a 2020

Structural Characterization of the Interaction Between BRCA1-BARD1 and RNA Polymerase II

KLÁPŠŤOVÁ, Veronika, Marek ŠEBESTA, Jiří NOVÁČEK and Richard ŠTEFL

Basic information

Original name

Structural Characterization of the Interaction Between BRCA1-BARD1 and RNA Polymerase II

Authors

KLÁPŠŤOVÁ, Veronika (203 Czech Republic, belonging to the institution), Marek ŠEBESTA (703 Slovakia, belonging to the institution), Jiří NOVÁČEK (203 Czech Republic, belonging to the institution) and Richard ŠTEFL (203 Czech Republic, belonging to the institution)

Edition

CEITEC PhD Conference, 2020

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

RIV identification code

RIV/00216224:14740/20:00118408

Organization unit

Central European Institute of Technology

Keywords in English

BRCA1-BARD1; RNA Polymerase II

Tags

Změněno: 17/3/2021 14:42, Mgr. Pavla Foltynová, Ph.D.

Abstract

V originále

Transcrption is considered one of the major threats for genome stability; as the conflicts of the transcription machinery with the replication fork, or another barrier, can lead to double-stranded DNA breaks. Although maintaining genome integrity is crucial for the cell viability, the mechanisms responsible for avoiding these conflicts are poorly characterized. Therefore, my research project focuses on structural characterization of the interaction between RNA polymerase II (RNAPII) and BRCA1-BARD1 complex, one of possible players involved in maintaining the genome stability. Recently, we have confirmed the interaction between the C-terminal domain of RNAPII and the BRCT domains of BRCA1 and BARD1, respectively, and we are reconstituting the full-length complex for cryoelectron microscopy studies. Structural characterization of the complex, as well as description of the conditions under which it is formed will help us to analyse its function in preventing transcription-borne DNA damage. This, in turn, will help us to understand how cells coordinate transcription and other competing processes on DNA, such as replication or DNA repair.

Links

LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR