| BERNATOVA, S., Katarína REBROŠOVÁ, Z. PILAT, M. SERY, A. GJEVIK, O. SAMEK, J. JEZEK, M. SILER, M. KIZOVSKY, T. KLEMENTOVA, Veronika HOLÁ, Filip RŮŽIČKA and P. ZEMANEK. Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers. European Physical Journal Plus. HEIDELBERG: SPRINGER HEIDELBERG, 2021, vol. 136, No 2, p. 1-10. ISSN 2190-5444. Available from: https://dx.doi.org/10.1140/epjp/s13360-021-01152-1. |
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@article{1758059,
author = {Bernatova, S. and Rebrošová, Katarína and Pilat, Z. and Sery, M. and Gjevik, A. and Samek, O. and Jezek, J. and Siler, M. and Kizovsky, M. and Klementova, T. and Holá, Veronika and Růžička, Filip and Zemanek, P.},
article_location = {HEIDELBERG},
article_number = {2},
doi = {http://dx.doi.org/10.1140/epjp/s13360-021-01152-1},
keywords = {antibiotic sensitivity; Staphylococcus aureus; Raman tweezers},
language = {eng},
issn = {2190-5444},
journal = {European Physical Journal Plus},
title = {Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers},
url = {https://link.springer.com/article/10.1140%2Fepjp%2Fs13360-021-01152-1},
volume = {136},
year = {2021}
}
TY - JOUR
ID - 1758059
AU - Bernatova, S. - Rebrošová, Katarína - Pilat, Z. - Sery, M. - Gjevik, A. - Samek, O. - Jezek, J. - Siler, M. - Kizovsky, M. - Klementova, T. - Holá, Veronika - Růžička, Filip - Zemanek, P.
PY - 2021
TI - Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers
JF - European Physical Journal Plus
VL - 136
IS - 2
SP - 1-10
EP - 1-10
PB - SPRINGER HEIDELBERG
SN - 21905444
KW - antibiotic sensitivity
KW - Staphylococcus aureus
KW - Raman tweezers
UR - https://link.springer.com/article/10.1140%2Fepjp%2Fs13360-021-01152-1
N2 - Methicillin-resistant Staphylococcus aureus is a bacterium pathogenic to humans and a leading cause of the hospital-acquired infections, causing significant increase in morbidity and mortality. Conventional antibiotic sensitivity testing requires culturing of the isolated pathogen in the presence of antibiotics, and it takes at least 48 hours. Comparatively faster determination of bacterial sensitivity to antibiotics can be achieved with Raman tweezers-an analytical method based on Raman spectroscopy and optical trapping. This article demonstrates the effectiveness of this approach for the discrimination between a methicillin-resistant and a methicillin-sensitive strain of Staphylococcus aureus in about 4 hours from a microliter volume of the bacterial sample. We found that the antibiotic-induced changes in the bacterial cells influenced the ratio of the Raman signals of nucleic acids to phenylalanine. This points to the antibiotic causing cell lysis and the associated loss of nucleic acids from the cytoplasm.
ER -
BERNATOVA, S., Katarína REBROŠOVÁ, Z. PILAT, M. SERY, A. GJEVIK, O. SAMEK, J. JEZEK, M. SILER, M. KIZOVSKY, T. KLEMENTOVA, Veronika HOLÁ, Filip RŮŽIČKA and P. ZEMANEK. Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers. \textit{European Physical Journal Plus}. HEIDELBERG: SPRINGER HEIDELBERG, 2021, vol.~136, No~2, p.~1-10. ISSN~2190-5444. Available from: https://dx.doi.org/10.1140/epjp/s13360-021-01152-1.
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