| CHENG, Mingpan, Dehui QIU, Liezel TAMON, Eva IŠTVÁNKOVÁ, Pavlína VÍŠKOVÁ, Samir AMRANE, Aurore GUÉDIN, Jielin CHEN, Laurent LACROIX, Huangxian JU, Lukáš TRANTÍREK, Aleksandr B. SAHAKYAN, Jun ZHOU and Jean-Louis MERGNY. Thermal and pH stabilities of i‐DNA: confronting in vitro experiments with models and in‐cell NMR data. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. Wiley-VCH Verlag GmbH, 2021, vol. 60, No 18, p. 10286-10294. ISSN 1433-7851. Available from: https://dx.doi.org/10.1002/anie.202016801. |
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@article{1754819,
author = {Cheng, Mingpan and Qiu, Dehui and Tamon, Liezel and Ištvánková, Eva and Víšková, Pavlína and Amrane, Samir and Guédin, Aurore and Chen, Jielin and Lacroix, Laurent and Ju, Huangxian and Trantírek, Lukáš and Sahakyan, Aleksandr B. and Zhou, Jun and Mergny, JeanandLouis},
article_number = {18},
doi = {http://dx.doi.org/10.1002/anie.202016801},
keywords = {DNA; i-motif; thermal stability; pH transition; intracellular stability},
language = {eng},
issn = {1433-7851},
journal = {ANGEWANDTE CHEMIE-INTERNATIONAL EDITION},
title = {Thermal and pH stabilities of i‐DNA: confronting in vitro experiments with models and in‐cell NMR data},
url = {https://onlinelibrary.wiley.com/doi/10.1002/anie.202016801},
volume = {60},
year = {2021}
}
TY - JOUR
ID - 1754819
AU - Cheng, Mingpan - Qiu, Dehui - Tamon, Liezel - Ištvánková, Eva - Víšková, Pavlína - Amrane, Samir - Guédin, Aurore - Chen, Jielin - Lacroix, Laurent - Ju, Huangxian - Trantírek, Lukáš - Sahakyan, Aleksandr B. - Zhou, Jun - Mergny, Jean-Louis
PY - 2021
TI - Thermal and pH stabilities of i‐DNA: confronting in vitro experiments with models and in‐cell NMR data
JF - ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
VL - 60
IS - 18
SP - 10286-10294
EP - 10286-10294
PB - Wiley-VCH Verlag GmbH
SN - 14337851
KW - DNA
KW - i-motif
KW - thermal stability
KW - pH transition
KW - intracellular stability
UR - https://onlinelibrary.wiley.com/doi/10.1002/anie.202016801
N2 - Recent studies indicate that i‐DNA, a four‐stranded cytosine‐rich DNA also known as the i‐motif, is actually formed in vivo ; however, a systematic study on sequence effects on stability has been missing. Herein, an unprecedented number of different sequences (271) bearing four runs of 3‐6 cytosines with different spacer lengths has been tested. While i‐DNA stability is nearly independent on total spacer length, the central spacer plays a special role on stability. Stability also depends on the length of the C‐tracts at both acidic and neutral pHs. This study provides a global picture on i‐DNA stability thanks to the large size of the introduced data set; it reveals unexpected features and allows to conclude that determinants of i‐DNA stability do not mirror those of G‐quadruplexes. Our results illustrate the structural roles of loops and C‐tracts on i‐DNA stability, confirm its formation in cells, and allow establishing rules to predict its stability.
ER -
CHENG, Mingpan, Dehui QIU, Liezel TAMON, Eva IŠTVÁNKOVÁ, Pavlína VÍŠKOVÁ, Samir AMRANE, Aurore GUÉDIN, Jielin CHEN, Laurent LACROIX, Huangxian JU, Lukáš TRANTÍREK, Aleksandr B. SAHAKYAN, Jun ZHOU and Jean-Louis MERGNY. Thermal and pH stabilities of i‐DNA: confronting in vitro experiments with models and in‐cell NMR data. \textit{ANGEWANDTE CHEMIE-INTERNATIONAL EDITION}. Wiley-VCH Verlag GmbH, 2021, vol.~60, No~18, p.~10286-10294. ISSN~1433-7851. Available from: https://dx.doi.org/10.1002/anie.202016801.
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