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@article{1758116,
author = {Kotásková, Iva and Syrovátka, Vít and Obručová, Hana and Vídeňská, Petra and Zwinsová, Barbora and Holá, Veronika and Blaštíková, Eva and Růžička, Filip and Freiberger, Tomáš},
article_location = {Basel},
article_number = {3},
doi = {http://dx.doi.org/10.3390/microorganisms9030669},
keywords = {Actinotignum; Actinobaculum; hydronephrosis; urinary catheter; ureteral stent; Double-J catheter; Propionimicrobium; Fusobacterium; urobiome; microbiome; colonisation},
language = {eng},
issn = {2076-2607},
journal = {Microorganisms},
title = {Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines},
url = {https://www.mdpi.com/2076-2607/9/3/669},
volume = {9},
year = {2021}
}
TY - JOUR
ID - 1758116
AU - Kotásková, Iva - Syrovátka, Vít - Obručová, Hana - Vídeňská, Petra - Zwinsová, Barbora - Holá, Veronika - Blaštíková, Eva - Růžička, Filip - Freiberger, Tomáš
PY - 2021
TI - Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines
JF - Microorganisms
VL - 9
IS - 3
SP - 1-23
EP - 1-23
PB - MDPI
SN - 20762607
KW - Actinotignum
KW - Actinobaculum
KW - hydronephrosis
KW - urinary catheter
KW - ureteral stent
KW - Double-J catheter
KW - Propionimicrobium
KW - Fusobacterium
KW - urobiome
KW - microbiome
KW - colonisation
UR - https://www.mdpi.com/2076-2607/9/3/669
N2 - Actinotignum schaalii is an emerging, opportunistic pathogen and its connection to non-infectious diseases and conditions, such as prostate or bladder cancer, or chronic inflammation has been proposed. Here, we analyzed 297 urine, ureteral and urinary catheter samples from 128 patients by Polymerase Chain Reaction followed by Denaturing Gradient Gel Electrophoresis and Sequencing (PCR-DGGE-S), and culture, and 29 of these samples also by 16S rRNA Illumina sequencing, to establish A. schaalii’s prevalence in urinary tract-related samples, its relation to other bacteria, and its potential association with patients’ conditions and samples’ characteristics. A. schaalii-positive samples were significantly more diverse than A. schaalii negative and between-group diversity was higher than intra-group. Propionimicrobium lymphophilum, Fusobacterium nucleatum, Veillonella sp., Morganella sp., and Aerococcus sp. were significantly more often present in A. schaalii-positive samples; thus, we suggest these species are A. schaalii’s concomitants, while Enterobacter and Staphylococcaceae were more often identified in A. schaalii-negative samples; therefore, we propose A. schaalii and these species are mutually exclusive. Additionally, a significantly higher A. schaalii prevalence in patients with ureter stricture associated hydronephrosis (p = 0.020) was noted. We suggest that A. schaalii could be an early polybacterial biofilm colonizer, together with concomitant species, known for pro-inflammatory features.
ER -
KOTÁSKOVÁ, Iva, Vít SYROVÁTKA, Hana OBRUČOVÁ, Petra VÍDEŇSKÁ, Barbora ZWINSOVÁ, Veronika HOLÁ, Eva BLAŠTÍKOVÁ, Filip RŮŽIČKA and Tomáš FREIBERGER. Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines. \textit{Microorganisms}. Basel: MDPI, 2021, vol.~9, No~3, p.~1-23. ISSN~2076-2607. Available from: https://dx.doi.org/10.3390/microorganisms9030669.
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