Rapid detection of antibiotic sensitivity of Staphylococcus
aureus by Raman tweezers

J 2021

Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers

BERNATOVA, S., Katarína REBROŠOVÁ, Z. PILAT, M. SERY, A. GJEVIK et. al.

Basic information

Original name

Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers

Authors

BERNATOVA, S. (203 Czech Republic, guarantor), Katarína REBROŠOVÁ (703 Slovakia, belonging to the institution), Z. PILAT (203 Czech Republic), M. SERY (203 Czech Republic), A. GJEVIK (203 Czech Republic), O. SAMEK (203 Czech Republic), J. JEZEK (203 Czech Republic), M. SILER (203 Czech Republic), M. KIZOVSKY (203 Czech Republic), T. KLEMENTOVA (203 Czech Republic), Veronika HOLÁ (203 Czech Republic, belonging to the institution), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution) and P. ZEMANEK (203 Czech Republic)

Edition

European Physical Journal Plus, HEIDELBERG, SPRINGER HEIDELBERG, 2021, 2190-5444

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10301 Atomic, molecular and chemical physics

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.758

RIV identification code

RIV/00216224:14110/21:00121360

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1140/epjp/s13360-021-01152-1

UT WoS

000621061800003

Keywords in English

antibiotic sensitivity; Staphylococcus aureus; Raman tweezers

Abstract

V originále

Methicillin-resistant Staphylococcus aureus is a bacterium pathogenic to humans and a leading cause of the hospital-acquired infections, causing significant increase in morbidity and mortality. Conventional antibiotic sensitivity testing requires culturing of the isolated pathogen in the presence of antibiotics, and it takes at least 48 hours. Comparatively faster determination of bacterial sensitivity to antibiotics can be achieved with Raman tweezers-an analytical method based on Raman spectroscopy and optical trapping. This article demonstrates the effectiveness of this approach for the discrimination between a methicillin-resistant and a methicillin-sensitive strain of Staphylococcus aureus in about 4 hours from a microliter volume of the bacterial sample. We found that the antibiotic-induced changes in the bacterial cells influenced the ratio of the Raman signals of nucleic acids to phenylalanine. This points to the antibiotic causing cell lysis and the associated loss of nucleic acids from the cytoplasm.

Citovat

BERNATOVA, S., Katarína REBROŠOVÁ, Z. PILAT, M. SERY, A. GJEVIK, O. SAMEK, J. JEZEK, M. SILER, M. KIZOVSKY, T. KLEMENTOVA, Veronika HOLÁ, Filip RŮŽIČKA and P. ZEMANEK. Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers. European Physical Journal Plus. HEIDELBERG: SPRINGER HEIDELBERG, 2021, vol. 136, No 2, p. 1-10. ISSN 2190-5444. Available from: https://dx.doi.org/10.1140/epjp/s13360-021-01152-1.

@article{1758059,
   author = {Bernatova, S. and Rebrošová, Katarína and Pilat, Z. and Sery, M. and Gjevik, A. and Samek, O. and Jezek, J. and Siler, M. and Kizovsky, M. and Klementova, T. and Holá, Veronika and Růžička, Filip and Zemanek, P.},
   article_location = {HEIDELBERG},
   article_number = {2},
   doi = {http://dx.doi.org/10.1140/epjp/s13360-021-01152-1},
   keywords = {antibiotic sensitivity; Staphylococcus aureus; Raman tweezers},
   language = {eng},
   issn = {2190-5444},
   journal = {European Physical Journal Plus},
   title = {Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers},
   url = {https://link.springer.com/article/10.1140%2Fepjp%2Fs13360-021-01152-1},
   volume = {136},
   year = {2021}
}

TY  - JOUR
ID  - 1758059
AU  - Bernatova, S. - Rebrošová, Katarína - Pilat, Z. - Sery, M. - Gjevik, A. - Samek, O. - Jezek, J. - Siler, M. - Kizovsky, M. - Klementova, T. - Holá, Veronika - Růžička, Filip - Zemanek, P.
PY  - 2021
TI  - Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers
JF  - European Physical Journal Plus
VL  - 136
IS  - 2
SP  - 1-10
EP  - 1-10
PB  - SPRINGER HEIDELBERG
SN  - 21905444
KW  - antibiotic sensitivity
KW  - Staphylococcus aureus
KW  - Raman tweezers
UR  - https://link.springer.com/article/10.1140%2Fepjp%2Fs13360-021-01152-1
N2  - Methicillin-resistant Staphylococcus aureus is a bacterium pathogenic to humans and a leading cause of the hospital-acquired infections, causing significant increase in morbidity and mortality. Conventional antibiotic sensitivity testing requires culturing of the isolated pathogen in the presence of antibiotics, and it takes at least 48 hours. Comparatively faster determination of bacterial sensitivity to antibiotics can be achieved with Raman tweezers-an analytical method based on Raman spectroscopy and optical trapping. This article demonstrates the effectiveness of this approach for the discrimination between a methicillin-resistant and a methicillin-sensitive strain of Staphylococcus aureus in about 4 hours from a microliter volume of the bacterial sample. We found that the antibiotic-induced changes in the bacterial cells influenced the ratio of the Raman signals of nucleic acids to phenylalanine. This points to the antibiotic causing cell lysis and the associated loss of nucleic acids from the cytoplasm.
ER  -

BERNATOVA, S., Katarína REBROŠOVÁ, Z. PILAT, M. SERY, A. GJEVIK, O. SAMEK, J. JEZEK, M. SILER, M. KIZOVSKY, T. KLEMENTOVA, Veronika HOLÁ, Filip RŮŽIČKA and P. ZEMANEK. Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers. \textit{European Physical Journal Plus}. HEIDELBERG: SPRINGER HEIDELBERG, 2021, vol.~136, No~2, p.~1-10. ISSN~2190-5444. Available from: https://dx.doi.org/10.1140/epjp/s13360-021-01152-1.

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