The biological activity of the organic UV filter ethylhexyl
methoxycinnamate in rainbow trout (Oncorhynchus mykiss)

J 2021

The biological activity of the organic UV filter ethylhexyl methoxycinnamate in rainbow trout (Oncorhynchus mykiss)

CAHOVA, Jana, Jana BLAHOVA, Petr MARSALEK, Veronika DOUBKOVA, Aleš FRANC et. al.

Basic information

Original name

The biological activity of the organic UV filter ethylhexyl methoxycinnamate in rainbow trout (Oncorhynchus mykiss)

Authors

CAHOVA, Jana (203 Czech Republic), Jana BLAHOVA (203 Czech Republic, guarantor), Petr MARSALEK (203 Czech Republic), Veronika DOUBKOVA (203 Czech Republic), Aleš FRANC (203 Czech Republic, belonging to the institution), Michaela GARAJOVÁ (703 Slovakia, belonging to the institution), Frantisek TICHY (203 Czech Republic), Jan MARES (203 Czech Republic) and Zdenka SVOBODOVA (203 Czech Republic)

Edition

Science of the Total Environment, Amsterdam, Elsevier Science, 2021, 0048-9697

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30108 Toxicology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 10.753

RIV identification code

RIV/00216224:14160/21:00121636

Organization unit

Faculty of Pharmacy

DOI

http://dx.doi.org/10.1016/j.scitotenv.2021.145570

UT WoS

000641160800019

Keywords in English

EHMC; Fish; Aquatic ecosystem; Oxidative stress; Haematological and biochemical indices; Histopathology

Abstract

V originále

UV filters are able to enter the aquatic environment and negatively affect non-target organisms. The aim of this study was to evaluate subchronic exposure to ethylhexyl methoxycinnamate (EHMC) in rainbow trout. The tested EHMC concentrations of 6.9 (low), 96.0 (medium) and 395.6 mu g/kg (high) were used. The lowest concentration was based on environmentally relevant concentrations. The higher concentrations were chosen as a multiple of the lowest one to determine the dose-response relationship. EHMC was incorporated into feed pellets. The experiment was conducted for six weeks in a semi-static system. Haematological, biochemical and oxidative stress indices were determined at the end of the experiment and supplemented by histological examination. Significant changes were proven at medium and high concentrations of EHMC. Specifically, increases of glucose, lactate and decrease of albumin and total protein in plasma indicate a stress ethology. Moreover, a decrease of plasma cholesterol, triacylglycerols and ammonia were observed even in the experimental group exposed to the lowest concentration of EHMC, perceived as an environmentally relevant concentration. The ferric reducing ability of plasma was decreased in all tested concentrations. Exposure to the highest concentration of EHMC resulted in a decrease in leukocyte counts. Increased activity of glutathione peroxidase in liver was recorded for the medium and the highest concentration of EHMC. The level of the thiobarbituric acid reactive substances in kidney was elevated for the highest concentration. Decrease of the activity of glutathione-S-transferase in gills for medium concentration of EHMC was registered. Histopathological examination revealed massive destruction of hepatic parenchyma at the highest concentration of EHMC. All these results support the finding of a stress load on the fish organism. In summary, although subchronic exposure to EHMC had no effect on behaviour, mortality or feed intake, this exposure resulted in the alteration of saccharide, lipid and protein metabolism and weakened antioxidant capacity. (c) 2021 Elsevier B.V. All rights reserved.

Citovat

CAHOVA, Jana, Jana BLAHOVA, Petr MARSALEK, Veronika DOUBKOVA, Aleš FRANC, Michaela GARAJOVÁ, Frantisek TICHY, Jan MARES and Zdenka SVOBODOVA. The biological activity of the organic UV filter ethylhexyl methoxycinnamate in rainbow trout (Oncorhynchus mykiss). Science of the Total Environment. Amsterdam: Elsevier Science, 2021, vol. 774, No 145570, p. 1-8. ISSN 0048-9697. Available from: https://dx.doi.org/10.1016/j.scitotenv.2021.145570.

@article{1770937,
   author = {Cahova, Jana and Blahova, Jana and Marsalek, Petr and Doubkova, Veronika and Franc, Aleš and Garajová, Michaela and Tichy, Frantisek and Mares, Jan and Svobodova, Zdenka},
   article_location = {Amsterdam},
   article_number = {145570},
   doi = {http://dx.doi.org/10.1016/j.scitotenv.2021.145570},
   keywords = {EHMC; Fish; Aquatic ecosystem; Oxidative stress; Haematological and biochemical indices; Histopathology},
   language = {eng},
   issn = {0048-9697},
   journal = {Science of the Total Environment},
   title = {The biological activity of the organic UV filter ethylhexyl methoxycinnamate in rainbow trout (Oncorhynchus mykiss)},
   url = {https://www.sciencedirect.com/science/article/pii/S0048969721006380?via%3Dihub},
   volume = {774},
   year = {2021}
}

TY  - JOUR
ID  - 1770937
AU  - Cahova, Jana - Blahova, Jana - Marsalek, Petr - Doubkova, Veronika - Franc, Aleš - Garajová, Michaela - Tichy, Frantisek - Mares, Jan - Svobodova, Zdenka
PY  - 2021
TI  - The biological activity of the organic UV filter ethylhexyl methoxycinnamate in rainbow trout (Oncorhynchus mykiss)
JF  - Science of the Total Environment
VL  - 774
IS  - 145570
SP  - 1-8
EP  - 1-8
PB  - Elsevier Science
SN  - 00489697
KW  - EHMC
KW  - Fish
KW  - Aquatic ecosystem
KW  - Oxidative stress
KW  - Haematological and biochemical indices
KW  - Histopathology
UR  - https://www.sciencedirect.com/science/article/pii/S0048969721006380?via%3Dihub
N2  - UV filters are able to enter the aquatic environment and negatively affect non-target organisms. The aim of this study was to evaluate subchronic exposure to ethylhexyl methoxycinnamate (EHMC) in rainbow trout. The tested EHMC concentrations of 6.9 (low), 96.0 (medium) and 395.6 mu g/kg (high) were used. The lowest concentration was based on environmentally relevant concentrations. The higher concentrations were chosen as a multiple of the lowest one to determine the dose-response relationship. EHMC was incorporated into feed pellets. The experiment was conducted for six weeks in a semi-static system. Haematological, biochemical and oxidative stress indices were determined at the end of the experiment and supplemented by histological examination. Significant changes were proven at medium and high concentrations of EHMC. Specifically, increases of glucose, lactate and decrease of albumin and total protein in plasma indicate a stress ethology. Moreover, a decrease of plasma cholesterol, triacylglycerols and ammonia were observed even in the experimental group exposed to the lowest concentration of EHMC, perceived as an environmentally relevant concentration. The ferric reducing ability of plasma was decreased in all tested concentrations. Exposure to the highest concentration of EHMC resulted in a decrease in leukocyte counts. Increased activity of glutathione peroxidase in liver was recorded for the medium and the highest concentration of EHMC. The level of the thiobarbituric acid reactive substances in kidney was elevated for the highest concentration. Decrease of the activity of glutathione-S-transferase in gills for medium concentration of EHMC was registered. Histopathological examination revealed massive destruction of hepatic parenchyma at the highest concentration of EHMC. All these results support the finding of a stress load on the fish organism. In summary, although subchronic exposure to EHMC had no effect on behaviour, mortality or feed intake, this exposure resulted in the alteration of saccharide, lipid and protein metabolism and weakened antioxidant capacity. (c) 2021 Elsevier B.V. All rights reserved.
ER  -

CAHOVA, Jana, Jana BLAHOVA, Petr MARSALEK, Veronika DOUBKOVA, Aleš FRANC, Michaela GARAJOVÁ, Frantisek TICHY, Jan MARES and Zdenka SVOBODOVA. The biological activity of the organic UV filter ethylhexyl methoxycinnamate in rainbow trout (Oncorhynchus mykiss). \textit{Science of the Total Environment}. Amsterdam: Elsevier Science, 2021, vol.~774, No~145570, p.~1-8. ISSN~0048-9697. Available from: https://dx.doi.org/10.1016/j.scitotenv.2021.145570.

Detailed Information on Publication Record