| POŘÍZKA, Pavel, Karolína VYTISKOVÁ, Radka OBOŘILOVÁ, Matěj PASTUCHA, Ivan GÁBRIŠ, Julian C. BRANDMEIER, Pavlína MODLITBOVÁ, Hans-Heiner GORRIS, Karel NOVOTNÝ, Petr SKLÁDAL, Jozef KAISER and Zdeněk FARKA. Laser-induced breakdown spectroscopy as a readout method for immunocytochemistry with upconversion nanoparticles. Microchimica Acta. Springer-Verlag GmbH Austria, 2021, vol. 188, No 5, p. "147", 10 pp. ISSN 0026-3672. Available from: https://dx.doi.org/10.1007/s00604-021-04816-y. |
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@article{1758139,
author = {Pořízka, Pavel and Vytisková, Karolína and Obořilová, Radka and Pastucha, Matěj and Gábriš, Ivan and Brandmeier, Julian C. and Modlitbová, Pavlína and Gorris, HansandHeiner and Novotný, Karel and Skládal, Petr and Kaiser, Jozef and Farka, Zdeněk},
article_number = {5},
doi = {http://dx.doi.org/10.1007/s00604-021-04816-y},
keywords = {Immunocytochemistry; Immunohistochemistry; Laser-induced breakdown spectroscopy; Tag-LIBS; Photon-upconversion nanoparticles},
language = {eng},
issn = {0026-3672},
journal = {Microchimica Acta},
title = {Laser-induced breakdown spectroscopy as a readout method for immunocytochemistry with upconversion nanoparticles},
url = {https://link.springer.com/article/10.1007/s00604-021-04816-y},
volume = {188},
year = {2021}
}
TY - JOUR
ID - 1758139
AU - Pořízka, Pavel - Vytisková, Karolína - Obořilová, Radka - Pastucha, Matěj - Gábriš, Ivan - Brandmeier, Julian C. - Modlitbová, Pavlína - Gorris, Hans-Heiner - Novotný, Karel - Skládal, Petr - Kaiser, Jozef - Farka, Zdeněk
PY - 2021
TI - Laser-induced breakdown spectroscopy as a readout method for immunocytochemistry with upconversion nanoparticles
JF - Microchimica Acta
VL - 188
IS - 5
SP - "147"
EP - "147"
PB - Springer-Verlag GmbH Austria
SN - 00263672
KW - Immunocytochemistry
KW - Immunohistochemistry
KW - Laser-induced breakdown spectroscopy
KW - Tag-LIBS
KW - Photon-upconversion nanoparticles
UR - https://link.springer.com/article/10.1007/s00604-021-04816-y
N2 - Immunohistochemistry (IHC) and immunocytochemistry (ICC) are widely used to identify cancerous cells within tissues and cell cultures. Even though the optical microscopy evaluation is considered the gold standard, the limited range of useful labels and narrow multiplexing capabilities create an imminent need for alternative readout techniques. Laser-induced breakdown spectroscopy (LIBS) enables large-scale multi-elemental analysis of the surface of biological samples, e.g., thin section or cell pellet. It is, therefore, a potential alternative for IHC and ICC readout of various labels or tags (Tag-LIBS approach). Here, we introduce Tag-LIBS as a method for the specific determination of HER2 biomarker. The cell pellets were labeled with streptavidin-conjugated upconversion nanoparticles (UCNP) through a primary anti-HER2 antibody and a biotinylated secondary antibody. The LIBS scanning enabled detecting the characteristic elemental signature of yttrium as a principal constituent of UCNP, thus indirectly providing a reliable way to differentiate between HER2-positive BT-474 cells and HER2-negative MDA-MB-231 cells. The comparison of results with upconversion optical microscopy and luminescence intensity scanning confirmed that LIBS is a promising alternative for the IHC and ICC readout.
ER -
POŘÍZKA, Pavel, Karolína VYTISKOVÁ, Radka OBOŘILOVÁ, Matěj PASTUCHA, Ivan GÁBRIŠ, Julian C. BRANDMEIER, Pavlína MODLITBOVÁ, Hans-Heiner GORRIS, Karel NOVOTNÝ, Petr SKLÁDAL, Jozef KAISER and Zdeněk FARKA. Laser-induced breakdown spectroscopy as a readout method for immunocytochemistry with upconversion nanoparticles. \textit{Microchimica Acta}. Springer-Verlag GmbH Austria, 2021, vol.~188, No~5, p.~''147'', 10 pp. ISSN~0026-3672. Available from: https://dx.doi.org/10.1007/s00604-021-04816-y.
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