Measurement of respiration and acidification rates of mammalian
cells in thermoplastic microfluidic devices

J 2021

Measurement of respiration and acidification rates of mammalian cells in thermoplastic microfluidic devices

MÜLLER, Bernhard; Philipp SULZER; Manuel WALCH; Helene ZIRATH; Tomáš BURYŠKA et al.

Základní údaje

Originální název

Measurement of respiration and acidification rates of mammalian cells in thermoplastic microfluidic devices

Autoři

MÜLLER, Bernhard; Philipp SULZER; Manuel WALCH; Helene ZIRATH; Tomáš BURYŠKA; Mario ROTHBAUER; Peter ERTL a Torsten MAYR

Vydání

Sensors and Actuators B: Chemical, Elsevier B.V. 2021, 0925-4005

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 9.221

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00121562

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Luminescent optical sensor; Oxygen pO2; pH; Online-monitoring; Perfused cell culture; Metabolism

Štítky

14314010, 143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 5. 2021 09:54, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Luminescent chemical sensors have been proven to be a valuable asset in cell cultures and generally in microbiological studies. We present microfluidic cell culture devices with integrated optical chemical sensors. The integrated sensors offer the possibility to monitor dissolved oxygen and pH levels and enable the measurement of respiration and acidification rates. The cell metabolism was observed using this method by temporarily stopping the incubation flow, barely influencing the cell culture. A thermoplastic polymer is used as oxygen impermeable chip material to enable the measurement of respiration rates, which is not possible with widely used PDMS-based microfluidics. Hence, an innovative fast prototyping strategy is utilized to enable the replication of small series of thermoplastic microfluidic chips. We were able to demonstrate the suitability of this measurement method by monitoring the metabolic response of a human lung carcinoma epithelial-like cell line (A549) to the exposure of FCCP (Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone), a drug known for its up-regulating effect on respiration and acidification rates. This universal measurement approach can potentially be deployed in all sorts of microfluidic devices and help to retrieve valuable data from inside those systems beyond classical end-point detection methods. It therefore complements modern 3D cell cultures and organ-on-chip research with a powerful analytical technology for gathering information about ongoing cell metabolism.

Citovat

MÜLLER, Bernhard; Philipp SULZER; Manuel WALCH; Helene ZIRATH; Tomáš BURYŠKA; Mario ROTHBAUER; Peter ERTL a Torsten MAYR. Measurement of respiration and acidification rates of mammalian cells in thermoplastic microfluidic devices. Sensors and Actuators B: Chemical. Elsevier B.V., 2021, roč. 334, May, s. "129664", 9 s. ISSN 0925-4005. Dostupné z: https://doi.org/10.1016/j.snb.2021.129664.

@article{1767356,
   author = {Müller, Bernhard and Sulzer, Philipp and Walch, Manuel and Zirath, Helene and Buryška, Tomáš and Rothbauer, Mario and Ertl, Peter and Mayr, Torsten},
   article_number = {May},
   doi = {https://doi.org/10.1016/j.snb.2021.129664},
   keywords = {Luminescent optical sensor; Oxygen pO2; pH; Online-monitoring; Perfused cell culture; Metabolism},
   language = {eng},
   issn = {0925-4005},
   journal = {Sensors and Actuators B: Chemical},
   title = {Measurement of respiration and acidification rates of mammalian cells in thermoplastic microfluidic devices},
   url = {https://www.sciencedirect.com/science/article/pii/S092540052100232X#!},
   volume = {334},
   year = {2021}
}

TY  - JOUR
ID  - 1767356
AU  - Müller, Bernhard - Sulzer, Philipp - Walch, Manuel - Zirath, Helene - Buryška, Tomáš - Rothbauer, Mario - Ertl, Peter - Mayr, Torsten
PY  - 2021
TI  - Measurement of respiration and acidification rates of mammalian cells in thermoplastic microfluidic devices
JF  - Sensors and Actuators B: Chemical
VL  - 334
IS  - May
SP  - "129664"
EP  - "129664"
PB  - Elsevier B.V.
SN  - 09254005
KW  - Luminescent optical sensor
KW  - Oxygen pO2
KW  - pH
KW  - Online-monitoring
KW  - Perfused cell culture
KW  - Metabolism
UR  - https://www.sciencedirect.com/science/article/pii/S092540052100232X#!
N2  - Luminescent chemical sensors have been proven to be a valuable asset in cell cultures and generally in microbiological studies. We present microfluidic cell culture devices with integrated optical chemical sensors. The integrated sensors offer the possibility to monitor dissolved oxygen and pH levels and enable the measurement of respiration and acidification rates. The cell metabolism was observed using this method by temporarily stopping the incubation flow, barely influencing the cell culture. A thermoplastic polymer is used as oxygen impermeable chip material to enable the measurement of respiration rates, which is not possible with widely used PDMS-based microfluidics. Hence, an innovative fast prototyping strategy is utilized to enable the replication of small series of thermoplastic microfluidic chips. We were able to demonstrate the suitability of this measurement method by monitoring the metabolic response of a human lung carcinoma epithelial-like cell line (A549) to the exposure of FCCP (Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone), a drug known for its up-regulating effect on respiration and acidification rates. This universal measurement approach can potentially be deployed in all sorts of microfluidic devices and help to retrieve valuable data from inside those systems beyond classical end-point detection methods. It therefore complements modern 3D cell cultures and organ-on-chip research with a powerful analytical technology for gathering information about ongoing cell metabolism.
ER  -

MÜLLER, Bernhard; Philipp SULZER; Manuel WALCH; Helene ZIRATH; Tomáš BURYŠKA; Mario ROTHBAUER; Peter ERTL a Torsten MAYR. Measurement of respiration and acidification rates of mammalian cells in thermoplastic microfluidic devices. \textit{Sensors and Actuators B: Chemical}. Elsevier B.V., 2021, roč.~334, May, s.~''129664'', 9 s. ISSN~0925-4005. Dostupné z: https://doi.org/10.1016/j.snb.2021.129664.

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