Structural Characterization of the Interaction Between BRCA1-BARD1 and RNA Polymerase II

KLÁPŠŤOVÁ, Veronika, Marek ŠEBESTA, Jiří NOVÁČEK and Richard ŠTEFL. Structural Characterization of the Interaction Between BRCA1-BARD1 and RNA Polymerase II. In CEITEC PhD Conference. 2020.

Basic information

• Original name: Structural Characterization of the Interaction Between BRCA1-BARD1 and RNA Polymerase II
• Authors: KLÁPŠŤOVÁ, Veronika (203 Czech Republic, belonging to the institution), Marek ŠEBESTA (703 Slovakia, belonging to the institution), Jiří NOVÁČEK (203 Czech Republic, belonging to the institution) and Richard ŠTEFL (203 Czech Republic, belonging to the institution).
• Edition: CEITEC PhD Conference, 2020.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14740/20:00118408
• Organization unit: Central European Institute of Technology
• Keywords in English: BRCA1-BARD1; RNA Polymerase II
• Tags: rivok

Abstract

Transcrption is considered one of the major threats for genome stability; as the conflicts of the transcription machinery with the replication fork, or another barrier, can lead to double-stranded DNA breaks. Although maintaining genome integrity is crucial for the cell viability, the mechanisms responsible for avoiding these conflicts are poorly characterized. Therefore, my research project focuses on structural characterization of the interaction between RNA polymerase II (RNAPII) and BRCA1-BARD1 complex, one of possible players involved in maintaining the genome stability. Recently, we have confirmed the interaction between the C-terminal domain of RNAPII and the BRCT domains of BRCA1 and BARD1, respectively, and we are reconstituting the full-length complex for cryoelectron microscopy studies. Structural characterization of the complex, as well as description of the conditions under which it is formed will help us to analyse its function in preventing transcription-borne DNA damage. This, in turn, will help us to understand how cells coordinate transcription and other competing processes on DNA, such as replication or DNA repair.

Links

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR