Detailed Information on Publication Record
2020
Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing
KUDR, J., L. ZHAO, E.P. NGUYEN, H. AROLA, T.K. NEVANEN et. al.Basic information
Original name
Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing
Authors
KUDR, J., L. ZHAO, E.P. NGUYEN, H. AROLA, T.K. NEVANEN, V. ADAM, O. ZITKA and A. MERKOCI
Edition
BIOSENSORS & BIOELECTRONICS, OXFORD, ELSEVIER ADVANCED TECHNOLOGY, 2020, 0956-5663
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10403 Physical chemistry
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 10.618
RIV identification code
RIV/00216224:14740/20:00121453
Organization unit
Central European Institute of Technology
UT WoS
000523557300005
Keywords in English
2D material; Antibody; Biosensor; Electrochemistry; Graphene oxide
Tags
International impact, Reviewed
Změněno: 29/4/2021 12:23, Mgr. Pavla Foltynová, Ph.D.
Abstract
V originále
The design and application of an inkjet-printed electrochemically reduced graphene oxide microelectrode for HT-2 mycotoxin immunoenzymatic biosensing is reported. A water-based graphene oxide ink was first formulated and single-drop line working microelectrodes were inkjet-printed onto poly(ethylene 2,6-naphthalate) substrates, with dimensions of 78 mu m in width and 30 nm in height after solvent evaporation. The printed graphene oxide microelectmdes were electrochemically reduced and characterized by Raman and X-ray photoelectron spectroscopies in addition to microscopies. Through optimization of the electrochemical reduction parameters, differential pulse voltammetry were performed to examine the sensing of 1-naphthol (1-N), where it was revealed that reduction times had significant effects on electrode performance. The developed microelectrodes were then used as an immunoenzymatic biosensor for the detection of HT-2 mycotoxin based on carbodiimide linking of the microelectmde surface and HT-2 toxin antigen binding fragment of antibody (anti-HT2 (10) Fab). The HT-2 toxin and anti-HT2 (10) Fab reaction was reported by anti-HT2 immune complex single-chain variable fragment of antibody fused with alkaline phosphatase (anti-IC-HT2 scFv-ALP) which is able to produce an electmactive reporter - 1-N. The biosensor showed detection limit of 1.6 ng . mL(-1) and a linear dynamic range of 6.3 - 100.0 ng . mL(-1) within a 5 min incubation with 1-naphthyl phosphate (1-NP) substrate.
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