Urinary shedding of leptospires in palearctic bats

J 2021

Urinary shedding of leptospires in palearctic bats

SEIDLOVA, Veronika, Monika NEMCOVA, Jiří PIKULA, Tomáš BARTONIČKA, Astghik GHAZARYAN et. al.

Basic information

Original name

Urinary shedding of leptospires in palearctic bats

Authors

SEIDLOVA, Veronika (203 Czech Republic), Monika NEMCOVA (203 Czech Republic), Jiří PIKULA (203 Czech Republic, guarantor), Tomáš BARTONIČKA (203 Czech Republic, belonging to the institution), Astghik GHAZARYAN (51 Armenia), Tomas HEGER (203 Czech Republic), Tomasz KOKUREWICZ (616 Poland), Oleg L. ORLOV (643 Russian Federation), Sneha PATRA (203 Czech Republic), Vladimir PIACEK (203 Czech Republic), Frantisek TREML (203 Czech Republic), Katerina ZUKALOVA (203 Czech Republic) and Jan ZUKAL (203 Czech Republic, belonging to the institution)

Edition

Transboundary and Emerging Diseases, Hoboken, Wiley-Blackwell, 2021, 1865-1674

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.521

RIV identification code

RIV/00216224:14310/21:00121394

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1111/tbed.14011

UT WoS

000626853900001

Keywords in English

Chiroptera; genetic classification; non invasive sampling; pathogenic Leptospira; prevalence; reservoirs; urine

Abstract

V originále

Leptospirosis is a bacterial zoonotic infection of worldwide occurrence. Bats, like other mammalian reservoirs, may be long-term carriers that maintain endemicity of infection and shed viable leptospires in urine. Direct and/or indirect contact with these Leptospira shedders is the main risk factor as regards public health concern. However, knowledge about bat leptospirosis in the Palearctic Region, and in Europe in particular, is poor. We collected urine from 176 specimens of 11 bat species in the Czech Republic, Poland, Republic of Armenia and the Altai Region of Russia between 2014 and 2019. We extracted DNA from the urine samples to detect Leptospira spp. shedders using PCR amplification of the 16S rRNA and LipL32 genes. Four bat species (Barbastella barbastellus n = 1, Myotis bechsteinii n = 1, Myotis myotis n = 24 and Myotis nattereri n = 1) tested positive for Leptospira spp., with detected amplicons showing 100% genetic identity with pathogenic Leptospira interrogans. The site- and species-specific prevalence range was 0%-24.1% and 0%-20%, respectively. All bats sampled in the Republic of Armenia and Russia were negative. Given the circulation of pathogenic leptospires in strictly protected Palearctic bat species and their populations, non-invasive and non-lethal sampling of urine for molecular Leptospira spp. detection is recommended as a suitable surveillance and monitoring strategy. Moreover, our results should raise awareness of this potential disease risk among health professionals, veterinarians, chiropterologists and wildlife rescue workers handling bats, as well as speleologists and persons cleaning premises following bat infestation.

Citovat

SEIDLOVA, Veronika, Monika NEMCOVA, Jiří PIKULA, Tomáš BARTONIČKA, Astghik GHAZARYAN, Tomas HEGER, Tomasz KOKUREWICZ, Oleg L. ORLOV, Sneha PATRA, Vladimir PIACEK, Frantisek TREML, Katerina ZUKALOVA and Jan ZUKAL. Urinary shedding of leptospires in palearctic bats. Transboundary and Emerging Diseases. Hoboken: Wiley-Blackwell, 2021, vol. 68, No 6, p. 3089-3095. ISSN 1865-1674. Available from: https://dx.doi.org/10.1111/tbed.14011.

@article{1759380,
   author = {Seidlova, Veronika and Nemcova, Monika and Pikula, Jiří and Bartonička, Tomáš and Ghazaryan, Astghik and Heger, Tomas and Kokurewicz, Tomasz and Orlov, Oleg L. and Patra, Sneha and Piacek, Vladimir and Treml, Frantisek and Zukalova, Katerina and Zukal, Jan},
   article_location = {Hoboken},
   article_number = {6},
   doi = {http://dx.doi.org/10.1111/tbed.14011},
   keywords = {Chiroptera; genetic classification; non invasive sampling; pathogenic Leptospira; prevalence; reservoirs; urine},
   language = {eng},
   issn = {1865-1674},
   journal = {Transboundary and Emerging Diseases},
   title = {Urinary shedding of leptospires in palearctic bats},
   url = {https://doi.org/10.1111/tbed.14011},
   volume = {68},
   year = {2021}
}

TY  - JOUR
ID  - 1759380
AU  - Seidlova, Veronika - Nemcova, Monika - Pikula, Jiří - Bartonička, Tomáš - Ghazaryan, Astghik - Heger, Tomas - Kokurewicz, Tomasz - Orlov, Oleg L. - Patra, Sneha - Piacek, Vladimir - Treml, Frantisek - Zukalova, Katerina - Zukal, Jan
PY  - 2021
TI  - Urinary shedding of leptospires in palearctic bats
JF  - Transboundary and Emerging Diseases
VL  - 68
IS  - 6
SP  - 3089-3095
EP  - 3089-3095
PB  - Wiley-Blackwell
SN  - 18651674
KW  - Chiroptera
KW  - genetic classification
KW  - non invasive sampling
KW  - pathogenic Leptospira
KW  - prevalence
KW  - reservoirs
KW  - urine
UR  - https://doi.org/10.1111/tbed.14011
N2  - Leptospirosis is a bacterial zoonotic infection of worldwide occurrence. Bats, like other mammalian reservoirs, may be long-term carriers that maintain endemicity of infection and shed viable leptospires in urine. Direct and/or indirect contact with these Leptospira shedders is the main risk factor as regards public health concern. However, knowledge about bat leptospirosis in the Palearctic Region, and in Europe in particular, is poor. We collected urine from 176 specimens of 11 bat species in the Czech Republic, Poland, Republic of Armenia and the Altai Region of Russia between 2014 and 2019. We extracted DNA from the urine samples to detect Leptospira spp. shedders using PCR amplification of the 16S rRNA and LipL32 genes. Four bat species (Barbastella barbastellus n = 1, Myotis bechsteinii n = 1, Myotis myotis n = 24 and Myotis nattereri n = 1) tested positive for Leptospira spp., with detected amplicons showing 100% genetic identity with pathogenic Leptospira interrogans. The site- and species-specific prevalence range was 0%-24.1% and 0%-20%, respectively. All bats sampled in the Republic of Armenia and Russia were negative. Given the circulation of pathogenic leptospires in strictly protected Palearctic bat species and their populations, non-invasive and non-lethal sampling of urine for molecular Leptospira spp. detection is recommended as a suitable surveillance and monitoring strategy. Moreover, our results should raise awareness of this potential disease risk among health professionals, veterinarians, chiropterologists and wildlife rescue workers handling bats, as well as speleologists and persons cleaning premises following bat infestation.
ER  -

SEIDLOVA, Veronika, Monika NEMCOVA, Jiří PIKULA, Tomáš BARTONIČKA, Astghik GHAZARYAN, Tomas HEGER, Tomasz KOKUREWICZ, Oleg L. ORLOV, Sneha PATRA, Vladimir PIACEK, Frantisek TREML, Katerina ZUKALOVA and Jan ZUKAL. Urinary shedding of leptospires in palearctic bats. \textit{Transboundary and Emerging Diseases}. Hoboken: Wiley-Blackwell, 2021, vol.~68, No~6, p.~3089-3095. ISSN~1865-1674. Available from: https://dx.doi.org/10.1111/tbed.14011.

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