Bacterial Diversity in the Asphalt Concrete Lining of the Upper
Water Reservoir of a Pumped-Storage Scheme

J 2020

Bacterial Diversity in the Asphalt Concrete Lining of the Upper Water Reservoir of a Pumped-Storage Scheme

SPANO, M., J. RIHA, A. SPANOVA, Ondrej ŠEDO, B. RITTICH et. al.

Basic information

Original name

Bacterial Diversity in the Asphalt Concrete Lining of the Upper Water Reservoir of a Pumped-Storage Scheme

Authors

SPANO, M., J. RIHA, A. SPANOVA, Ondrej ŠEDO (203 Czech Republic, guarantor, belonging to the institution) and B. RITTICH

Edition

WATER, London, Proprietors, the Colliery Guardian Company, 2020, 2073-4441

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10503 Water resources

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.103

RIV identification code

RIV/00216224:14740/20:00118387

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3390/w12113045

UT WoS

000594873200001

Keywords in English

pumped-storage hydropower plant; asphalt concrete lining; open blisters; bacterial diversity; MALDI-TOF MS

Abstract

V originále

Asphalt concrete linings (ACLs) are frequently used in pumped-storage hydropower schemes with frequently oscillating reservoir water levels. ACLs can be damaged during operation, which shortens their service life. One type of damage is the formation of blisters. In this study, the bacterial diversity in damaged and undamaged portions of the ACL of an upper water reservoir was evaluated. Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) was employed for the identification of the bacterial strains. No bacteria were detected growing inside the undamaged dense layer. Low bacterial diversity (2 and 4 morphotypes) was found in the upper part that has no contact with the reservoir water and in the lowest part that is permanently under water, respectively. More complex bacterial diversity (16 morphotypes covered by 2 different genera and 4 species) was found on the mastic coating of the ACL, which was alternately under and above the water. Very complex bacterial compositions (53 morphotypes covered by at least 13 different species from 6 genera) were found in samples taken from open blisters. Bacterial genera that are known to utilize asphalt products as a source of nutrition were found in the open blisters. The results reflect the importance of bacterial diversity in blisters and their possible role in ACL degradation.

Links

EF16_013/0001776, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii pro lidské zdraví

90127, large research infrastructures

Name: CIISB II

Citovat

SPANO, M., J. RIHA, A. SPANOVA, Ondrej ŠEDO and B. RITTICH. Bacterial Diversity in the Asphalt Concrete Lining of the Upper Water Reservoir of a Pumped-Storage Scheme. WATER. London: Proprietors, the Colliery Guardian Company, 2020, vol. 12, No 11, p. 3045-3059. ISSN 2073-4441. Available from: https://dx.doi.org/10.3390/w12113045.

@article{1752477,
   author = {Spano, M. and Riha, J. and Spanova, A. and Šedo, Ondrej and Rittich, B.},
   article_location = {London},
   article_number = {11},
   doi = {http://dx.doi.org/10.3390/w12113045},
   keywords = {pumped-storage hydropower plant; asphalt concrete lining; open blisters; bacterial diversity; MALDI-TOF MS},
   language = {eng},
   issn = {2073-4441},
   journal = {WATER},
   title = {Bacterial Diversity in the Asphalt Concrete Lining of the Upper Water Reservoir of a Pumped-Storage Scheme},
   url = {https://www.mdpi.com/2073-4441/12/11/3045},
   volume = {12},
   year = {2020}
}

TY  - JOUR
ID  - 1752477
AU  - Spano, M. - Riha, J. - Spanova, A. - Šedo, Ondrej - Rittich, B.
PY  - 2020
TI  - Bacterial Diversity in the Asphalt Concrete Lining of the Upper Water Reservoir of a Pumped-Storage Scheme
JF  - WATER
VL  - 12
IS  - 11
SP  - 3045
EP  - 3045
PB  - Proprietors, the Colliery Guardian Company
SN  - 20734441
KW  - pumped-storage hydropower plant
KW  - asphalt concrete lining
KW  - open blisters
KW  - bacterial diversity
KW  - MALDI-TOF MS
UR  - https://www.mdpi.com/2073-4441/12/11/3045
N2  - Asphalt concrete linings (ACLs) are frequently used in pumped-storage hydropower schemes with frequently oscillating reservoir water levels. ACLs can be damaged during operation, which shortens their service life. One type of damage is the formation of blisters. In this study, the bacterial diversity in damaged and undamaged portions of the ACL of an upper water reservoir was evaluated. Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) was employed for the identification of the bacterial strains. No bacteria were detected growing inside the undamaged dense layer. Low bacterial diversity (2 and 4 morphotypes) was found in the upper part that has no contact with the reservoir water and in the lowest part that is permanently under water, respectively. More complex bacterial diversity (16 morphotypes covered by 2 different genera and 4 species) was found on the mastic coating of the ACL, which was alternately under and above the water. Very complex bacterial compositions (53 morphotypes covered by at least 13 different species from 6 genera) were found in samples taken from open blisters. Bacterial genera that are known to utilize asphalt products as a source of nutrition were found in the open blisters. The results reflect the importance of bacterial diversity in blisters and their possible role in ACL degradation.
ER  -

SPANO, M., J. RIHA, A. SPANOVA, Ondrej ŠEDO and B. RITTICH. Bacterial Diversity in the Asphalt Concrete Lining of the Upper Water Reservoir of a Pumped-Storage Scheme. \textit{WATER}. London: Proprietors, the Colliery Guardian Company, 2020, vol.~12, No~11, p.~3045-3059. ISSN~2073-4441. Available from: https://dx.doi.org/10.3390/w12113045.

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