2020
Structural Characterization of the Interaction Between BRCA1-BARD1 and RNA Polymerase II
KLÁPŠŤOVÁ, Veronika; Marek ŠEBESTA; Jiří NOVÁČEK a Richard ŠTEFLZákladní údaje
Originální název
Structural Characterization of the Interaction Between BRCA1-BARD1 and RNA Polymerase II
Autoři
Vydání
CEITEC PhD Conference, 2020
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10608 Biochemistry and molecular biology
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14740/20:00118408
Organizační jednotka
Středoevropský technologický institut
Klíčová slova anglicky
BRCA1-BARD1; RNA Polymerase II
Štítky
Změněno: 17. 3. 2021 14:42, Mgr. Pavla Foltynová, Ph.D.
Anotace
V originále
Transcrption is considered one of the major threats for genome stability; as the conflicts of the transcription machinery with the replication fork, or another barrier, can lead to double-stranded DNA breaks. Although maintaining genome integrity is crucial for the cell viability, the mechanisms responsible for avoiding these conflicts are poorly characterized. Therefore, my research project focuses on structural characterization of the interaction between RNA polymerase II (RNAPII) and BRCA1-BARD1 complex, one of possible players involved in maintaining the genome stability. Recently, we have confirmed the interaction between the C-terminal domain of RNAPII and the BRCT domains of BRCA1 and BARD1, respectively, and we are reconstituting the full-length complex for cryoelectron microscopy studies. Structural characterization of the complex, as well as description of the conditions under which it is formed will help us to analyse its function in preventing transcription-borne DNA damage. This, in turn, will help us to understand how cells coordinate transcription and other competing processes on DNA, such as replication or DNA repair.
Návaznosti
| LQ1601, projekt VaV |
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