FIŠAROVÁ, Lenka, Tibor BOTKA, Xin DU, Ivana MAŠLAŇOVÁ, Pavol BÁRDY, Roman PANTŮČEK, Martin BENEŠÍK, Pavel ROUDNICKÝ, Volker WINSTEL, Jesper LARSEN, Ralf ROSENSTEIN, Andreas PESCHEL and Jiří DOŠKAŘ. Staphylococcus epidermidis Phages Transduce Antimicrobial Resistance Plasmids and Mobilize Chromosomal Islands. mSphere. Washington, DC, USA: American Society for Microbiology, 2021, vol. 6, No 3, p. 1-19. ISSN 2379-5042. doi:10.1128/mSphere.00223-21.
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Basic information
Original name Staphylococcus epidermidis Phages Transduce Antimicrobial Resistance Plasmids and Mobilize Chromosomal Islands
Authors FIŠAROVÁ, Lenka (203 Czech Republic, belonging to the institution), Tibor BOTKA (203 Czech Republic, belonging to the institution), Xin DU (156 China), Ivana MAŠLAŇOVÁ (203 Czech Republic, belonging to the institution), Pavol BÁRDY (703 Slovakia, belonging to the institution), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Martin BENEŠÍK (203 Czech Republic, belonging to the institution), Pavel ROUDNICKÝ (203 Czech Republic, belonging to the institution), Volker WINSTEL (276 Germany), Jesper LARSEN (208 Denmark), Ralf ROSENSTEIN (276 Germany), Andreas PESCHEL (276 Germany) and Jiří DOŠKAŘ (203 Czech Republic, guarantor, belonging to the institution).
Edition mSphere, Washington, DC, USA, American Society for Microbiology, 2021, 2379-5042.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10608 Biochemistry and molecular biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 5.029
RIV identification code RIV/00216224:14310/21:00118971
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1128/mSphere.00223-21
UT WoS 000663083400008
Keywords in English bacteriophages; Staphylococcus epidermidis; antibiotic resistance; horizontal gene transfer; pathogenicity islands; transduction
Tags CF CRYO, CF GEN, CF PROT, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 28/4/2022 09:38.
Abstract
Staphylococcus epidermidis is a leading opportunistic pathogen causing nosocomial infections that is notable for its ability to form a biofilm and for its high rates of antibiotic resistance. It serves as a reservoir of multiple antimicrobial resistance genes that spread among the staphylococcal population by horizontal gene transfer such as transduction. While phage-mediated transduction is well studied in Staphylococcus aureus, S. epidermidis transducing phages have not been described in detail yet. Here, we report the characteristics of four phages, 27, 48, 456, and 459, previously used for S. epidermidis phage typing, and the newly isolated phage E72, from a clinical S. epidermidis strain. The phages, classified in the family Siphoviridae and genus Phietavirus, exhibited an S. epidermidis-specific host range, and together they infected 49% of the 35 strains tested. A whole-genome comparison revealed evolutionary relatedness to transducing S. aureus phietaviruses. In accordance with this, all the tested phages were capable of transduction with high frequencies up to 10−4 among S. epidermidis strains from different clonal complexes. Plasmids with sizes from 4 to 19 kb encoding resistance to streptomycin, tetracycline, and chloramphenicol were transferred. We provide here the first evidence of a phage-inducible chromosomal island transfer in S. epidermidis. Similarly to S. aureus pathogenicity islands, the transfer was accompanied by phage capsid remodeling; however, the interfering protein encoded by the island was distinct. Our findings underline the role of S. epidermidis temperate phages in the evolution of S. epidermidis strains by horizontal gene transfer, which can also be utilized for S. epidermidis genetic studies.
Links
GA18-13064S, research and development projectName: Analýza interakcí mezi polyvalentním terapeutickým fágem druhu Twort-like a jeho hostitelem Staphylococcus aureus
Investor: Czech Science Foundation
LM2018127, research and development projectName: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)
Investor: Ministry of Education, Youth and Sports of the CR
LM2018132, research and development projectName: Národní centrum lékařské genomiky (Acronym: NCLG)
Investor: Ministry of Education, Youth and Sports of the CR, National Center for Medical Genomics
LQ1601, research and development projectName: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/A/1522/2020, interní kód MUName: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 9 (Acronym: MBG9)
Investor: Masaryk University
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