Upconversion nanoparticles as labels for histopathological
tissue evaluation

a 2021

Upconversion nanoparticles as labels for histopathological tissue evaluation

MICKERT, Matthias Jürgen, Zdeněk FARKA, Krzysztof KRAWCZYK, Antonín HLAVÁČEK, Julian BRANDMEIER et. al.

Basic information

Original name

Upconversion nanoparticles as labels for histopathological tissue evaluation

Authors

MICKERT, Matthias Jürgen, Zdeněk FARKA, Krzysztof KRAWCZYK, Antonín HLAVÁČEK, Julian BRANDMEIER, Stefan ANDERSSON-ENGELS, Anders SJÖGREN, Petr SKLÁDAL and Hans-Heiner GORRIS

Edition

UPCONline 2021, 2021

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10406 Analytical chemistry

Country of publisher

France

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

Abstract

V originále

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are routine methods for the microscopic identification of cancerous cells in cell cultures and histological tissues. A growing population and longer life expectancy lead to an increase in tissue samples from cancer patients. Multiplexing and digital pathology can increase the sample throughput, improve the diagnosis, and reduce the analysis time. UCNPs are well suited for digital image analysis of biological tissues because the anti-Stokes emission under NIR excitation drastically reduces autofluorescence and light scattering, and their multiple narrow emission bands allow for multiplexed applications. In this work, we designed streptavidin-modified UCNPs (SA-UCNP) for the detection of the breast cancer biomarker HER2 on the surface of model breast cancer cell lines and breast cancer tissue. The SA-UCNP conjugate achieved a significantly higher signal-to-background ratio compared to a conventional labelling using a streptavidin-carboxyfluorescein conjugate under the same experimental conditions. An in-house made UCNP-conjugate from Lumito was used to label HER2 in breast cancer tissue. The imaging was performed by Lumito’s prototype scanner capable of combining UCNP upconversion imaging and haematoxylin brightfield imaging at a 20× magnification.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

LTAB19011, research and development project

Name: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel

Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION

Citovat

MICKERT, Matthias Jürgen, Zdeněk FARKA, Krzysztof KRAWCZYK, Antonín HLAVÁČEK, Julian BRANDMEIER, Stefan ANDERSSON-ENGELS, Anders SJÖGREN, Petr SKLÁDAL and Hans-Heiner GORRIS. Upconversion nanoparticles as labels for histopathological tissue evaluation. In UPCONline 2021. 2021.

@proceedings{1757237,
   author = {Mickert, Matthias Jürgen and Farka, Zdeněk and Krawczyk, Krzysztof and Hlaváček, Antonín and Brandmeier, Julian and AnderssonandEngels, Stefan and Sjögren, Anders and Skládal, Petr and Gorris, HansandHeiner},
   booktitle = {UPCONline 2021},
   language = {eng},
   title = {Upconversion nanoparticles as labels for histopathological tissue evaluation},
   year = {2021}
}

TY  - CONF
ID  - 1757237
AU  - Mickert, Matthias Jürgen - Farka, Zdeněk - Krawczyk, Krzysztof - Hlaváček, Antonín - Brandmeier, Julian - Andersson-Engels, Stefan - Sjögren, Anders - Skládal, Petr - Gorris, Hans-Heiner
PY  - 2021
TI  - Upconversion nanoparticles as labels for histopathological tissue evaluation
N2  - Immunocytochemistry (ICC) and immunohistochemistry (IHC) are routine methods for the microscopic identification of cancerous cells in cell cultures and histological tissues. A growing population and longer life expectancy lead to an increase in tissue samples from cancer patients. Multiplexing and digital pathology can increase the sample throughput, improve the diagnosis, and reduce the analysis time. UCNPs are well suited for digital image analysis of biological tissues because the anti-Stokes emission under NIR excitation drastically reduces autofluorescence and light scattering, and their multiple narrow emission bands allow for multiplexed applications. In this work, we designed streptavidin-modified UCNPs (SA-UCNP) for the detection of the breast cancer biomarker HER2 on the surface of model breast cancer cell lines and breast cancer tissue. The SA-UCNP conjugate achieved a significantly higher signal-to-background ratio compared to a conventional labelling using a streptavidin-carboxyfluorescein conjugate under the same experimental conditions. An in-house made UCNP-conjugate from Lumito was used to label HER2 in breast cancer tissue. The imaging was performed by Lumito’s prototype scanner capable of combining UCNP upconversion imaging and haematoxylin brightfield imaging at a 20× magnification.
ER  -

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