Unique organization of photosystem II supercomplexes and
megacomplexes in Norway spruce

J 2020

Unique organization of photosystem II supercomplexes and megacomplexes in Norway spruce

KOURIL, R., L. NOSEK, M. OPATIKOVA, R. ARSHAD, D.A. SEMCHONOK et. al.

Basic information

Original name

Unique organization of photosystem II supercomplexes and megacomplexes in Norway spruce

Authors

KOURIL, R., L. NOSEK, M. OPATIKOVA, R. ARSHAD, D.A. SEMCHONOK, I. CHAMRAD, R. LENOBEL, E.J. BOEKEMA and P. ILIK

Edition

Plant Journal, Hoboken, Wiley, 2020, 0960-7412

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.417

RIV identification code

RIV/00216224:14740/20:00121451

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1111/tpj.14918

UT WoS

000557081500001

Keywords in English

clear native polyacrylamide electrophoresis; Picea abies; Pinus sylvestris; photosystem II; megacomplex; supercomplex; single-particle electron microscopy; grana membrane

Abstract

V originále

Photosystem II (PSII) complexes are organized into large supercomplexes with variable amounts of light-harvesting proteins (Lhcb). A typical PSII supercomplex in plants is formed by four trimers of Lhcb proteins (LHCII trimers), which are bound to the PSII core dimer via monomeric antenna proteins. However, the architecture of PSII supercomplexes in Norway spruce[Picea abies(L.) Karst.] is different, most likely due to a lack of two Lhcb proteins, Lhcb6 and Lhcb3. Interestingly, the spruce PSII supercomplex shares similar structural features with its counterpart in the green algaChlamydomonas reinhardtii[Kourilet al. (2016)New Phytol.210, 808-814]. Here we present a single-particle electron microscopy study of isolated PSII supercomplexes from Norway spruce that revealed binding of a variable amount of LHCII trimers to the PSII core dimer at positions that have never been observed in any other plant species so far. The largest spruce PSII supercomplex, which was found to bind eight LHCII trimers, is even larger than the current largest known PSII supercomplex fromC. reinhardtii. We have also shown that the spruce PSII supercomplexes can form various types of PSII megacomplexes, which were also identified in intact grana membranes. Some of these large PSII supercomplexes and megacomplexes were identified also inPinus sylvestris, another representative of the Pinaceae family. The structural variability and complexity of LHCII organization in Pinaceae seems to be related to the absence of Lhcb6 and Lhcb3 in this family, and may be beneficial for the optimization of light-harvesting under varying environmental conditions.

Links

90043, large research infrastructures

Name: CIISB

Citovat

KOURIL, R., L. NOSEK, M. OPATIKOVA, R. ARSHAD, D.A. SEMCHONOK, I. CHAMRAD, R. LENOBEL, E.J. BOEKEMA and P. ILIK. Unique organization of photosystem II supercomplexes and megacomplexes in Norway spruce. Plant Journal. Hoboken: Wiley, 2020, vol. 104, No 1, p. 215-225. ISSN 0960-7412. Available from: https://dx.doi.org/10.1111/tpj.14918.

@article{1762517,
   author = {Kouril, R. and Nosek, L. and Opatikova, M. and Arshad, R. and Semchonok, D.A. and Chamrad, I. and Lenobel, R. and Boekema, E.J. and Ilik, P.},
   article_location = {Hoboken},
   article_number = {1},
   doi = {http://dx.doi.org/10.1111/tpj.14918},
   keywords = {clear native polyacrylamide electrophoresis; Picea abies; Pinus sylvestris; photosystem II; megacomplex; supercomplex; single-particle electron microscopy; grana membrane},
   language = {eng},
   issn = {0960-7412},
   journal = {Plant Journal},
   title = {Unique organization of photosystem II supercomplexes and megacomplexes in Norway spruce},
   url = {https://onlinelibrary.wiley.com/doi/10.1111/tpj.14918},
   volume = {104},
   year = {2020}
}

TY  - JOUR
ID  - 1762517
AU  - Kouril, R. - Nosek, L. - Opatikova, M. - Arshad, R. - Semchonok, D.A. - Chamrad, I. - Lenobel, R. - Boekema, E.J. - Ilik, P.
PY  - 2020
TI  - Unique organization of photosystem II supercomplexes and megacomplexes in Norway spruce
JF  - Plant Journal
VL  - 104
IS  - 1
SP  - 215-225
EP  - 215-225
PB  - Wiley
SN  - 09607412
KW  - clear native polyacrylamide electrophoresis
KW  - Picea abies
KW  - Pinus sylvestris
KW  - photosystem II
KW  - megacomplex
KW  - supercomplex
KW  - single-particle electron microscopy
KW  - grana membrane
UR  - https://onlinelibrary.wiley.com/doi/10.1111/tpj.14918
N2  - Photosystem II (PSII) complexes are organized into large supercomplexes with variable amounts of light-harvesting proteins (Lhcb). A typical PSII supercomplex in plants is formed by four trimers of Lhcb proteins (LHCII trimers), which are bound to the PSII core dimer via monomeric antenna proteins. However, the architecture of PSII supercomplexes in Norway spruce[Picea abies(L.) Karst.] is different, most likely due to a lack of two Lhcb proteins, Lhcb6 and Lhcb3. Interestingly, the spruce PSII supercomplex shares similar structural features with its counterpart in the green algaChlamydomonas reinhardtii[Kourilet al. (2016)New Phytol.210, 808-814]. Here we present a single-particle electron microscopy study of isolated PSII supercomplexes from Norway spruce that revealed binding of a variable amount of LHCII trimers to the PSII core dimer at positions that have never been observed in any other plant species so far. The largest spruce PSII supercomplex, which was found to bind eight LHCII trimers, is even larger than the current largest known PSII supercomplex fromC. reinhardtii. We have also shown that the spruce PSII supercomplexes can form various types of PSII megacomplexes, which were also identified in intact grana membranes. Some of these large PSII supercomplexes and megacomplexes were identified also inPinus sylvestris, another representative of the Pinaceae family. The structural variability and complexity of LHCII organization in Pinaceae seems to be related to the absence of Lhcb6 and Lhcb3 in this family, and may be beneficial for the optimization of light-harvesting under varying environmental conditions.
ER  -

KOURIL, R., L. NOSEK, M. OPATIKOVA, R. ARSHAD, D.A. SEMCHONOK, I. CHAMRAD, R. LENOBEL, E.J. BOEKEMA and P. ILIK. Unique organization of photosystem II supercomplexes and megacomplexes in Norway spruce. \textit{Plant Journal}. Hoboken: Wiley, 2020, vol.~104, No~1, p.~215-225. ISSN~0960-7412. Available from: https://dx.doi.org/10.1111/tpj.14918.

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