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@article{1762523, author = {Stojanovic, Z.S. and Durovic, A.D. and Ashrafi, A.M. and Koudelkova, Z. and Zitka, O. and Richtera, L.}, article_location = {Lausanne}, article_number = {SEP}, doi = {http://dx.doi.org/10.1016/j.snb.2020.128141}, keywords = {GSH; GSSG; Simultaneous determination; Modified carbon paste electrode; Square-wave voltammetry; Urine}, language = {eng}, issn = {0925-4005}, journal = {Sensors and Actuators B: Chemical}, title = {Highly sensitive simultaneous electrochemical determination of reduced and oxidized glutathione in urine samples using antimony trioxide modified carbon paste electrode}, url = {https://www.sciencedirect.com/science/article/pii/S0925400520304913?via%3Dihub}, volume = {318}, year = {2020} }
TY - JOUR ID - 1762523 AU - Stojanovic, Z.S. - Durovic, A.D. - Ashrafi, A.M. - Koudelkova, Z. - Zitka, O. - Richtera, L. PY - 2020 TI - Highly sensitive simultaneous electrochemical determination of reduced and oxidized glutathione in urine samples using antimony trioxide modified carbon paste electrode JF - Sensors and Actuators B: Chemical VL - 318 IS - SEP SP - 128141 EP - 128141 PB - Elsevier SN - 09254005 KW - GSH KW - GSSG KW - Simultaneous determination KW - Modified carbon paste electrode KW - Square-wave voltammetry KW - Urine UR - https://www.sciencedirect.com/science/article/pii/S0925400520304913?via%3Dihub N2 - A simple and reproducible electroanalytical sensor using antimony trioxide modified-carbon paste electrode (Sb2O3-CPE) as sensing element was employed for the first time for simultaneous determination of glutathione (GSH) and glutathione disulphide (GSSG). The electrochemical response of Sb2O3-CPE was characterized by cyclic voltammetry and square-wave voltammetry. Scanning electron microscopy (SEM), scanning electrochemical microscopy (SECM) and Raman mapping were used to characterize the morphology and surface modification. A significant enhancement in the peak current response of GSH and GSSG accompanied with a good separation of the peaks were obtained at the modified electrode compared to the bare CPE. GSH and GSSG exhibited well-defined oxidation peaks at +1.08 V and +1.36 V (vs. Ag/AgCl, 3 mol/L) in Britton-Robinson buffer at pH 6.0. Parameters such as used supporting electrolyte and its pH value, amplitude and frequency were optimized for square-wave voltammetric determination. Under optimal conditions, voltammetric responses were linear in the concentration range of 2-200 mu mol/L for GSH and GSSG. The limits of detection were 0.34 mu mol/L and 0.10 mu mol/L for GSH and GSSG, respectively. Moreover, the proposed sensor also showed good reproducibility, stability and selectivity. Finally, the proposed sensor was applied for the simultaneous determination of GSH and GSSG in urine samples with satisfactory results. ER -
STOJANOVIC, Z.S., A.D. DUROVIC, A.M. ASHRAFI, Z. KOUDELKOVA, O. ZITKA a L. RICHTERA. Highly sensitive simultaneous electrochemical determination of reduced and oxidized glutathione in urine samples using antimony trioxide modified carbon paste electrode. \textit{Sensors and Actuators B: Chemical}. Lausanne: Elsevier, 2020, roč.~318, SEP, s.~128141-128152. ISSN~0925-4005. Dostupné z: https://dx.doi.org/10.1016/j.snb.2020.128141.
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