ZEMAN, Jiří, Sylvie PAVLOKOVÁ, David VETCHÝ a Vladimír PITSCHMANN. The effect of different types of lactose monohydrate on the stability of acetylcholinesterase immobilized on carriers designed to detect nerve agents. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY. HOBOKEN, NJ USA: WILEY, 2021, roč. 96, č. 6, s. 1758-1769. ISSN 0268-2575. doi:10.1002/jctb.6700.
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Základní údaje
Originální název The effect of different types of lactose monohydrate on the stability of acetylcholinesterase immobilized on carriers designed to detect nerve agents
Autoři ZEMAN, Jiří (203 Česká republika, domácí), Sylvie PAVLOKOVÁ (203 Česká republika, domácí), David VETCHÝ (203 Česká republika, garant, domácí) a Vladimír PITSCHMANN (203 Česká republika).
Vydání JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, HOBOKEN, NJ USA, WILEY, 2021, 0268-2575.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 30104 Pharmacology and pharmacy
Stát vydavatele Spojené státy
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 3.709
Kód RIV RIV/00216224:14160/21:00120053
Organizační jednotka Farmaceutická fakulta
Doi http://dx.doi.org/10.1002/jctb.6700
UT WoS 000625465600001
Klíčová slova anglicky acetylcholinesterase; detection tube; Ellman' s reaction; lactose; soman; stability
Štítky rivok, ÚFT
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: JUDr. Sabina Krejčiříková, učo 383857. Změněno: 14. 4. 2022 15:26.
Anotace
BACKGROUND Maintaining the stability of an immobilized enzyme during storage is generally very problematic. However, various substances have been reported to have a positive effect on enzyme stability. These include reducing disaccharide lactose, which is used in carriers in the form of pellets with immobilized cholinesterase. These carriers serve as a filling for detection tubes (biosensor), which are used for detecting nerve agents, wherein the detection itself is based on a colorimetric Ellman's reaction. To date, however, no study has been performed on the effect of different types of lactose monohydrate on the properties of such carriers. RESULTS The effect of five different types of lactose monohydrate on carrier properties and enzyme stability was examined. The lactose powders used differed in the particle size distribution, shape, compactability and flowability. The carriers were prepared in triplicate and subjected to a 24-month stability test under three storage conditions, with the results being statistically analyzed by analysis of variance, principal component analysis and t-test. Samples containing lactose with spherical particles were more resistant to degradation by heat and moisture than samples containing crystalline particles; in addition they had higher hardness and better sensitivity. All samples were also tested with soman after 24 months of storage and were found to retain very good sensitivity. CONCLUSION Samples containing spherical types of lactose proved to be more stable in some critical parameters compared to the samples with lactose in the form of single crystals, which are currently used in commercially available detection tubes. (c) 2021 Society of Chemical Industry (SCI).
Návaznosti
VI20192022172, projekt VaVNázev: Detekční trubičky druhé generace pro kontinuální a opakované monitorování nervově paralytických a jiných toxických látek
Investor: Ministerstvo vnitra ČR, Detekční trubičky druhé generace pro kontinuální a opakované monitorování nervově paralytických a jiných toxických látek
VytisknoutZobrazeno: 2. 2. 2023 15:01