KUDR, J., L. ZHAO, E.P. NGUYEN, H. AROLA, T.K. NEVANEN, V. ADAM, O. ZITKA and A. MERKOCI. Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing. BIOSENSORS & BIOELECTRONICS. OXFORD: ELSEVIER ADVANCED TECHNOLOGY, 2020, vol. 156, MAY, p. 112109-112116. ISSN 0956-5663. Available from: https://dx.doi.org/10.1016/j.bios.2020.112109. |
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@article{1762519, author = {Kudr, J. and Zhao, L. and Nguyen, E.P. and Arola, H. and Nevanen, T.K. and Adam, V. and Zitka, O. and Merkoci, A.}, article_location = {OXFORD}, article_number = {MAY}, doi = {http://dx.doi.org/10.1016/j.bios.2020.112109}, keywords = {2D material; Antibody; Biosensor; Electrochemistry; Graphene oxide}, language = {eng}, issn = {0956-5663}, journal = {BIOSENSORS & BIOELECTRONICS}, title = {Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing}, url = {https://www.sciencedirect.com/science/article/pii/S0956566320301068?via%3Dihub}, volume = {156}, year = {2020} }
TY - JOUR ID - 1762519 AU - Kudr, J. - Zhao, L. - Nguyen, E.P. - Arola, H. - Nevanen, T.K. - Adam, V. - Zitka, O. - Merkoci, A. PY - 2020 TI - Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing JF - BIOSENSORS & BIOELECTRONICS VL - 156 IS - MAY SP - 112109 EP - 112109 PB - ELSEVIER ADVANCED TECHNOLOGY SN - 09565663 KW - 2D material KW - Antibody KW - Biosensor KW - Electrochemistry KW - Graphene oxide UR - https://www.sciencedirect.com/science/article/pii/S0956566320301068?via%3Dihub N2 - The design and application of an inkjet-printed electrochemically reduced graphene oxide microelectrode for HT-2 mycotoxin immunoenzymatic biosensing is reported. A water-based graphene oxide ink was first formulated and single-drop line working microelectrodes were inkjet-printed onto poly(ethylene 2,6-naphthalate) substrates, with dimensions of 78 mu m in width and 30 nm in height after solvent evaporation. The printed graphene oxide microelectmdes were electrochemically reduced and characterized by Raman and X-ray photoelectron spectroscopies in addition to microscopies. Through optimization of the electrochemical reduction parameters, differential pulse voltammetry were performed to examine the sensing of 1-naphthol (1-N), where it was revealed that reduction times had significant effects on electrode performance. The developed microelectrodes were then used as an immunoenzymatic biosensor for the detection of HT-2 mycotoxin based on carbodiimide linking of the microelectmde surface and HT-2 toxin antigen binding fragment of antibody (anti-HT2 (10) Fab). The HT-2 toxin and anti-HT2 (10) Fab reaction was reported by anti-HT2 immune complex single-chain variable fragment of antibody fused with alkaline phosphatase (anti-IC-HT2 scFv-ALP) which is able to produce an electmactive reporter - 1-N. The biosensor showed detection limit of 1.6 ng . mL(-1) and a linear dynamic range of 6.3 - 100.0 ng . mL(-1) within a 5 min incubation with 1-naphthyl phosphate (1-NP) substrate. ER -
KUDR, J., L. ZHAO, E.P. NGUYEN, H. AROLA, T.K. NEVANEN, V. ADAM, O. ZITKA and A. MERKOCI. Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing. \textit{BIOSENSORS \&{}amp; BIOELECTRONICS}. OXFORD: ELSEVIER ADVANCED TECHNOLOGY, 2020, vol.~156, MAY, p.~112109-112116. ISSN~0956-5663. Available from: https://dx.doi.org/10.1016/j.bios.2020.112109.
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