TNFR2 expression is a hallmark of human memory B cells with
suppressive function

J 2021

TNFR2 expression is a hallmark of human memory B cells with suppressive function

TICHA, Olga, Peter SLANINA, Lukas MOOS, Julie ŠTÍCHOVÁ, Marcela VLKOVÁ et. al.

Basic information

Original name

TNFR2 expression is a hallmark of human memory B cells with suppressive function

Authors

TICHA, Olga (203 Czech Republic), Peter SLANINA (703 Slovakia, belonging to the institution), Lukas MOOS, Julie ŠTÍCHOVÁ (203 Czech Republic, belonging to the institution), Marcela VLKOVÁ (203 Czech Republic, belonging to the institution) and Isabelle BEKEREDJIAN-DING (guarantor)

Edition

EUROPEAN JOURNAL OF IMMUNOLOGY, HOBOKEN, WILEY, 2021, 0014-2980

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30102 Immunology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.688

RIV identification code

RIV/00216224:14110/21:00121608

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1002/eji.202048988

UT WoS

000626550800001

Keywords in English

B cells; Breg; IL‐ 10; TLR9; TNFR2

Abstract

V originále

Tumor Necrosis Factor Receptor 2 (TNFR2) expression is increasingly being linked to tolerogenic immune reactions and cells with suppressor function including a subset of T-regulatory cells. B-regulatory cells play an important role in control of T-cell responses and inflammation. Recently, we described TNFR2 as a marker for IL-10-producing B cells, a hallmark of this cell subset. Here, we demonstrate that proliferation of T cells is reduced in the presence of TNFR2 positive human memory B cells generated with TLR9 ligand, while TNFR2- and TNFR2+CD27- B cells display costimulatory activity. Our data further reveal that IL-10 secretion is characteristic of IgM+ naive and memory B cells but suppressive activity is not restricted to IL-10: (i) the inhibitory effect of TNFR2+ switched memory B cells was comparable to that exerted by TNFR2+ IgM+ memory B cells although IL-10 secretion levels in the cocultures were lower; (ii) supernatants from TNFR2+ memory B cells failed to suppress T-cell proliferation. Based on our findings, we propose that formation of Breg is a specific characteristic of human memory B cells undergoing terminal differentiation. Our data further corroborate that TNFR2 represents a viable marker for identification of memory B cells with regulatory function.

Citovat

TICHA, Olga, Peter SLANINA, Lukas MOOS, Julie ŠTÍCHOVÁ, Marcela VLKOVÁ and Isabelle BEKEREDJIAN-DING. TNFR2 expression is a hallmark of human memory B cells with suppressive function. EUROPEAN JOURNAL OF IMMUNOLOGY. HOBOKEN: WILEY, 2021, vol. 51, No 5, p. 1195-1205. ISSN 0014-2980. Available from: https://dx.doi.org/10.1002/eji.202048988.

@article{1769698,
   author = {Ticha, Olga and Slanina, Peter and Moos, Lukas and Štíchová, Julie and Vlková, Marcela and BekeredjianandDing, Isabelle},
   article_location = {HOBOKEN},
   article_number = {5},
   doi = {http://dx.doi.org/10.1002/eji.202048988},
   keywords = {B cells; Breg; IL‐ 10; TLR9; TNFR2},
   language = {eng},
   issn = {0014-2980},
   journal = {EUROPEAN JOURNAL OF IMMUNOLOGY},
   title = {TNFR2 expression is a hallmark of human memory B cells with suppressive function},
   url = {https://onlinelibrary.wiley.com/journal/15214141},
   volume = {51},
   year = {2021}
}

TY  - JOUR
ID  - 1769698
AU  - Ticha, Olga - Slanina, Peter - Moos, Lukas - Štíchová, Julie - Vlková, Marcela - Bekeredjian-Ding, Isabelle
PY  - 2021
TI  - TNFR2 expression is a hallmark of human memory B cells with suppressive function
JF  - EUROPEAN JOURNAL OF IMMUNOLOGY
VL  - 51
IS  - 5
SP  - 1195-1205
EP  - 1195-1205
PB  - WILEY
SN  - 00142980
KW  - B cells
KW  - Breg
KW  - IL‐ 10
KW  - TLR9
KW  - TNFR2
UR  - https://onlinelibrary.wiley.com/journal/15214141
N2  - Tumor Necrosis Factor Receptor 2 (TNFR2) expression is increasingly being linked to tolerogenic immune reactions and cells with suppressor function including a subset of T-regulatory cells. B-regulatory cells play an important role in control of T-cell responses and inflammation. Recently, we described TNFR2 as a marker for IL-10-producing B cells, a hallmark of this cell subset. Here, we demonstrate that proliferation of T cells is reduced in the presence of TNFR2 positive human memory B cells generated with TLR9 ligand, while TNFR2- and TNFR2+CD27- B cells display costimulatory activity. Our data further reveal that IL-10 secretion is characteristic of IgM+ naive and memory B cells but suppressive activity is not restricted to IL-10: (i) the inhibitory effect of TNFR2+ switched memory B cells was comparable to that exerted by TNFR2+ IgM+ memory B cells although IL-10 secretion levels in the cocultures were lower; (ii) supernatants from TNFR2+ memory B cells failed to suppress T-cell proliferation. Based on our findings, we propose that formation of Breg is a specific characteristic of human memory B cells undergoing terminal differentiation. Our data further corroborate that TNFR2 represents a viable marker for identification of memory B cells with regulatory function.
ER  -

TICHA, Olga, Peter SLANINA, Lukas MOOS, Julie ŠTÍCHOVÁ, Marcela VLKOVÁ and Isabelle BEKEREDJIAN-DING. TNFR2 expression is a hallmark of human memory B cells with suppressive function. \textit{EUROPEAN JOURNAL OF IMMUNOLOGY}. HOBOKEN: WILEY, 2021, vol.~51, No~5, p.~1195-1205. ISSN~0014-2980. Available from: https://dx.doi.org/10.1002/eji.202048988.

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