Upconversion nanoparticles as labels for histopathological tissue evaluation

MICKERT, Matthias Jürgen, Zdeněk FARKA, Krzysztof KRAWCZYK, Antonín HLAVÁČEK, Julian BRANDMEIER, Stefan ANDERSSON-ENGELS, Anders SJÖGREN, Petr SKLÁDAL and Hans-Heiner GORRIS. Upconversion nanoparticles as labels for histopathological tissue evaluation. In UPCONline 2021. 2021.

Basic information

• Original name: Upconversion nanoparticles as labels for histopathological tissue evaluation
• Authors: MICKERT, Matthias Jürgen, Zdeněk FARKA, Krzysztof KRAWCZYK, Antonín HLAVÁČEK, Julian BRANDMEIER, Stefan ANDERSSON-ENGELS, Anders SJÖGREN, Petr SKLÁDAL and Hans-Heiner GORRIS.
• Edition: UPCONline 2021, 2021.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10406 Analytical chemistry
• Country of publisher: France
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Science
• Tags: International impact

Abstract

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are routine methods for the microscopic identification of cancerous cells in cell cultures and histological tissues. A growing population and longer life expectancy lead to an increase in tissue samples from cancer patients. Multiplexing and digital pathology can increase the sample throughput, improve the diagnosis, and reduce the analysis time. UCNPs are well suited for digital image analysis of biological tissues because the anti-Stokes emission under NIR excitation drastically reduces autofluorescence and light scattering, and their multiple narrow emission bands allow for multiplexed applications. In this work, we designed streptavidin-modified UCNPs (SA-UCNP) for the detection of the breast cancer biomarker HER2 on the surface of model breast cancer cell lines and breast cancer tissue. The SA-UCNP conjugate achieved a significantly higher signal-to-background ratio compared to a conventional labelling using a streptavidin-carboxyfluorescein conjugate under the same experimental conditions. An in-house made UCNP-conjugate from Lumito was used to label HER2 in breast cancer tissue. The imaging was performed by Lumito’s prototype scanner capable of combining UCNP upconversion imaging and haematoxylin brightfield imaging at a 20× magnification.

Links

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

• LTAB19011, research and development project: Name: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel

Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION