Assessment of Antimicrobic, Antivirotic and Cytotoxic Potential
of Alginate Beads Cross-Linked by Bivalent Ions for Vaginal
Administration

J 2021

Assessment of Antimicrobic, Antivirotic and Cytotoxic Potential of Alginate Beads Cross-Linked by Bivalent Ions for Vaginal Administration

PAVELKOVÁ, Miroslava, Jakub VYSLOUŽIL, Kateřina KUBOVÁ, Sylvie PAVLOKOVÁ, D. MOLINKOVA et. al.

Basic information

Original name

Assessment of Antimicrobic, Antivirotic and Cytotoxic Potential of Alginate Beads Cross-Linked by Bivalent Ions for Vaginal Administration

Authors

PAVELKOVÁ, Miroslava (203 Czech Republic, belonging to the institution), Jakub VYSLOUŽIL (203 Czech Republic, guarantor, belonging to the institution), Kateřina KUBOVÁ (203 Czech Republic, belonging to the institution), Sylvie PAVLOKOVÁ (203 Czech Republic, belonging to the institution), D. MOLINKOVA (203 Czech Republic), V. CELER (203 Czech Republic), A. PECHOVA (203 Czech Republic), J. MASEK (203 Czech Republic) and David VETCHÝ (203 Czech Republic, belonging to the institution)

Edition

Pharmaceutics, BASEL, Elsevier, 2021, 0939-6411

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.589

RIV identification code

RIV/00216224:14160/21:00121389

Organization unit

Faculty of Pharmacy

DOI

http://dx.doi.org/10.3390/pharmaceutics13020165

UT WoS

000623016800001

Keywords in English

ionic gelation; alginate; cross-linking; bivalent ions; cytotoxicity; antimicrobial activity; antiviral activity

Abstract

V originále

Antimicrobial agent abuse poses a serious threat for future pharmacotherapy, including vaginal administration. The solution can be found in simple polymeric systems with inherent antimicrobial properties without the need to incorporate drugs, for instance alginate beads cross-linked by bivalent ions. The main goal of the presented study was to provide improvement on the well-documented cytotoxicity of Cu2+ cross-linked alginate. Alginate beads were prepared by external ionotropic gelation by cross-linking with Cu2+, Ca2+ and Zn2+ ions, separately and in mixtures. Morphological properties, swelling capacity, ion release and efficacy against the most common vaginal pathogens (C. albicans, E. coli, E. faecalis and virus strain-human herpesvirus type 1) were evaluated. The prepared particles (particle size 1455.68 +/- 18.71-1756.31 +/- 16.58 mu m) had very good sphericity (0.86 +/- 0.04-0.97 +/- 0.06). In mixture samples, Cu2+ hampered second ion loading, and was also released incompletely (18.75-44.8%) compared to the single ion Cu2+ sample (71.4%). Efficacy against the selected pathogens was confirmed in almost all samples. Although anticipating otherwise, ion mixture samples did not show betterment over a Cu2+ cross-linked sample in cytotoxicity-pathogen efficacy relation. However, the desired improvement was found in a single ion Zn2+ sample whose minimal inhibition concentrations against the pathogens (0.6-6.12 mM) were close to, or in the same mathematical order as, its toxic concentration of 50 (1.891 mM). In summary, these findings combined with alginate's biocompatibility and biodegradability give the combination solid potential in antimicrobial use.

Citovat

PAVELKOVÁ, Miroslava, Jakub VYSLOUŽIL, Kateřina KUBOVÁ, Sylvie PAVLOKOVÁ, D. MOLINKOVA, V. CELER, A. PECHOVA, J. MASEK and David VETCHÝ. Assessment of Antimicrobic, Antivirotic and Cytotoxic Potential of Alginate Beads Cross-Linked by Bivalent Ions for Vaginal Administration. Pharmaceutics. BASEL: Elsevier, 2021, vol. 13, No 2, p. 1-20. ISSN 0939-6411. Available from: https://dx.doi.org/10.3390/pharmaceutics13020165.

@article{1759161,
   author = {Pavelková, Miroslava and Vysloužil, Jakub and Kubová, Kateřina and Pavloková, Sylvie and Molinkova, D. and Celer, V. and Pechova, A. and Masek, J. and Vetchý, David},
   article_location = {BASEL},
   article_number = {2},
   doi = {http://dx.doi.org/10.3390/pharmaceutics13020165},
   keywords = {ionic gelation; alginate; cross-linking; bivalent ions; cytotoxicity; antimicrobial activity; antiviral activity},
   language = {eng},
   issn = {0939-6411},
   journal = {Pharmaceutics},
   title = {Assessment of Antimicrobic, Antivirotic and Cytotoxic Potential of Alginate Beads Cross-Linked by Bivalent Ions for Vaginal Administration},
   url = {https://pubmed.ncbi.nlm.nih.gov/33513747/},
   volume = {13},
   year = {2021}
}

TY  - JOUR
ID  - 1759161
AU  - Pavelková, Miroslava - Vysloužil, Jakub - Kubová, Kateřina - Pavloková, Sylvie - Molinkova, D. - Celer, V. - Pechova, A. - Masek, J. - Vetchý, David
PY  - 2021
TI  - Assessment of Antimicrobic, Antivirotic and Cytotoxic Potential of Alginate Beads Cross-Linked by Bivalent Ions for Vaginal Administration
JF  - Pharmaceutics
VL  - 13
IS  - 2
SP  - 1-20
EP  - 1-20
PB  - Elsevier
SN  - 09396411
KW  - ionic gelation
KW  - alginate
KW  - cross-linking
KW  - bivalent ions
KW  - cytotoxicity
KW  - antimicrobial activity
KW  - antiviral activity
UR  - https://pubmed.ncbi.nlm.nih.gov/33513747/
N2  - Antimicrobial agent abuse poses a serious threat for future pharmacotherapy, including vaginal administration. The solution can be found in simple polymeric systems with inherent antimicrobial properties without the need to incorporate drugs, for instance alginate beads cross-linked by bivalent ions. The main goal of the presented study was to provide improvement on the well-documented cytotoxicity of Cu2+ cross-linked alginate. Alginate beads were prepared by external ionotropic gelation by cross-linking with Cu2+, Ca2+ and Zn2+ ions, separately and in mixtures. Morphological properties, swelling capacity, ion release and efficacy against the most common vaginal pathogens (C. albicans, E. coli, E. faecalis and virus strain-human herpesvirus type 1) were evaluated. The prepared particles (particle size 1455.68 +/- 18.71-1756.31 +/- 16.58 mu m) had very good sphericity (0.86 +/- 0.04-0.97 +/- 0.06). In mixture samples, Cu2+ hampered second ion loading, and was also released incompletely (18.75-44.8%) compared to the single ion Cu2+ sample (71.4%). Efficacy against the selected pathogens was confirmed in almost all samples. Although anticipating otherwise, ion mixture samples did not show betterment over a Cu2+ cross-linked sample in cytotoxicity-pathogen efficacy relation. However, the desired improvement was found in a single ion Zn2+ sample whose minimal inhibition concentrations against the pathogens (0.6-6.12 mM) were close to, or in the same mathematical order as, its toxic concentration of 50 (1.891 mM). In summary, these findings combined with alginate's biocompatibility and biodegradability give the combination solid potential in antimicrobial use.
ER  -

PAVELKOVÁ, Miroslava, Jakub VYSLOUŽIL, Kateřina KUBOVÁ, Sylvie PAVLOKOVÁ, D. MOLINKOVA, V. CELER, A. PECHOVA, J. MASEK and David VETCHÝ. Assessment of Antimicrobic, Antivirotic and Cytotoxic Potential of Alginate Beads Cross-Linked by Bivalent Ions for Vaginal Administration. \textit{Pharmaceutics}. BASEL: Elsevier, 2021, vol.~13, No~2, p.~1-20. ISSN~0939-6411. Available from: https://dx.doi.org/10.3390/pharmaceutics13020165.

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