Laser-induced breakdown spectroscopy as a readout method for
immunocytochemistry with upconversion nanoparticles

J 2021

Laser-induced breakdown spectroscopy as a readout method for immunocytochemistry with upconversion nanoparticles

POŘÍZKA, Pavel, Karolína VYTISKOVÁ, Radka OBOŘILOVÁ, Matěj PASTUCHA, Ivan GÁBRIŠ et. al.

Basic information

Original name

Laser-induced breakdown spectroscopy as a readout method for immunocytochemistry with upconversion nanoparticles

Authors

POŘÍZKA, Pavel, Karolína VYTISKOVÁ (203 Czech Republic, belonging to the institution), Radka OBOŘILOVÁ (203 Czech Republic, belonging to the institution), Matěj PASTUCHA (203 Czech Republic, belonging to the institution), Ivan GÁBRIŠ (703 Slovakia, belonging to the institution), Julian C. BRANDMEIER, Pavlína MODLITBOVÁ (203 Czech Republic), Hans-Heiner GORRIS (276 Germany), Karel NOVOTNÝ (203 Czech Republic, guarantor, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution), Jozef KAISER (203 Czech Republic) and Zdeněk FARKA (203 Czech Republic, belonging to the institution)

Edition

Microchimica Acta, Springer-Verlag GmbH Austria, 2021, 0026-3672

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Austria

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.408

RIV identification code

RIV/00216224:14310/21:00118920

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s00604-021-04816-y

UT WoS

000636742200003

Keywords in English

Immunocytochemistry; Immunohistochemistry; Laser-induced breakdown spectroscopy; Tag-LIBS; Photon-upconversion nanoparticles

Abstract

V originále

Immunohistochemistry (IHC) and immunocytochemistry (ICC) are widely used to identify cancerous cells within tissues and cell cultures. Even though the optical microscopy evaluation is considered the gold standard, the limited range of useful labels and narrow multiplexing capabilities create an imminent need for alternative readout techniques. Laser-induced breakdown spectroscopy (LIBS) enables large-scale multi-elemental analysis of the surface of biological samples, e.g., thin section or cell pellet. It is, therefore, a potential alternative for IHC and ICC readout of various labels or tags (Tag-LIBS approach). Here, we introduce Tag-LIBS as a method for the specific determination of HER2 biomarker. The cell pellets were labeled with streptavidin-conjugated upconversion nanoparticles (UCNP) through a primary anti-HER2 antibody and a biotinylated secondary antibody. The LIBS scanning enabled detecting the characteristic elemental signature of yttrium as a principal constituent of UCNP, thus indirectly providing a reliable way to differentiate between HER2-positive BT-474 cells and HER2-negative MDA-MB-231 cells. The comparison of results with upconversion optical microscopy and luminescence intensity scanning confirmed that LIBS is a promising alternative for the IHC and ICC readout.

Links

GJ20-30004Y, research and development project

Name: Nové strukturní motivy pro rozšíření možností lineárních polymethinových barviv

Investor: Czech Science Foundation

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

LTAB19011, research and development project

Name: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel

Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION

MUNI/A/1390/2020, interní kód MU

Name: Analytická a fyzikální chemie ve výzkumu biologických, geologických a syntetických materiálů (Acronym: BIOGEOSYNT)

Investor: Masaryk University

MUNI/A/1604/2020, interní kód MU

Name: Podpora biochemického výzkumu v roce 2021

Investor: Masaryk University

90110, large research infrastructures

Name: CzechNanoLab

Citovat

POŘÍZKA, Pavel, Karolína VYTISKOVÁ, Radka OBOŘILOVÁ, Matěj PASTUCHA, Ivan GÁBRIŠ, Julian C. BRANDMEIER, Pavlína MODLITBOVÁ, Hans-Heiner GORRIS, Karel NOVOTNÝ, Petr SKLÁDAL, Jozef KAISER and Zdeněk FARKA. Laser-induced breakdown spectroscopy as a readout method for immunocytochemistry with upconversion nanoparticles. Microchimica Acta. Springer-Verlag GmbH Austria, 2021, vol. 188, No 5, p. "147", 10 pp. ISSN 0026-3672. Available from: https://dx.doi.org/10.1007/s00604-021-04816-y.

@article{1758139,
   author = {Pořízka, Pavel and Vytisková, Karolína and Obořilová, Radka and Pastucha, Matěj and Gábriš, Ivan and Brandmeier, Julian C. and Modlitbová, Pavlína and Gorris, HansandHeiner and Novotný, Karel and Skládal, Petr and Kaiser, Jozef and Farka, Zdeněk},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s00604-021-04816-y},
   keywords = {Immunocytochemistry; Immunohistochemistry; Laser-induced breakdown spectroscopy; Tag-LIBS; Photon-upconversion nanoparticles},
   language = {eng},
   issn = {0026-3672},
   journal = {Microchimica Acta},
   title = {Laser-induced breakdown spectroscopy as a readout method for immunocytochemistry with upconversion nanoparticles},
   url = {https://link.springer.com/article/10.1007/s00604-021-04816-y},
   volume = {188},
   year = {2021}
}

TY  - JOUR
ID  - 1758139
AU  - Pořízka, Pavel - Vytisková, Karolína - Obořilová, Radka - Pastucha, Matěj - Gábriš, Ivan - Brandmeier, Julian C. - Modlitbová, Pavlína - Gorris, Hans-Heiner - Novotný, Karel - Skládal, Petr - Kaiser, Jozef - Farka, Zdeněk
PY  - 2021
TI  - Laser-induced breakdown spectroscopy as a readout method for immunocytochemistry with upconversion nanoparticles
JF  - Microchimica Acta
VL  - 188
IS  - 5
SP  - "147"
EP  - "147"
PB  - Springer-Verlag GmbH Austria
SN  - 00263672
KW  - Immunocytochemistry
KW  - Immunohistochemistry
KW  - Laser-induced breakdown spectroscopy
KW  - Tag-LIBS
KW  - Photon-upconversion nanoparticles
UR  - https://link.springer.com/article/10.1007/s00604-021-04816-y
N2  - Immunohistochemistry (IHC) and immunocytochemistry (ICC) are widely used to identify cancerous cells within tissues and cell cultures. Even though the optical microscopy evaluation is considered the gold standard, the limited range of useful labels and narrow multiplexing capabilities create an imminent need for alternative readout techniques. Laser-induced breakdown spectroscopy (LIBS) enables large-scale multi-elemental analysis of the surface of biological samples, e.g., thin section or cell pellet. It is, therefore, a potential alternative for IHC and ICC readout of various labels or tags (Tag-LIBS approach). Here, we introduce Tag-LIBS as a method for the specific determination of HER2 biomarker. The cell pellets were labeled with streptavidin-conjugated upconversion nanoparticles (UCNP) through a primary anti-HER2 antibody and a biotinylated secondary antibody. The LIBS scanning enabled detecting the characteristic elemental signature of yttrium as a principal constituent of UCNP, thus indirectly providing a reliable way to differentiate between HER2-positive BT-474 cells and HER2-negative MDA-MB-231 cells. The comparison of results with upconversion optical microscopy and luminescence intensity scanning confirmed that LIBS is a promising alternative for the IHC and ICC readout.
ER  -

POŘÍZKA, Pavel, Karolína VYTISKOVÁ, Radka OBOŘILOVÁ, Matěj PASTUCHA, Ivan GÁBRIŠ, Julian C. BRANDMEIER, Pavlína MODLITBOVÁ, Hans-Heiner GORRIS, Karel NOVOTNÝ, Petr SKLÁDAL, Jozef KAISER and Zdeněk FARKA. Laser-induced breakdown spectroscopy as a readout method for immunocytochemistry with upconversion nanoparticles. \textit{Microchimica Acta}. Springer-Verlag GmbH Austria, 2021, vol.~188, No~5, p.~''147'', 10 pp. ISSN~0026-3672. Available from: https://dx.doi.org/10.1007/s00604-021-04816-y.

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