Dual Effect of Taxifolin on ZEB2 Cancer Signaling in HepG2 Cells

DOSTÁL, Zdeněk, Martin SEBERA, Josef SROVNAL, Kateřina ŠTAFFOVÁ and Martin MODRIANSKÝ. Dual Effect of Taxifolin on ZEB2 Cancer Signaling in HepG2 Cells. Molecules. Mayer und Muller, 2021, vol. 26, No 5, p. nestránkováno, 20 pp. ISSN 1420-3049. Available from: https://dx.doi.org/10.3390/molecules26051476.

Basic information

• Original name: Dual Effect of Taxifolin on ZEB2 Cancer Signaling in HepG2 Cells
• Authors: DOSTÁL, Zdeněk, Martin SEBERA (203 Czech Republic, guarantor, belonging to the institution), Josef SROVNAL, Kateřina ŠTAFFOVÁ and Martin MODRIANSKÝ.
• Edition: Molecules, Mayer und Muller, 2021, 1420-3049.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30306 Sport and fitness sciences
• Country of publisher: Switzerland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 4.927
• RIV identification code: RIV/00216224:14510/21:00121251
• Organization unit: Faculty of Sports Studies
• Doi: http://dx.doi.org/10.3390/molecules26051476
• UT WoS: 000628430300001
• Keywords in English: polyphenols; Affymetrix GeneChip™ miRNA 3.0 Array; Hep G2 cells; primary cultures of human hepatocytes; ZEB2
• Tags: International impact, Reviewed

Abstract

Polyphenols, secondary metabolites of plants, exhibit different anti-cancer and cytoprotective properties such as anti-radical, anti-angiogenic, anti-inflammation, or cardioprotective. Some of these activities could be linked to modulation of miRNAs expression. MiRNAs play an important role in posttranscriptional regulation of their target genes that could be important within cell signalling or preservation of cell homeostasis, e.g., cell survival/apoptosis. We evaluated the influence of a non-toxic concentration of taxifolin and quercetin on the expression of majority human miRNAs via Affymetrix GeneChip™ miRNA 3.0 Array. For the evaluation we used two cell models corresponding to liver tissue, Hep G2 and primary human hepatocytes. The array analysis identified four miRNAs, miR-153, miR-204, miR-211, and miR-377-3p, with reduced expression after taxifolin treatment. All of these miRNAs are linked to modulation of ZEB2 expression in various models. Indeed, ZEB2 protein displayed upregulation after taxifolin treatment in a dose dependent manner. However, the modulation did not lead to epithelial mesenchymal transition. Our data show that taxifolin inhibits Akt phosphorylation, thereby diminishing ZEB2 signalling that could trigger carcinogenesis. We conclude that biological activity of taxifolin may have ambiguous or even contradictory outcomes because of non-specific effect on the cell.