| HANKEOVÁ, Simona, Jakub ŠALPLACHTA, Tomáš ZIKMUND, Michaela KAVKOVÁ, Noemi VAN HUL, Adam BŘÍNEK, Veronika SMÉKALOVÁ, Jakub LÁZŇOVSKÝ, Feven DAWIT, Josef JAROŠ, Vítězslav BRYJA, Urban LENDAHL, Ewa ELLIS, Antal NEMETH, Bjorn FISCHLER, Edouard HANNEZO, Jozef KAISER and Emma Rachel ANDERSSON. DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome. eLife. eLife Sciences Publications Ltd, 2021, vol. 10, February, p. 1-29. ISSN 2050-084X. Available from: https://dx.doi.org/10.7554/eLife.60916. |
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@article{1765796,
author = {Hankeová, Simona and Šalplachta, Jakub and Zikmund, Tomáš and Kavková, Michaela and Van Hul, Noemi and Břínek, Adam and Smékalová, Veronika and Lázňovský, Jakub and Dawit, Feven and Jaroš, Josef and Bryja, Vítězslav and Lendahl, Urban and Ellis, Ewa and Nemeth, Antal and Fischler, Bjorn and Hannezo, Edouard and Kaiser, Jozef and Andersson, Emma Rachel},
article_number = {February},
doi = {http://dx.doi.org/10.7554/eLife.60916},
keywords = {PORTAL-VEIN; ARTERIOHEPATIC DYSPLASIA; HEPATIC-ARTERY; HUMAN JAGGED1; LIVER; MUTATIONS; INSIGHTS; NETWORK; ORGAN},
language = {eng},
issn = {2050-084X},
journal = {eLife},
title = {DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome},
url = {https://elifesciences.org/articles/60916#xb0ffa41b},
volume = {10},
year = {2021}
}
TY - JOUR
ID - 1765796
AU - Hankeová, Simona - Šalplachta, Jakub - Zikmund, Tomáš - Kavková, Michaela - Van Hul, Noemi - Břínek, Adam - Smékalová, Veronika - Lázňovský, Jakub - Dawit, Feven - Jaroš, Josef - Bryja, Vítězslav - Lendahl, Urban - Ellis, Ewa - Nemeth, Antal - Fischler, Bjorn - Hannezo, Edouard - Kaiser, Jozef - Andersson, Emma Rachel
PY - 2021
TI - DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome
JF - eLife
VL - 10
IS - February
SP - 1-29
EP - 1-29
PB - eLife Sciences Publications Ltd
SN - 2050084X
KW - PORTAL-VEIN
KW - ARTERIOHEPATIC DYSPLASIA
KW - HEPATIC-ARTERY
KW - HUMAN JAGGED1
KW - LIVER
KW - MUTATIONS
KW - INSIGHTS
KW - NETWORK
KW - ORGAN
UR - https://elifesciences.org/articles/60916#xb0ffa41b
N2 - Organ function depends on tissues adopting the correct architecture. However, insights into organ architecture are currently hampered by an absence of standardized quantitative 3D analysis. We aimed to develop a robust technology to visualize, digitalize, and segment the architecture of two tubular systems in 3D: double resin casting micro computed tomography (DUCT). As proof of principle, we applied DUCT to a mouse model for Alagille syndrome (Jag1(Ndr/Ndr) mice), characterized by intrahepatic bile duct paucity, that can spontaneously generate a biliary system in adulthood. DUCT identified increased central biliary branching and peripheral bile duct tortuosity as two compensatory processes occurring in distinct regions of Jag1(Ndr/Ndr) liver, leading to full reconstitution of wild-type biliary volume and phenotypic recovery. DUCT is thus a powerful new technology for 3D analysis, which can reveal novel phenotypes and provide a standardized method of defining liver architecture in mouse models.
ER -
HANKEOVÁ, Simona, Jakub ŠALPLACHTA, Tomáš ZIKMUND, Michaela KAVKOVÁ, Noemi VAN HUL, Adam BŘÍNEK, Veronika SMÉKALOVÁ, Jakub LÁZŇOVSKÝ, Feven DAWIT, Josef JAROŠ, Vítězslav BRYJA, Urban LENDAHL, Ewa ELLIS, Antal NEMETH, Bjorn FISCHLER, Edouard HANNEZO, Jozef KAISER and Emma Rachel ANDERSSON. DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome. \textit{eLife}. eLife Sciences Publications Ltd, 2021, vol.~10, February, p.~1-29. ISSN~2050-084X. Available from: https://dx.doi.org/10.7554/eLife.60916.
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