because of the advantages they offer over packed columns. This research resulted in two useful monolithic material types, the first based on modified silica gel and the second on organic polymers. This work attempts to review advances in the development, characterization, and applications of monolithic columns based on synthetic polymers in capillary chromatography, with the main focus on monolithic beds prepared from methacrylate-ester based monomers. The polymerization conditions used in the production of polymethacrylate monolithic capillary columns are surveyed, with attention being paid to the concentrations of monomers, porogen solvents, and polymerization initiators as the system variables used to control the porous and hydrodynamic properties of the monolithic media. The simplicity of their preparation as well as the possibilities of controlling of their porous properties and surface chemistries are the main benefits of the polymer monolithic capillary columns in comparison to capillary columns packed with particulate materials. The application areas considered in this review concern mainly separations in reversed-phase chromatography, ion-exchange chromatography, hydrophobic and hydrophilic interaction modes: enzyme immobilization and sample preparation in the capillary chromatography format are also addressed.
ER  -

URBAN, Jiří and P JANDERA. Polymethacrylate monolithic columns for capillary liquid chromatography. \textit{Journal of separation science}. Weinheim: WILEY-V C H VERLAG GMBH, 2008, vol.~31, No~14, p.~2521-2540. ISSN~1615-9306. Available from: https://doi.org/10.1002/jssc.200800182.

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