Changes in haemolymph parameters and insect ability to respond
to immune challenge during overwintering

J 2021

Changes in haemolymph parameters and insect ability to respond to immune challenge during overwintering

ŘEŘICHA, Michal, Pavel DOBEŠ and Michal KNAPP

Basic information

Original name

Changes in haemolymph parameters and insect ability to respond to immune challenge during overwintering

Authors

ŘEŘICHA, Michal, Pavel DOBEŠ (203 Czech Republic, belonging to the institution) and Michal KNAPP (guarantor)

Edition

Ecology and Evolution, Hoboken, Wiley, 2021, 2045-7758

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10613 Zoology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.167

RIV identification code

RIV/00216224:14310/21:00121284

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/ece3.7323

UT WoS

000627560300001

Keywords in English

antimicrobial response; bacterial challenge; Climate change; cold tolerance; fluctuating temperatures; haemocytes; innate immunity; invasive species; trade‐off

Abstract

V originále

Overwintering is a challenging period in the life of temperate insects. A limited energy budget characteristic of this period can result in reduced investment in immune system. Here, we investigated selected physiological and immunological parameters in laboratory‐reared and field‐collected harlequin ladybirds (Harmonia axyridis). For laboratory‐reared beetles, we focused on the effects of winter temperature regime (cold, average, or warm winter) on total haemocyte concentration aiming to investigate potential effects of ongoing climate change on immune system in overwintering insects. We recorded strong reduction in haemocyte concentration during winter; however, there were only limited effects of winter temperature regime on changes in haemocyte concentration in the course of overwintering. For field‐collected beetles, we measured additional parameters, specifically: total protein concentration, antimicrobial activity against Escherichia coli, and haemocyte concentration before and after overwintering. The field experiment did not investigate effects of winter temperature, but focused on changes in inducibility of insect immune system during overwintering, that is, measured parameters were compared between naïve beetles and those challenged by Escherichia coli. Haemocyte concentration decreased during overwintering, but only in individuals challenged by Escherichia coli. Prior to overwintering, the challenged beetles had a significantly higher haemocyte concentration compared to naïve beetles, whereas no difference was observed after overwintering. A similar pattern was observed also for antimicrobial activity against Escherichia coli as challenged beetles outperformed naïve beetles before overwintering, but not after winter. In both sexes, total protein concentration increased in the course of overwintering, but females had a significantly higher total protein concentration in their hemolymph compared to males. In general, our results revealed that insect’s ability to respond to an immune challenge is significantly reduced in the course of overwintering.

Citovat

ŘEŘICHA, Michal, Pavel DOBEŠ and Michal KNAPP. Changes in haemolymph parameters and insect ability to respond to immune challenge during overwintering. Ecology and Evolution. Hoboken: Wiley, 2021, vol. 11, No 9, p. 4267-4275. ISSN 2045-7758. Available from: https://dx.doi.org/10.1002/ece3.7323.

@article{1754476,
   author = {Řeřicha, Michal and Dobeš, Pavel and Knapp, Michal},
   article_location = {Hoboken},
   article_number = {9},
   doi = {http://dx.doi.org/10.1002/ece3.7323},
   keywords = {antimicrobial response; bacterial challenge; Climate change; cold tolerance; fluctuating temperatures; haemocytes; innate immunity; invasive species; trade‐off},
   language = {eng},
   issn = {2045-7758},
   journal = {Ecology and Evolution},
   title = {Changes in haemolymph parameters and insect ability to respond to immune challenge during overwintering},
   url = {https://doi.org/10.1002/ece3.7323},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1754476
AU  - Řeřicha, Michal - Dobeš, Pavel - Knapp, Michal
PY  - 2021
TI  - Changes in haemolymph parameters and insect ability to respond to immune challenge during overwintering
JF  - Ecology and Evolution
VL  - 11
IS  - 9
SP  - 4267-4275
EP  - 4267-4275
PB  - Wiley
SN  - 20457758
KW  - antimicrobial response
KW  - bacterial challenge
KW  - Climate change
KW  - cold tolerance
KW  - fluctuating temperatures
KW  - haemocytes
KW  - innate immunity
KW  - invasive species
KW  - trade‐off
UR  - https://doi.org/10.1002/ece3.7323
N2  - Overwintering is a challenging period in the life of temperate insects. A limited energy budget characteristic of this period can result in reduced investment in immune system. Here, we investigated selected physiological and immunological parameters in laboratory‐reared and field‐collected harlequin ladybirds (Harmonia axyridis). For laboratory‐reared beetles, we focused on the effects of winter temperature regime (cold, average, or warm winter) on total haemocyte concentration aiming to investigate potential effects of ongoing climate change on immune system in overwintering insects. We recorded strong reduction in haemocyte concentration during winter; however, there were only limited effects of winter temperature regime on changes in haemocyte concentration in the course of overwintering. For field‐collected beetles, we measured additional parameters, specifically: total protein concentration, antimicrobial activity against Escherichia coli, and haemocyte concentration before and after overwintering. The field experiment did not investigate effects of winter temperature, but focused on changes in inducibility of insect immune system during overwintering, that is, measured parameters were compared between naïve beetles and those challenged by Escherichia coli. Haemocyte concentration decreased during overwintering, but only in individuals challenged by Escherichia coli. Prior to overwintering, the challenged beetles had a significantly higher haemocyte concentration compared to naïve beetles, whereas no difference was observed after overwintering. A similar pattern was observed also for antimicrobial activity against Escherichia coli as challenged beetles outperformed naïve beetles before overwintering, but not after winter. In both sexes, total protein concentration increased in the course of overwintering, but females had a significantly higher total protein concentration in their hemolymph compared to males. In general, our results revealed that insect’s ability to respond to an immune challenge is significantly reduced in the course of overwintering.
ER  -

ŘEŘICHA, Michal, Pavel DOBEŠ and Michal KNAPP. Changes in haemolymph parameters and insect ability to respond to immune challenge during overwintering. \textit{Ecology and Evolution}. Hoboken: Wiley, 2021, vol.~11, No~9, p.~4267-4275. ISSN~2045-7758. Available from: https://dx.doi.org/10.1002/ece3.7323.

Detailed Information on Publication Record