CAR T-Cell Production Using Nonviral Approaches

J 2021

CAR T-Cell Production Using Nonviral Approaches

LUKJANOV, Viktor, Irena KOUTNÁ and Pavel ŠIMARA

Basic information

Original name

CAR T-Cell Production Using Nonviral Approaches

Name in Czech

Výroba CAR T-lymfocytů pomocí nevirových metod

Authors

LUKJANOV, Viktor (203 Czech Republic, belonging to the institution), Irena KOUTNÁ (203 Czech Republic, belonging to the institution) and Pavel ŠIMARA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Immunology Research, London, Hindawi, 2021, 2314-8861

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30102 Immunology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Hindawi

Impact factor

Impact factor: 4.493

RIV identification code

RIV/00216224:14110/21:00120099

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1155/2021/6644685

UT WoS

000637765000002

Keywords (in Czech)

CAR T-lymfocyty; PiggyBac

Keywords in English

CAR T-cells; PiggyBac

Abstract

V originále

Chimeric antigen receptor T-cells (CAR T-cells) represent a novel and promising approach in cancer immunotherapy. According to the World Health Organization (WHO), the number of oncological patients is steadily growing in developed countries despite immense progress in oncological treatments, and the prognosis of individual patients is still relatively poor. Exceptional results have been recorded for CAR T-cell therapy in patients suffering from B-cell malignancies. This success opens up the possibility of using the same approach for other types of cancers. To date, the most common method for CAR T-cell generation is the use of viral vectors. However, dealing with virus-derived vectors brings possible obstacles in the CAR T-cell manufacturing process owing to strict regulations and high cost demands. Alternative approaches may facilitate further development and the transfer of the method to clinical practice. The most promising substitutes for virus-derived vectors are transposon-derived vectors, most commonly sleeping beauty, which offer great coding capability and a safe integration profile while maintaining a relatively low production cost. This review is aimed at summarizing the state of the art of nonviral approaches in CAR T-cell generation, with a unique perspective on the conditions in clinical applications and current Good Manufacturing Practice. If CAR T-cell therapy is to be routinely used in medical practice, the manufacturing cost and complexity need to be as low as possible, and transposon-based vectors seem to meet these criteria better than viral-based vectors.

Links

NV19-08-00147, research and development project

Name: Pre-klinická validace cGMP produkce CAR T-lymfocytů pro léčbu solidních tumorů

Investor: Ministry of Health of the CR

Citovat

LUKJANOV, Viktor, Irena KOUTNÁ and Pavel ŠIMARA. CAR T-Cell Production Using Nonviral Approaches. Journal of Immunology Research. London: Hindawi, 2021, vol. 2021, Mar 27, p. 1-9. ISSN 2314-8861. Available from: https://dx.doi.org/10.1155/2021/6644685.

@article{1769838,
   author = {Lukjanov, Viktor and Koutná, Irena and Šimara, Pavel},
   article_location = {London},
   article_number = {Mar 27},
   doi = {http://dx.doi.org/10.1155/2021/6644685},
   keywords = {CAR T-cells; PiggyBac},
   language = {eng},
   issn = {2314-8861},
   journal = {Journal of Immunology Research},
   title = {CAR T-Cell Production Using Nonviral Approaches},
   url = {https://www.hindawi.com/journals/jir/2021/6644685/},
   volume = {2021},
   year = {2021}
}

TY  - JOUR
ID  - 1769838
AU  - Lukjanov, Viktor - Koutná, Irena - Šimara, Pavel
PY  - 2021
TI  - CAR T-Cell Production Using Nonviral Approaches
JF  - Journal of Immunology Research
VL  - 2021
IS  - Mar 27
SP  - 1-9
EP  - 1-9
PB  - Hindawi
SN  - 23148861
KW  - CAR T-cells
KW  - PiggyBac
UR  - https://www.hindawi.com/journals/jir/2021/6644685/
N2  - Chimeric antigen receptor T-cells (CAR T-cells) represent a novel and promising approach in cancer immunotherapy. According to the World Health Organization (WHO), the number of oncological patients is steadily growing in developed countries despite immense progress in oncological treatments, and the prognosis of individual patients is still relatively poor. Exceptional results have been recorded for CAR T-cell therapy in patients suffering from B-cell malignancies. This success opens up the possibility of using the same approach for other types of cancers. To date, the most common method for CAR T-cell generation is the use of viral vectors. However, dealing with virus-derived vectors brings possible obstacles in the CAR T-cell manufacturing process owing to strict regulations and high cost demands. Alternative approaches may facilitate further development and the transfer of the method to clinical practice. The most promising substitutes for virus-derived vectors are transposon-derived vectors, most commonly sleeping beauty, which offer great coding capability and a safe integration profile while maintaining a relatively low production cost. This review is aimed at summarizing the state of the art of nonviral approaches in CAR T-cell generation, with a unique perspective on the conditions in clinical applications and current Good Manufacturing Practice. If CAR T-cell therapy is to be routinely used in medical practice, the manufacturing cost and complexity need to be as low as possible, and transposon-based vectors seem to meet these criteria better than viral-based vectors.
ER  -

LUKJANOV, Viktor, Irena KOUTNÁ and Pavel ŠIMARA. CAR T-Cell Production Using Nonviral Approaches. \textit{Journal of Immunology Research}. London: Hindawi, 2021, vol.~2021, Mar 27, p.~1-9. ISSN~2314-8861. Available from: https://dx.doi.org/10.1155/2021/6644685.

Detailed Information on Publication Record