J 2021

Eudiplozoon nipponicum (Monogenea, Diplozoidae) and its adaptation to haematophagy as revealed by transcriptome and secretome profiling

VOREL, Jiří, Krystyna CWIKLINSKI, Pavel ROUDNICKÝ, Jana ILGOVÁ, Lucie JEDLIČKOVÁ et. al.

Basic information

Original name

Eudiplozoon nipponicum (Monogenea, Diplozoidae) and its adaptation to haematophagy as revealed by transcriptome and secretome profiling

Authors

VOREL, Jiří (203 Czech Republic, guarantor, belonging to the institution), Krystyna CWIKLINSKI (826 United Kingdom of Great Britain and Northern Ireland), Pavel ROUDNICKÝ (203 Czech Republic, belonging to the institution), Jana ILGOVÁ (703 Slovakia, belonging to the institution), Lucie JEDLIČKOVÁ, John P. DALTON (826 United Kingdom of Great Britain and Northern Ireland), Libor MIKEŠ (203 Czech Republic), Milan GELNAR (203 Czech Republic, belonging to the institution) and Martin KAŠNÝ (203 Czech Republic, belonging to the institution)

Edition

BMC Genomics, London, BioMed Central, 2021, 1471-2164

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10603 Genetics and heredity

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 4.547

RIV identification code

RIV/00216224:14310/21:00118968

Organization unit

Faculty of Science

DOI

UT WoS

000641437500001

Keywords in English

Eudiplozoon nipponicum; Monogenea; NGS; Transcriptome; Assembly; Annotation; Secretome; Massspectrometry

Tags

Tags

International impact, Reviewed
Změněno: 10/10/2024 14:42, Ing. Martina Blahová

Abstract

V originále

Background: Ectoparasites from the family Diplozoidae (Platyhelminthes, Monogenea) belong to obligate haematophagous helminths of cyprinid fish. Current knowledge of these worms is for the most part limited to their morphological, phylogenetic, and population features. Information concerning the biochemical and molecular nature of physiological processes involved in host–parasite interaction, such as evasion of the immune system and its regulation, digestion of macromolecules, suppression of blood coagulation and inflammation, and effect on host tissue and physiology, is lacking. In this study, we report for the first time a comprehensive transcriptomic/secretome description of expressed genes and proteins secreted by the adult stage of Eudiplozoon nipponicum (Goto, 1891) Khotenovsky, 1985, an obligate sanguivorous monogenean which parasitises the gills of the common carp (Cyprinus carpio). Results: RNA-seq raw reads (324,941 Roche 454 and 149,697,864 Illumina) were generated, de novo assembled, and filtered into 37,062 protein-coding transcripts. For 19,644 (53.0%) of them, we determined their sequential homologues. In silico functional analysis of E. nipponicum RNA-seq data revealed numerous transcripts, pathways, and GO terms responsible for immunomodulation (inhibitors of proteolytic enzymes, CD59-like proteins, fatty acid binding proteins), feeding (proteolytic enzymes cathepsins B, D, L1, and L3), and development (fructose 1,6-bisphosphatase, ferritin, and annexin). LC-MS/MS spectrometry analysis identified 721 proteins secreted by E. nipponicum with predominantly immunomodulatory and anti-inflammatory functions (peptidyl-prolyl cis-trans isomerase, homolog to SmKK7, tetraspanin) and ability to digest host macromolecules (cathepsins B, D, L1). Conclusions: In this study, we integrated two high-throughput sequencing techniques, mass spectrometry analysis, and comprehensive bioinformatics approach in order to arrive at the first comprehensive description of monogenean transcriptome and secretome. Exploration of E. nipponicum transcriptome-related nucleotide sequences and translated and secreted proteins offer a better understanding of molecular biology and biochemistry of these, often neglected, organisms. It enabled us to report the essential physiological pathways and protein molecules involved in their interactions with the fish hosts.

Links

GAP506/12/1258, research and development project
Name: Interakce hostitel-parazit u krevsajících diplozoidních monogeneí: Výzkum vysoce specializovaných adaptací k parazitismu
Investor: Czech Science Foundation
GBP505/12/G112, research and development project
Name: ECIP - Evropské centrum ichtyoparazitologie
Investor: Czech Science Foundation
LM2018127, research and development project
Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)
Investor: Ministry of Education, Youth and Sports of the CR
LM2018131, research and development project
Name: Česká národní infrastruktura pro biologická data (Acronym: ELIXIR-CZ)
Investor: Ministry of Education, Youth and Sports of the CR, Czech National Infrastructure for Biological Data
LM2018140, research and development project
Name: e-Infrastruktura CZ (Acronym: e-INFRA CZ)
Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/A/0816/2017, interní kód MU
Name: Výzkum ekologicko-evolučních vztahů bezobratlých živočichů (Acronym: EKOLINKS)
Investor: Masaryk University, Category A
MUNI/A/0918/2018, interní kód MU
Name: Studium evolučních a ekologických vztahů a procesů u akvatických bezobratlých živočichů a v hostitelsko-parazitických systémech (Acronym: EvolEcolParaHydro)
Investor: Masaryk University, Category A
MUNI/A/1325/2015, interní kód MU
Name: Analýzy diverzity biologických systémů různých úrovní a na různých škálách prostředí (Acronym: BIDA5)
Investor: Masaryk University, Category A
MUNI/A/1362/2016, interní kód MU
Name: Biologická diverzita – analýzy biologických systémů různých úrovní a na různých škálách prostředí
Investor: Masaryk University, Category A
90127, large research infrastructures
Name: CIISB II