Dual Effect of Taxifolin on ZEB2 Cancer Signaling in HepG2
Cells

J 2021

Dual Effect of Taxifolin on ZEB2 Cancer Signaling in HepG2 Cells

DOSTÁL, Zdeněk, Martin SEBERA, Josef SROVNAL, Kateřina ŠTAFFOVÁ, Martin MODRIANSKÝ et. al.

Basic information

Original name

Dual Effect of Taxifolin on ZEB2 Cancer Signaling in HepG2 Cells

Authors

DOSTÁL, Zdeněk, Martin SEBERA (203 Czech Republic, guarantor, belonging to the institution), Josef SROVNAL, Kateřina ŠTAFFOVÁ and Martin MODRIANSKÝ

Edition

Molecules, Mayer und Muller, 2021, 1420-3049

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30306 Sport and fitness sciences

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.927

RIV identification code

RIV/00216224:14510/21:00121251

Organization unit

Faculty of Sports Studies

DOI

http://dx.doi.org/10.3390/molecules26051476

UT WoS

000628430300001

Keywords in English

polyphenols; Affymetrix GeneChip™ miRNA 3.0 Array; Hep G2 cells; primary cultures of human hepatocytes; ZEB2

Abstract

V originále

Polyphenols, secondary metabolites of plants, exhibit different anti-cancer and cytoprotective properties such as anti-radical, anti-angiogenic, anti-inflammation, or cardioprotective. Some of these activities could be linked to modulation of miRNAs expression. MiRNAs play an important role in posttranscriptional regulation of their target genes that could be important within cell signalling or preservation of cell homeostasis, e.g., cell survival/apoptosis. We evaluated the influence of a non-toxic concentration of taxifolin and quercetin on the expression of majority human miRNAs via Affymetrix GeneChip™ miRNA 3.0 Array. For the evaluation we used two cell models corresponding to liver tissue, Hep G2 and primary human hepatocytes. The array analysis identified four miRNAs, miR-153, miR-204, miR-211, and miR-377-3p, with reduced expression after taxifolin treatment. All of these miRNAs are linked to modulation of ZEB2 expression in various models. Indeed, ZEB2 protein displayed upregulation after taxifolin treatment in a dose dependent manner. However, the modulation did not lead to epithelial mesenchymal transition. Our data show that taxifolin inhibits Akt phosphorylation, thereby diminishing ZEB2 signalling that could trigger carcinogenesis. We conclude that biological activity of taxifolin may have ambiguous or even contradictory outcomes because of non-specific effect on the cell.

Citovat

DOSTÁL, Zdeněk, Martin SEBERA, Josef SROVNAL, Kateřina ŠTAFFOVÁ and Martin MODRIANSKÝ. Dual Effect of Taxifolin on ZEB2 Cancer Signaling in HepG2 Cells. Molecules. Mayer und Muller, 2021, vol. 26, No 5, p. nestránkováno, 20 pp. ISSN 1420-3049. Available from: https://dx.doi.org/10.3390/molecules26051476.

@article{1753097,
   author = {Dostál, Zdeněk and Sebera, Martin and Srovnal, Josef and Štaffová, Kateřina and Modrianský, Martin},
   article_number = {5},
   doi = {http://dx.doi.org/10.3390/molecules26051476},
   keywords = {polyphenols; Affymetrix GeneChip™ miRNA 3.0 Array; Hep G2 cells; primary cultures of human hepatocytes; ZEB2},
   language = {eng},
   issn = {1420-3049},
   journal = {Molecules},
   title = {Dual Effect of Taxifolin on ZEB2 Cancer Signaling in HepG2 Cells},
   url = {https://www.mdpi.com/1420-3049/26/5/1476},
   volume = {26},
   year = {2021}
}

TY  - JOUR
ID  - 1753097
AU  - Dostál, Zdeněk - Sebera, Martin - Srovnal, Josef - Štaffová, Kateřina - Modrianský, Martin
PY  - 2021
TI  - Dual Effect of Taxifolin on ZEB2 Cancer Signaling in HepG2 Cells
JF  - Molecules
VL  - 26
IS  - 5
SP  - nestránkováno
EP  - nestránkováno
PB  - Mayer und Muller
SN  - 14203049
KW  - polyphenols
KW  - Affymetrix GeneChip™ miRNA 3.0 Array
KW  - Hep G2 cells
KW  - primary cultures of human hepatocytes
KW  - ZEB2
UR  - https://www.mdpi.com/1420-3049/26/5/1476
N2  - Polyphenols, secondary metabolites of plants, exhibit different anti-cancer and cytoprotective properties such as anti-radical, anti-angiogenic, anti-inflammation, or cardioprotective. Some of these activities could be linked to modulation of miRNAs expression. MiRNAs play an important role in posttranscriptional regulation of their target genes that could be important within cell signalling or preservation of cell homeostasis, e.g., cell survival/apoptosis. We evaluated the influence of a non-toxic concentration of taxifolin and quercetin on the expression of majority human miRNAs via Affymetrix GeneChip™ miRNA 3.0 Array. For the evaluation we used two cell models corresponding to liver tissue, Hep G2 and primary human hepatocytes. The array analysis identified four miRNAs, miR-153, miR-204, miR-211, and miR-377-3p, with reduced expression after taxifolin treatment. All of these miRNAs are linked to modulation of ZEB2 expression in various models. Indeed, ZEB2 protein displayed upregulation after taxifolin treatment in a dose dependent manner. However, the modulation did not lead to epithelial mesenchymal transition. Our data show that taxifolin inhibits Akt phosphorylation, thereby diminishing ZEB2 signalling that could trigger carcinogenesis. We conclude that biological activity of taxifolin may have ambiguous or even contradictory outcomes because of non-specific effect on the cell.
ER  -

DOSTÁL, Zdeněk, Martin SEBERA, Josef SROVNAL, Kateřina ŠTAFFOVÁ and Martin MODRIANSKÝ. Dual Effect of Taxifolin on ZEB2 Cancer Signaling in HepG2 Cells. \textit{Molecules}. Mayer und Muller, 2021, vol.~26, No~5, p.~nestránkováno, 20 pp. ISSN~1420-3049. Available from: https://dx.doi.org/10.3390/molecules26051476.

Detailed Information on Publication Record