The effect of different types of lactose monohydrate on the
stability of acetylcholinesterase immobilized on carriers
designed to detect nerve agents

J 2021

The effect of different types of lactose monohydrate on the stability of acetylcholinesterase immobilized on carriers designed to detect nerve agents

ZEMAN, Jiří, Sylvie PAVLOKOVÁ, David VETCHÝ and Vladimír PITSCHMANN

Basic information

Original name

The effect of different types of lactose monohydrate on the stability of acetylcholinesterase immobilized on carriers designed to detect nerve agents

Authors

ZEMAN, Jiří (203 Czech Republic, belonging to the institution), Sylvie PAVLOKOVÁ (203 Czech Republic, belonging to the institution), David VETCHÝ (203 Czech Republic, guarantor, belonging to the institution) and Vladimír PITSCHMANN (203 Czech Republic)

Edition

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, HOBOKEN, NJ USA, WILEY, 2021, 0268-2575

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.709

RIV identification code

RIV/00216224:14160/21:00120053

Organization unit

Faculty of Pharmacy

DOI

http://dx.doi.org/10.1002/jctb.6700

UT WoS

000625465600001

Keywords in English

acetylcholinesterase; detection tube; Ellman' s reaction; lactose; soman; stability

Abstract

V originále

BACKGROUND Maintaining the stability of an immobilized enzyme during storage is generally very problematic. However, various substances have been reported to have a positive effect on enzyme stability. These include reducing disaccharide lactose, which is used in carriers in the form of pellets with immobilized cholinesterase. These carriers serve as a filling for detection tubes (biosensor), which are used for detecting nerve agents, wherein the detection itself is based on a colorimetric Ellman's reaction. To date, however, no study has been performed on the effect of different types of lactose monohydrate on the properties of such carriers. RESULTS The effect of five different types of lactose monohydrate on carrier properties and enzyme stability was examined. The lactose powders used differed in the particle size distribution, shape, compactability and flowability. The carriers were prepared in triplicate and subjected to a 24-month stability test under three storage conditions, with the results being statistically analyzed by analysis of variance, principal component analysis and t-test. Samples containing lactose with spherical particles were more resistant to degradation by heat and moisture than samples containing crystalline particles; in addition they had higher hardness and better sensitivity. All samples were also tested with soman after 24 months of storage and were found to retain very good sensitivity. CONCLUSION Samples containing spherical types of lactose proved to be more stable in some critical parameters compared to the samples with lactose in the form of single crystals, which are currently used in commercially available detection tubes. (c) 2021 Society of Chemical Industry (SCI).

Links

VI20192022172, research and development project

Name: Detekční trubičky druhé generace pro kontinuální a opakované monitorování nervově paralytických a jiných toxických látek

Investor: Ministry of the Interior of the CR

Citovat

ZEMAN, Jiří, Sylvie PAVLOKOVÁ, David VETCHÝ and Vladimír PITSCHMANN. The effect of different types of lactose monohydrate on the stability of acetylcholinesterase immobilized on carriers designed to detect nerve agents. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY. HOBOKEN, NJ USA: WILEY, 2021, vol. 96, No 6, p. 1758-1769. ISSN 0268-2575. Available from: https://dx.doi.org/10.1002/jctb.6700.

@article{1768383,
   author = {Zeman, Jiří and Pavloková, Sylvie and Vetchý, David and Pitschmann, Vladimír},
   article_location = {HOBOKEN, NJ USA},
   article_number = {6},
   doi = {http://dx.doi.org/10.1002/jctb.6700},
   keywords = {acetylcholinesterase; detection tube; Ellman' s reaction; lactose; soman; stability},
   language = {eng},
   issn = {0268-2575},
   journal = {JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY},
   title = {The effect of different types of lactose monohydrate on the stability of acetylcholinesterase immobilized on carriers designed to detect nerve agents},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/jctb.6700},
   volume = {96},
   year = {2021}
}

TY  - JOUR
ID  - 1768383
AU  - Zeman, Jiří - Pavloková, Sylvie - Vetchý, David - Pitschmann, Vladimír
PY  - 2021
TI  - The effect of different types of lactose monohydrate on the stability of acetylcholinesterase immobilized on carriers designed to detect nerve agents
JF  - JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
VL  - 96
IS  - 6
SP  - 1758-1769
EP  - 1758-1769
PB  - WILEY
SN  - 02682575
KW  - acetylcholinesterase
KW  - detection tube
KW  - Ellman' s reaction
KW  - lactose
KW  - soman
KW  - stability
UR  - https://onlinelibrary.wiley.com/doi/10.1002/jctb.6700
N2  - BACKGROUND Maintaining the stability of an immobilized enzyme during storage is generally very problematic. However, various substances have been reported to have a positive effect on enzyme stability. These include reducing disaccharide lactose, which is used in carriers in the form of pellets with immobilized cholinesterase. These carriers serve as a filling for detection tubes (biosensor), which are used for detecting nerve agents, wherein the detection itself is based on a colorimetric Ellman's reaction. To date, however, no study has been performed on the effect of different types of lactose monohydrate on the properties of such carriers. RESULTS The effect of five different types of lactose monohydrate on carrier properties and enzyme stability was examined. The lactose powders used differed in the particle size distribution, shape, compactability and flowability. The carriers were prepared in triplicate and subjected to a 24-month stability test under three storage conditions, with the results being statistically analyzed by analysis of variance, principal component analysis and t-test. Samples containing lactose with spherical particles were more resistant to degradation by heat and moisture than samples containing crystalline particles; in addition they had higher hardness and better sensitivity. All samples were also tested with soman after 24 months of storage and were found to retain very good sensitivity. CONCLUSION Samples containing spherical types of lactose proved to be more stable in some critical parameters compared to the samples with lactose in the form of single crystals, which are currently used in commercially available detection tubes. (c) 2021 Society of Chemical Industry (SCI).
ER  -

ZEMAN, Jiří, Sylvie PAVLOKOVÁ, David VETCHÝ and Vladimír PITSCHMANN. The effect of different types of lactose monohydrate on the stability of acetylcholinesterase immobilized on carriers designed to detect nerve agents. \textit{JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY}. HOBOKEN, NJ USA: WILEY, 2021, vol.~96, No~6, p.~1758-1769. ISSN~0268-2575. Available from: https://dx.doi.org/10.1002/jctb.6700.

Detailed Information on Publication Record