Actinotignum schaalii: Relation to Concomitants and Connection
to Patients’ Conditions in Polymicrobial Biofilms of Urinary
Tract Catheters and Urines

J 2021

Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines

KOTÁSKOVÁ, Iva, Vít SYROVÁTKA, Hana OBRUČOVÁ, Petra VÍDEŇSKÁ, Barbora ZWINSOVÁ et. al.

Basic information

Original name

Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines

Authors

KOTÁSKOVÁ, Iva (203 Czech Republic, belonging to the institution), Vít SYROVÁTKA (203 Czech Republic, belonging to the institution), Hana OBRUČOVÁ (203 Czech Republic), Petra VÍDEŇSKÁ (203 Czech Republic, belonging to the institution), Barbora ZWINSOVÁ (203 Czech Republic, belonging to the institution), Veronika HOLÁ (203 Czech Republic, belonging to the institution), Eva BLAŠTÍKOVÁ (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution) and Tomáš FREIBERGER (203 Czech Republic, guarantor, belonging to the institution)

Edition

Microorganisms, Basel, MDPI, 2021, 2076-2607

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.926

RIV identification code

RIV/00216224:14110/21:00120090

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/microorganisms9030669

UT WoS

000635249300001

Keywords in English

Actinotignum; Actinobaculum; hydronephrosis; urinary catheter; ureteral stent; Double-J catheter; Propionimicrobium; Fusobacterium; urobiome; microbiome; colonisation

Abstract

V originále

Actinotignum schaalii is an emerging, opportunistic pathogen and its connection to non-infectious diseases and conditions, such as prostate or bladder cancer, or chronic inflammation has been proposed. Here, we analyzed 297 urine, ureteral and urinary catheter samples from 128 patients by Polymerase Chain Reaction followed by Denaturing Gradient Gel Electrophoresis and Sequencing (PCR-DGGE-S), and culture, and 29 of these samples also by 16S rRNA Illumina sequencing, to establish A. schaalii’s prevalence in urinary tract-related samples, its relation to other bacteria, and its potential association with patients’ conditions and samples’ characteristics. A. schaalii-positive samples were significantly more diverse than A. schaalii negative and between-group diversity was higher than intra-group. Propionimicrobium lymphophilum, Fusobacterium nucleatum, Veillonella sp., Morganella sp., and Aerococcus sp. were significantly more often present in A. schaalii-positive samples; thus, we suggest these species are A. schaalii’s concomitants, while Enterobacter and Staphylococcaceae were more often identified in A. schaalii-negative samples; therefore, we propose A. schaalii and these species are mutually exclusive. Additionally, a significantly higher A. schaalii prevalence in patients with ureter stricture associated hydronephrosis (p = 0.020) was noted. We suggest that A. schaalii could be an early polybacterial biofilm colonizer, together with concomitant species, known for pro-inflammatory features.

Links

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

LM2015085, research and development project

Name: CERIT Scientific Cloud (Acronym: CERIT-SC)

Investor: Ministry of Education, Youth and Sports of the CR, CERIT Scientific Cloud

MUNI/A/1099/2019, interní kód MU

Name: Faktory nespecifické imunity u některých imunopatologických stavů (Acronym: nespecif. imunita)

Investor: Masaryk University, Category A

MUNI/A/1412/2020, interní kód MU

Name: Patogenetický význam nespecifické imunity u některých poruch imunitního systému (Acronym: Nespecifická imunita)

Investor: Masaryk University

NV16-31593A, research and development project

Name: Polymikrobiální biofilm jako komplexní mikrobiom v rámci léčby nozokomiálních infekcí

Citovat

KOTÁSKOVÁ, Iva, Vít SYROVÁTKA, Hana OBRUČOVÁ, Petra VÍDEŇSKÁ, Barbora ZWINSOVÁ, Veronika HOLÁ, Eva BLAŠTÍKOVÁ, Filip RŮŽIČKA and Tomáš FREIBERGER. Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines. Microorganisms. Basel: MDPI, 2021, vol. 9, No 3, p. 1-23. ISSN 2076-2607. Available from: https://dx.doi.org/10.3390/microorganisms9030669.

@article{1758116,
   author = {Kotásková, Iva and Syrovátka, Vít and Obručová, Hana and Vídeňská, Petra and Zwinsová, Barbora and Holá, Veronika and Blaštíková, Eva and Růžička, Filip and Freiberger, Tomáš},
   article_location = {Basel},
   article_number = {3},
   doi = {http://dx.doi.org/10.3390/microorganisms9030669},
   keywords = {Actinotignum; Actinobaculum; hydronephrosis; urinary catheter; ureteral stent; Double-J catheter; Propionimicrobium; Fusobacterium; urobiome; microbiome; colonisation},
   language = {eng},
   issn = {2076-2607},
   journal = {Microorganisms},
   title = {Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines},
   url = {https://www.mdpi.com/2076-2607/9/3/669},
   volume = {9},
   year = {2021}
}

TY  - JOUR
ID  - 1758116
AU  - Kotásková, Iva - Syrovátka, Vít - Obručová, Hana - Vídeňská, Petra - Zwinsová, Barbora - Holá, Veronika - Blaštíková, Eva - Růžička, Filip - Freiberger, Tomáš
PY  - 2021
TI  - Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines
JF  - Microorganisms
VL  - 9
IS  - 3
SP  - 1-23
EP  - 1-23
PB  - MDPI
SN  - 20762607
KW  - Actinotignum
KW  - Actinobaculum
KW  - hydronephrosis
KW  - urinary catheter
KW  - ureteral stent
KW  - Double-J catheter
KW  - Propionimicrobium
KW  - Fusobacterium
KW  - urobiome
KW  - microbiome
KW  - colonisation
UR  - https://www.mdpi.com/2076-2607/9/3/669
N2  - Actinotignum schaalii is an emerging, opportunistic pathogen and its connection to non-infectious diseases and conditions, such as prostate or bladder cancer, or chronic inflammation has been proposed. Here, we analyzed 297 urine, ureteral and urinary catheter samples from 128 patients by Polymerase Chain Reaction followed by Denaturing Gradient Gel Electrophoresis and Sequencing (PCR-DGGE-S), and culture, and 29 of these samples also by 16S rRNA Illumina sequencing, to establish A. schaalii’s prevalence in urinary tract-related samples, its relation to other bacteria, and its potential association with patients’ conditions and samples’ characteristics. A. schaalii-positive samples were significantly more diverse than A. schaalii negative and between-group diversity was higher than intra-group. Propionimicrobium lymphophilum, Fusobacterium nucleatum, Veillonella sp., Morganella sp., and Aerococcus sp. were significantly more often present in A. schaalii-positive samples; thus, we suggest these species are A. schaalii’s concomitants, while Enterobacter and Staphylococcaceae were more often identified in A. schaalii-negative samples; therefore, we propose A. schaalii and these species are mutually exclusive. Additionally, a significantly higher A. schaalii prevalence in patients with ureter stricture associated hydronephrosis (p = 0.020) was noted. We suggest that A. schaalii could be an early polybacterial biofilm colonizer, together with concomitant species, known for pro-inflammatory features.
ER  -

KOTÁSKOVÁ, Iva, Vít SYROVÁTKA, Hana OBRUČOVÁ, Petra VÍDEŇSKÁ, Barbora ZWINSOVÁ, Veronika HOLÁ, Eva BLAŠTÍKOVÁ, Filip RŮŽIČKA and Tomáš FREIBERGER. Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines. \textit{Microorganisms}. Basel: MDPI, 2021, vol.~9, No~3, p.~1-23. ISSN~2076-2607. Available from: https://dx.doi.org/10.3390/microorganisms9030669.

Detailed Information on Publication Record