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@inproceedings{1752518, author = {Přibyl, Jan and Rotrekl, Vladimír and Pešl, Martin and Jelínková, Šárka and Kratochvílová, Irena}, address = {Brno}, booktitle = {Nanocon 2020}, doi = {http://dx.doi.org/10.37904/nanocon.2020.3740}, edition = {2020}, keywords = {Atomic Force Microscopy; Mechanical Mapping; Cell stiffness; Cryopreservation}, howpublished = {elektronická verze "online"}, language = {eng}, location = {Brno}, pages = {1-6}, publisher = {TANGER Ltd.}, title = {TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES}, url = {https://www.confer.cz/nanocon/2020/3740-time-lapse-monitoring-of-cell-mechanical-properties}, year = {2020} }
TY - JOUR ID - 1752518 AU - Přibyl, Jan - Rotrekl, Vladimír - Pešl, Martin - Jelínková, Šárka - Kratochvílová, Irena PY - 2020 TI - TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES PB - TANGER Ltd. CY - Brno KW - Atomic Force Microscopy KW - Mechanical Mapping KW - Cell stiffness KW - Cryopreservation UR - https://www.confer.cz/nanocon/2020/3740-time-lapse-monitoring-of-cell-mechanical-properties N2 - Atomic force microscopy (AFM) is a highly sensitive non-invasive surface method able to provide insight into cells' mechanical parameters. Membrane and sub-membrane development, as well as internal cellular properties, can be monitored. The stiffness of cells is a fundamental phenomenon that reflects changes in cell physiology. More importantly, changes in cell mechanical properties are also often found to be closely associated with various disease conditions. Cell mechanics are mainly dependent on cytoskeletal architecture. The development of cryopreserved cells' mechanical properties (stiffness) after thawing was studied using AFM. Cell stiffness was mapped and thus monitored in time and space under nearly physiological conditions (i.e., in culture medium and at elevated temperature). In AFM force spectroscopy mode, cells are indented at many sites, and their complete elastic responses are recorded, enabling them to reconstruct a stiffness map. We measured the frozen cell surface stiffness immediately after thawing; they, when the dynamics of development of the cell stiffness were monitored in time up to 24 hours. Moreover, the AFM spectroscopy was combined with fluorescence-based staining of the cytoskeleton, thus enabling to directly correlate cytoskeleton development with stiffness mapping ER -
PŘIBYL, Jan, Vladimír ROTREKL, Martin PEŠL, Šárka JELÍNKOVÁ and Irena KRATOCHVÍLOVÁ. TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES. Online. In \textit{Nanocon 2020}. 2020th ed. Brno: TANGER Ltd., 2020, p.~1-6. ISSN~2694-930X. Available from: https://dx.doi.org/10.37904/nanocon.2020.3740.
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